Cover Image

Antibacterial properties of bentonite based zeolite a ion exchanged with silver and copper against marine bacterial pathogens

Development of aquaculture industry has been increasing rapidly around the world. However, this development is facing a great challenge due to bacterial disease outbreaks, leading to high mortality rates of the aquaculture population. Attempts to overcome this problem has resulted in a new greater c...

Full description

Saved in:
Bibliographic Details
Main Author: Nur Ariffah Waly
Format: Thesis
Language:English
English
Published: 2020
Subjects:
RM300-666 Drugs and their actions
Online Access:https://eprints.ums.edu.my/id/eprint/41687/1/24%20PAGES.pdf
https://eprints.ums.edu.my/id/eprint/41687/2/FULLTEXT.pdf
https://eprints.ums.edu.my/id/eprint/41687/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.ums.eprints.41687
record_format eprints
spelling my.ums.eprints.416872024-11-26T06:04:14Z https://eprints.ums.edu.my/id/eprint/41687/ Antibacterial properties of bentonite based zeolite a ion exchanged with silver and copper against marine bacterial pathogens Nur Ariffah Waly RM300-666 Drugs and their actions Development of aquaculture industry has been increasing rapidly around the world. However, this development is facing a great challenge due to bacterial disease outbreaks, leading to high mortality rates of the aquaculture population. Attempts to overcome this problem has resulted in a new greater challenge of antimicrobial resistance of bacteria due to broad usage of antibiotics. Therefore, the development of inorganic antibacterial agent, through the synthesis of bentonite based Zeolite A, further integrated with Ag and Cu? via ion exchange was hence proposed as an alternative to combat the bacteria. The bentonite clay as Si-Al source for zeolite synthesis was thermally transformed into an active phase at 100 ·C for 24 hours in the presence of concentrated hydrochloric acid, followed with the preparation of zeolite A mixture. Mixture of Zeolite A was prepared by using 2.5NaO: Al5Os: 1.5Si0,: 166H0 molar ratio. Hydrothermal synthesis of Zeolite A was carried out by aging process for 30 minutes followed by crystallization for 8 hours at 100 C and it was found that the optimum silica to alumina ratio and molarity of NaOH for the synthesis was 1.50 and 2.50 M respectively. Subsequently, the Zeolite A product underwent ion-exchange process with Ag and Cu?'and the metal content of ion-exchanged zeolite was determined by ICP-OES. The results obtained shows that pH and concentration of metal ion solution played important parts in ion-exchange process. The highest Cu? content was found on Zeolite A that was treated with 1.50 M of copper solution at pH 6 which is equal to 93.630 ± 1.114 (mg/g). Meanwhile, the highest Ag+ content was found on Zeolite A that was introduced to 1.50 M of silver solution at pH 6 which is equal to 190.511 ± 1.989 (mg/g). Antibacterial activity of the Ag (Ag?) and Cu?' (CuZ) ionexchanged zeolite A samples against V. campbellii and V. parahaemolyticus were evaluated by determining the Minimum Inhibition Concentration (MICs) using twofold serial dilutions of ion loaded zeolites in Tryptic Soy broth (TSB) supplemented with 2% of NaCl. The concentrations for AgZ and CuZ ranged from 0.125 mg/ml - 2.00 mg/ml and 5.00 mg/ml - 25.00 mg/ml respectively. The resulting results obtained shows that, the highest metal ion content zeolite A gives the lowest MICs value. The highest silver ion content in zeolite A needed to inhibit the growth of V. campbe/lii and V. parahaemolyticus was 0.125 mg/ml and 0.0625 mg/ml respectively. Consequentially, the amount of copper ion content zeolite required for bacterial inhibition was 6.25 mg/ml for V. campbellii and 5.00 mg/ml for V. parahaemo!yticus, which inherently were higher compared to the MICs value of silver ion content zeolite. The ability of the AgZ and CuZ to inhibit the biofilm formation was then studied by Crystal Violet Biofilm Inhibition Assay. Based on this assay, the percentage biofilm inhibition (BI) of AgZ and CuZ were obtained where, the CuZ gives 39.18% and 43.49% BI against V. campbellii and V parahaemolyticus correspondingly. Accordingly, AgZ shows 60.24% BI against IV, campbellii and 77.32% BI against V. parahaemolyticus. Therefore, due to its significant antibacterial performance, Ag and Cu? ion-exchanged zeolite has great potential to be used as an alternative to the organic antibiotics, especially in the aquaculture industry. 2020 Thesis NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/41687/1/24%20PAGES.pdf text en https://eprints.ums.edu.my/id/eprint/41687/2/FULLTEXT.pdf Nur Ariffah Waly (2020) Antibacterial properties of bentonite based zeolite a ion exchanged with silver and copper against marine bacterial pathogens. Masters thesis, Universiti Malaysia Sabah.
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
English
topic RM300-666 Drugs and their actions
spellingShingle RM300-666 Drugs and their actions
Nur Ariffah Waly
Antibacterial properties of bentonite based zeolite a ion exchanged with silver and copper against marine bacterial pathogens
description Development of aquaculture industry has been increasing rapidly around the world. However, this development is facing a great challenge due to bacterial disease outbreaks, leading to high mortality rates of the aquaculture population. Attempts to overcome this problem has resulted in a new greater challenge of antimicrobial resistance of bacteria due to broad usage of antibiotics. Therefore, the development of inorganic antibacterial agent, through the synthesis of bentonite based Zeolite A, further integrated with Ag and Cu? via ion exchange was hence proposed as an alternative to combat the bacteria. The bentonite clay as Si-Al source for zeolite synthesis was thermally transformed into an active phase at 100 ·C for 24 hours in the presence of concentrated hydrochloric acid, followed with the preparation of zeolite A mixture. Mixture of Zeolite A was prepared by using 2.5NaO: Al5Os: 1.5Si0,: 166H0 molar ratio. Hydrothermal synthesis of Zeolite A was carried out by aging process for 30 minutes followed by crystallization for 8 hours at 100 C and it was found that the optimum silica to alumina ratio and molarity of NaOH for the synthesis was 1.50 and 2.50 M respectively. Subsequently, the Zeolite A product underwent ion-exchange process with Ag and Cu?'and the metal content of ion-exchanged zeolite was determined by ICP-OES. The results obtained shows that pH and concentration of metal ion solution played important parts in ion-exchange process. The highest Cu? content was found on Zeolite A that was treated with 1.50 M of copper solution at pH 6 which is equal to 93.630 ± 1.114 (mg/g). Meanwhile, the highest Ag+ content was found on Zeolite A that was introduced to 1.50 M of silver solution at pH 6 which is equal to 190.511 ± 1.989 (mg/g). Antibacterial activity of the Ag (Ag?) and Cu?' (CuZ) ionexchanged zeolite A samples against V. campbellii and V. parahaemolyticus were evaluated by determining the Minimum Inhibition Concentration (MICs) using twofold serial dilutions of ion loaded zeolites in Tryptic Soy broth (TSB) supplemented with 2% of NaCl. The concentrations for AgZ and CuZ ranged from 0.125 mg/ml - 2.00 mg/ml and 5.00 mg/ml - 25.00 mg/ml respectively. The resulting results obtained shows that, the highest metal ion content zeolite A gives the lowest MICs value. The highest silver ion content in zeolite A needed to inhibit the growth of V. campbe/lii and V. parahaemolyticus was 0.125 mg/ml and 0.0625 mg/ml respectively. Consequentially, the amount of copper ion content zeolite required for bacterial inhibition was 6.25 mg/ml for V. campbellii and 5.00 mg/ml for V. parahaemo!yticus, which inherently were higher compared to the MICs value of silver ion content zeolite. The ability of the AgZ and CuZ to inhibit the biofilm formation was then studied by Crystal Violet Biofilm Inhibition Assay. Based on this assay, the percentage biofilm inhibition (BI) of AgZ and CuZ were obtained where, the CuZ gives 39.18% and 43.49% BI against V. campbellii and V parahaemolyticus correspondingly. Accordingly, AgZ shows 60.24% BI against IV, campbellii and 77.32% BI against V. parahaemolyticus. Therefore, due to its significant antibacterial performance, Ag and Cu? ion-exchanged zeolite has great potential to be used as an alternative to the organic antibiotics, especially in the aquaculture industry.
format Thesis
author Nur Ariffah Waly
author_facet Nur Ariffah Waly
author_sort Nur Ariffah Waly
title Antibacterial properties of bentonite based zeolite a ion exchanged with silver and copper against marine bacterial pathogens
title_short Antibacterial properties of bentonite based zeolite a ion exchanged with silver and copper against marine bacterial pathogens
title_full Antibacterial properties of bentonite based zeolite a ion exchanged with silver and copper against marine bacterial pathogens
title_fullStr Antibacterial properties of bentonite based zeolite a ion exchanged with silver and copper against marine bacterial pathogens
title_full_unstemmed Antibacterial properties of bentonite based zeolite a ion exchanged with silver and copper against marine bacterial pathogens
title_sort antibacterial properties of bentonite based zeolite a ion exchanged with silver and copper against marine bacterial pathogens
publishDate 2020
url https://eprints.ums.edu.my/id/eprint/41687/1/24%20PAGES.pdf
https://eprints.ums.edu.my/id/eprint/41687/2/FULLTEXT.pdf
https://eprints.ums.edu.my/id/eprint/41687/
_version_ 1817843840420151296
score 13.223943

Similar Items