Staff View: Inhibitory and anti-adherent effects of piper betle l. Leaf extract against acanthamoeba triangularis in co-infection with staphylococcus aureus and pseudomonas aeruginosa: A sustainable one-health approach

Inhibitory and anti-adherent effects of piper betle l. Leaf extract against acanthamoeba triangularis in co-infection with staphylococcus aureus and pseudomonas aeruginosa: A sustainable one-health approach

Background and Aim: Keratitis is a serious ocular infection often caused by pathogenic microorganisms such as Acanthamoeba spp. Among other harmful microbes, Acanthamoeba keratitis presents a particular challenge due to its resistance to conventional antimicrobial agents. Piper betle Linn., commonly...

Full description

Saved in:

Bibliographic Details
Main Authors:	Pattamaporn Kwankaew, Suthinee Sangkanu, Watcharapong Mitsuwan, Rachasak Boonhok, Udom Lao-On, Hazel L. Tabo, Tooba Mahboob, Maria de Lourdes Pereira, Jitbanjong Tangpong, Shanmuga S. Sundar, Christophe Wiart, Veeranoot Nissapatorn
Format:	Article
Language:	English English
Published:	Veterinary World 2024
Subjects:	QL360-599.82 Invertebrates QR75-99.5 Bacteria
Online Access:	https://eprints.ums.edu.my/id/eprint/42003/1/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/42003/2/FULL%20TEXT.pdf https://eprints.ums.edu.my/id/eprint/42003/ https://doi.org/10.14202/vetworld.2024.848-862
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my.ums.eprints.42003
record_format	eprints
spelling	my.ums.eprints.420032024-11-26T05:44:09Z https://eprints.ums.edu.my/id/eprint/42003/ Inhibitory and anti-adherent effects of piper betle l. Leaf extract against acanthamoeba triangularis in co-infection with staphylococcus aureus and pseudomonas aeruginosa: A sustainable one-health approach Pattamaporn Kwankaew Suthinee Sangkanu Watcharapong Mitsuwan Rachasak Boonhok Udom Lao-On Hazel L. Tabo Tooba Mahboob Maria de Lourdes Pereira Jitbanjong Tangpong Shanmuga S. Sundar Christophe Wiart Veeranoot Nissapatorn QL360-599.82 Invertebrates QR75-99.5 Bacteria Background and Aim: Keratitis is a serious ocular infection often caused by pathogenic microorganisms such as Acanthamoeba spp. Among other harmful microbes, Acanthamoeba keratitis presents a particular challenge due to its resistance to conventional antimicrobial agents. Piper betle Linn., commonly known as betel leaf, has been traditionally used for its medicinal properties. This study aimed to assess the potential of the leaf ethanol extract of P. betle Linn. in the treatment of Acanthamoeba triangularis in monoculture and co-culture with two prevalent pathogenic bacteria, Staphylococcus aureus and Pseudomonas aeruginosa, associated with keratitis. Materials and Methods: Minimum inhibitory concentrations (MICs) of A. triangularis, S. aureus, and P. aeruginosa extracts in monoculture and coinfected conditions were examined. In addition, this study explored the potential of the extract in preventing Acanthamoeba adherence in both monoculture and co-culture environments. Scanning electron microscopy (SEM) analysis confirmed the impact of the extract on Acanthamoeba cell membranes, including acanthopodia. Furthermore, a timekill kinetic assay was used to validate the amoebicidal activity of the extract against A. triangularis and the tested bacteria. Results: MICs for trophozoites, cysts, P. aeruginosa, and S. aureus in the monoculture were 0.25, 0.25, 0.51, and 0.128mg/mL, respectively, whereas the MICs for Acanthamoeba coinfected with bacteria were higher than those in the monoculture. This extract inhibited the growth of A. triangularis trophozoites and cysts for up to 72 h. Moreover, P. betle extract effectively prevented the adherence of Acanthamoeba to contact lenses under monoculture conditions. SEM analysis confirmed that P. betle extract affects the cell membrane of Acanthamoeba, including Acanthopodia. In addition, the time-kill kinetic assay confirmed that the extract contained amoebicidal activity against A. triangularis, including the tested bacteria. Notably, S. aureus was more susceptible than A. triangularis and P. aeruginosa to P. betle extract treatment. Unexpectedly, our study revealed that S. aureus negatively affected A. triangularis in the co-culture after 3 days of incubation, whereas P. aeruginosa facilitated the growth of A. triangularis in the presence of the extract. Conclusion: This study provides compelling evidence of the anti-adhesive and anti-Acanthamoeba properties of P. betle leaf extract against A. triangularis under monoculture and co-culture conditions. The observed impact on Acanthamoeba cell membranes, coupled with the time-kill kinetic assay results, underscores the potential of P. betle leaf extract as a promising agent for combating Acanthamoeba-related infections in humans and animals. Veterinary World 2024 Article NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/42003/1/ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/42003/2/FULL%20TEXT.pdf Pattamaporn Kwankaew and Suthinee Sangkanu and Watcharapong Mitsuwan and Rachasak Boonhok and Udom Lao-On and Hazel L. Tabo and Tooba Mahboob and Maria de Lourdes Pereira and Jitbanjong Tangpong and Shanmuga S. Sundar and Christophe Wiart and Veeranoot Nissapatorn (2024) Inhibitory and anti-adherent effects of piper betle l. Leaf extract against acanthamoeba triangularis in co-infection with staphylococcus aureus and pseudomonas aeruginosa: A sustainable one-health approach. Veterinary World, 17. pp. 1-15. ISSN 2231-0916 https://doi.org/10.14202/vetworld.2024.848-862
institution	Universiti Malaysia Sabah
building	UMS Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaysia Sabah
content_source	UMS Institutional Repository
url_provider	http://eprints.ums.edu.my/
language	English English
topic	QL360-599.82 Invertebrates QR75-99.5 Bacteria
spellingShingle	QL360-599.82 Invertebrates QR75-99.5 Bacteria Pattamaporn Kwankaew Suthinee Sangkanu Watcharapong Mitsuwan Rachasak Boonhok Udom Lao-On Hazel L. Tabo Tooba Mahboob Maria de Lourdes Pereira Jitbanjong Tangpong Shanmuga S. Sundar Christophe Wiart Veeranoot Nissapatorn Inhibitory and anti-adherent effects of piper betle l. Leaf extract against acanthamoeba triangularis in co-infection with staphylococcus aureus and pseudomonas aeruginosa: A sustainable one-health approach
description	Background and Aim: Keratitis is a serious ocular infection often caused by pathogenic microorganisms such as Acanthamoeba spp. Among other harmful microbes, Acanthamoeba keratitis presents a particular challenge due to its resistance to conventional antimicrobial agents. Piper betle Linn., commonly known as betel leaf, has been traditionally used for its medicinal properties. This study aimed to assess the potential of the leaf ethanol extract of P. betle Linn. in the treatment of Acanthamoeba triangularis in monoculture and co-culture with two prevalent pathogenic bacteria, Staphylococcus aureus and Pseudomonas aeruginosa, associated with keratitis. Materials and Methods: Minimum inhibitory concentrations (MICs) of A. triangularis, S. aureus, and P. aeruginosa extracts in monoculture and coinfected conditions were examined. In addition, this study explored the potential of the extract in preventing Acanthamoeba adherence in both monoculture and co-culture environments. Scanning electron microscopy (SEM) analysis confirmed the impact of the extract on Acanthamoeba cell membranes, including acanthopodia. Furthermore, a timekill kinetic assay was used to validate the amoebicidal activity of the extract against A. triangularis and the tested bacteria. Results: MICs for trophozoites, cysts, P. aeruginosa, and S. aureus in the monoculture were 0.25, 0.25, 0.51, and 0.128mg/mL, respectively, whereas the MICs for Acanthamoeba coinfected with bacteria were higher than those in the monoculture. This extract inhibited the growth of A. triangularis trophozoites and cysts for up to 72 h. Moreover, P. betle extract effectively prevented the adherence of Acanthamoeba to contact lenses under monoculture conditions. SEM analysis confirmed that P. betle extract affects the cell membrane of Acanthamoeba, including Acanthopodia. In addition, the time-kill kinetic assay confirmed that the extract contained amoebicidal activity against A. triangularis, including the tested bacteria. Notably, S. aureus was more susceptible than A. triangularis and P. aeruginosa to P. betle extract treatment. Unexpectedly, our study revealed that S. aureus negatively affected A. triangularis in the co-culture after 3 days of incubation, whereas P. aeruginosa facilitated the growth of A. triangularis in the presence of the extract. Conclusion: This study provides compelling evidence of the anti-adhesive and anti-Acanthamoeba properties of P. betle leaf extract against A. triangularis under monoculture and co-culture conditions. The observed impact on Acanthamoeba cell membranes, coupled with the time-kill kinetic assay results, underscores the potential of P. betle leaf extract as a promising agent for combating Acanthamoeba-related infections in humans and animals.
format	Article
author	Pattamaporn Kwankaew Suthinee Sangkanu Watcharapong Mitsuwan Rachasak Boonhok Udom Lao-On Hazel L. Tabo Tooba Mahboob Maria de Lourdes Pereira Jitbanjong Tangpong Shanmuga S. Sundar Christophe Wiart Veeranoot Nissapatorn
author_facet	Pattamaporn Kwankaew Suthinee Sangkanu Watcharapong Mitsuwan Rachasak Boonhok Udom Lao-On Hazel L. Tabo Tooba Mahboob Maria de Lourdes Pereira Jitbanjong Tangpong Shanmuga S. Sundar Christophe Wiart Veeranoot Nissapatorn
author_sort	Pattamaporn Kwankaew
title	Inhibitory and anti-adherent effects of piper betle l. Leaf extract against acanthamoeba triangularis in co-infection with staphylococcus aureus and pseudomonas aeruginosa: A sustainable one-health approach
title_short	Inhibitory and anti-adherent effects of piper betle l. Leaf extract against acanthamoeba triangularis in co-infection with staphylococcus aureus and pseudomonas aeruginosa: A sustainable one-health approach
title_full	Inhibitory and anti-adherent effects of piper betle l. Leaf extract against acanthamoeba triangularis in co-infection with staphylococcus aureus and pseudomonas aeruginosa: A sustainable one-health approach
title_fullStr	Inhibitory and anti-adherent effects of piper betle l. Leaf extract against acanthamoeba triangularis in co-infection with staphylococcus aureus and pseudomonas aeruginosa: A sustainable one-health approach
title_full_unstemmed	Inhibitory and anti-adherent effects of piper betle l. Leaf extract against acanthamoeba triangularis in co-infection with staphylococcus aureus and pseudomonas aeruginosa: A sustainable one-health approach
title_sort	inhibitory and anti-adherent effects of piper betle l. leaf extract against acanthamoeba triangularis in co-infection with staphylococcus aureus and pseudomonas aeruginosa: a sustainable one-health approach
publisher	Veterinary World
publishDate	2024
url	https://eprints.ums.edu.my/id/eprint/42003/1/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/42003/2/FULL%20TEXT.pdf https://eprints.ums.edu.my/id/eprint/42003/ https://doi.org/10.14202/vetworld.2024.848-862
_version_	1817843849082437632
score	13.239859

Inhibitory and anti-adherent effects of piper betle l. Leaf extract against acanthamoeba triangularis in co-infection with staphylococcus aureus and pseudomonas aeruginosa: A sustainable one-health approach

Similar Items