Staff View: Exploring the potential of endophytic bacteria isolated from Sargassum sp.: Enhancing growth and non-specific immune response of cobia Rachycentron canadum (Linnaeus, 1766)

Exploring the potential of endophytic bacteria isolated from Sargassum sp.: Enhancing growth and non-specific immune response of cobia Rachycentron canadum (Linnaeus, 1766)

This study aimed to investigate the effects of adding endophytic bacterial isolates and Sargassum sp. algae extract to enhance the growth performance and non-specific immune response of cobia (Rachycentron canadum) fry. The research employed a Completely Randomized Design (CRD) experimental method w...

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Main Authors:	Fittrie M. Pratiwy, Muhammad I. Sumantri, Suryadi Saputra, Rosidah, Walim Lili, Iskandar Agung, Thallita N. Faddilah, Rafidah Othman
Format:	Article
Language:	English English
Published:	ResearchGate 2024
Subjects:	QR1-502 Microbiology SH20.3-191 Aquaculture
Online Access:	https://eprints.ums.edu.my/id/eprint/41568/1/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/41568/2/FULL%20TEXT.pdf https://eprints.ums.edu.my/id/eprint/41568/
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spelling	my.ums.eprints.415682024-10-24T04:17:34Z https://eprints.ums.edu.my/id/eprint/41568/ Exploring the potential of endophytic bacteria isolated from Sargassum sp.: Enhancing growth and non-specific immune response of cobia Rachycentron canadum (Linnaeus, 1766) Fittrie M. Pratiwy Muhammad I. Sumantri Suryadi Saputra Rosidah Walim Lili Iskandar Agung Thallita N. Faddilah Rafidah Othman QR1-502 Microbiology SH20.3-191 Aquaculture This study aimed to investigate the effects of adding endophytic bacterial isolates and Sargassum sp. algae extract to enhance the growth performance and non-specific immune response of cobia (Rachycentron canadum) fry. The research employed a Completely Randomized Design (CRD) experimental method with three treatments and five replications. The treatments consisted of: (A) commercial feed without any additives, (B) commercial feed supplemented with 15 mL of Sargassum sp. extract, and (C) commercial feed supplemented with 15 mL of endophytic bacterial isolates from Sargassum sp. at a concentration of 108 CFU mL-1 . The fish fry were reared for 30 days, followed by a 14-day challenge test with Vibrio alginolyticus bacteria at a density of 107 CFU mL-1 , using the intraperitoneal (IP) injection method. Parameters observed included absolute weight gain, absolute length gain, specific growth rate (SGR), feed conversion ratio (FCR), feed efficiency (FE), total erythrocyte count, total leukocyte count, leukocyte differential, clinical symptoms, survival rate, and water quality. Data on absolute weight gain, absolute length gain, SGR, FCR, FE, total erythrocyte count, total leukocyte count, and survival rate were analyzed using ANOVA with a 95% confidence level, followed by Duncan's post hoc test for significant differences. Leukocyte differentials, clinical symptoms, and water quality data were analyzed descriptively. The main findings revealed that the addition of endophytic bacterial isolates and Sargassum sp. algae extract could enhance the growth and non-specific immune response of kobia fry. Treatment C proved to be the most effective, as evidenced by the increased values of absolute weight gain (35.72 g), absolute length gain (11 cm), SGR (3.17%), FCR (1.19%), FE (84.13%), total erythrocyte count (2.89×106 mm-3 ), total leukocyte count (38.56×103 mm3 ), and leukocyte differential percentages (lymphocytes 82.2%, monocytes 4.4%, neutrophils 8.8%), as well as the fastest recovery time (7 days) after the challenge test, with the highest survival rate of 90.7%. ResearchGate 2024 Article NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/41568/1/ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/41568/2/FULL%20TEXT.pdf Fittrie M. Pratiwy and Muhammad I. Sumantri and Suryadi Saputra and Rosidah and Walim Lili and Iskandar Agung and Thallita N. Faddilah and Rafidah Othman (2024) Exploring the potential of endophytic bacteria isolated from Sargassum sp.: Enhancing growth and non-specific immune response of cobia Rachycentron canadum (Linnaeus, 1766). AACL Bioflux, 17 (4). pp. 1-18. ISSN 1844-8143
institution	Universiti Malaysia Sabah
building	UMS Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaysia Sabah
content_source	UMS Institutional Repository
url_provider	http://eprints.ums.edu.my/
language	English English
topic	QR1-502 Microbiology SH20.3-191 Aquaculture
spellingShingle	QR1-502 Microbiology SH20.3-191 Aquaculture Fittrie M. Pratiwy Muhammad I. Sumantri Suryadi Saputra Rosidah Walim Lili Iskandar Agung Thallita N. Faddilah Rafidah Othman Exploring the potential of endophytic bacteria isolated from Sargassum sp.: Enhancing growth and non-specific immune response of cobia Rachycentron canadum (Linnaeus, 1766)
description	This study aimed to investigate the effects of adding endophytic bacterial isolates and Sargassum sp. algae extract to enhance the growth performance and non-specific immune response of cobia (Rachycentron canadum) fry. The research employed a Completely Randomized Design (CRD) experimental method with three treatments and five replications. The treatments consisted of: (A) commercial feed without any additives, (B) commercial feed supplemented with 15 mL of Sargassum sp. extract, and (C) commercial feed supplemented with 15 mL of endophytic bacterial isolates from Sargassum sp. at a concentration of 108 CFU mL-1 . The fish fry were reared for 30 days, followed by a 14-day challenge test with Vibrio alginolyticus bacteria at a density of 107 CFU mL-1 , using the intraperitoneal (IP) injection method. Parameters observed included absolute weight gain, absolute length gain, specific growth rate (SGR), feed conversion ratio (FCR), feed efficiency (FE), total erythrocyte count, total leukocyte count, leukocyte differential, clinical symptoms, survival rate, and water quality. Data on absolute weight gain, absolute length gain, SGR, FCR, FE, total erythrocyte count, total leukocyte count, and survival rate were analyzed using ANOVA with a 95% confidence level, followed by Duncan's post hoc test for significant differences. Leukocyte differentials, clinical symptoms, and water quality data were analyzed descriptively. The main findings revealed that the addition of endophytic bacterial isolates and Sargassum sp. algae extract could enhance the growth and non-specific immune response of kobia fry. Treatment C proved to be the most effective, as evidenced by the increased values of absolute weight gain (35.72 g), absolute length gain (11 cm), SGR (3.17%), FCR (1.19%), FE (84.13%), total erythrocyte count (2.89×106 mm-3 ), total leukocyte count (38.56×103 mm3 ), and leukocyte differential percentages (lymphocytes 82.2%, monocytes 4.4%, neutrophils 8.8%), as well as the fastest recovery time (7 days) after the challenge test, with the highest survival rate of 90.7%.
format	Article
author	Fittrie M. Pratiwy Muhammad I. Sumantri Suryadi Saputra Rosidah Walim Lili Iskandar Agung Thallita N. Faddilah Rafidah Othman
author_facet	Fittrie M. Pratiwy Muhammad I. Sumantri Suryadi Saputra Rosidah Walim Lili Iskandar Agung Thallita N. Faddilah Rafidah Othman
author_sort	Fittrie M. Pratiwy
title	Exploring the potential of endophytic bacteria isolated from Sargassum sp.: Enhancing growth and non-specific immune response of cobia Rachycentron canadum (Linnaeus, 1766)
title_short	Exploring the potential of endophytic bacteria isolated from Sargassum sp.: Enhancing growth and non-specific immune response of cobia Rachycentron canadum (Linnaeus, 1766)
title_full	Exploring the potential of endophytic bacteria isolated from Sargassum sp.: Enhancing growth and non-specific immune response of cobia Rachycentron canadum (Linnaeus, 1766)
title_fullStr	Exploring the potential of endophytic bacteria isolated from Sargassum sp.: Enhancing growth and non-specific immune response of cobia Rachycentron canadum (Linnaeus, 1766)
title_full_unstemmed	Exploring the potential of endophytic bacteria isolated from Sargassum sp.: Enhancing growth and non-specific immune response of cobia Rachycentron canadum (Linnaeus, 1766)
title_sort	exploring the potential of endophytic bacteria isolated from sargassum sp.: enhancing growth and non-specific immune response of cobia rachycentron canadum (linnaeus, 1766)
publisher	ResearchGate
publishDate	2024
url	https://eprints.ums.edu.my/id/eprint/41568/1/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/41568/2/FULL%20TEXT.pdf https://eprints.ums.edu.my/id/eprint/41568/
_version_	1814049473950646272
score	13.211869

Exploring the potential of endophytic bacteria isolated from Sargassum sp.: Enhancing growth and non-specific immune response of cobia Rachycentron canadum (Linnaeus, 1766)

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