Analysis of Plasmodium knowlesi mitochondrial DNA aiming to identify molecular markers useful for epidemiological analysis of the zoonotic malaria in Sabah
Introduction: Although no indigenous human malaria cases have been recorded since 2018 in Malaysia, people in the country, especially those in the two states in Borneo, are still at the risk of zoonotic malaria caused by Plasmodium knowlesi. For better control of P. knowlesi malaria, epidemiological...
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Universiti Putra Malaysia
2023
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my.ums.eprints.415162024-10-22T06:32:22Z https://eprints.ums.edu.my/id/eprint/41516/ Analysis of Plasmodium knowlesi mitochondrial DNA aiming to identify molecular markers useful for epidemiological analysis of the zoonotic malaria in Sabah Shigeharu Sato Nelbon Giloi Naziatul Azlin Ibrahim Iloris Frederick Dony Kamruddin Ahmed Mohammad Saffree Jeffree RA643-645 Disease (Communicable and noninfectious) and public health RC109-216 Infectious and parasitic diseases Introduction: Although no indigenous human malaria cases have been recorded since 2018 in Malaysia, people in the country, especially those in the two states in Borneo, are still at the risk of zoonotic malaria caused by Plasmodium knowlesi. For better control of P. knowlesi malaria, epidemiological understanding of the disease is essential. Molecular markers in the mitochondrial DNA are expected to be useful, but characterisation of the parasite’s mitochondrial genome remains limited. Methods: From the total DNA extracted from P. knowlesi malaria blood samples collected in various districts in Sabah in 2020 and 2022, two overlapping DNA fragments covering a 2.3 kb region containing the parasite’s entire cox3 coding sequence were PCR amplified. Fragments of 12 different parasite strains were sequenced by direct Sanger sequencing and variations in the 2.3 kb region were identified by comparison with the reference sequence in the database. Results: Nine single nucleotide polymorphisms (SNPs)were identified in 10 strains investigated in this study. Seven of these SNPs were strain-specific, while remaining 2 were shared by 3 different strains each. In addition, one of the A clusters present in the reference sequence was extended in all the 12 strains investigated. Conclusion: The SNPs identified here will probably serve as useful molecular markers in epidemiological study of P. knowlesi in Sabah. The fact that 9 SNPs were identified in the 2.3 kb region of only 12 strains analysed proves that the mitochondrial DNA is a good source of molecular markers useful in P. knowlesi research. Universiti Putra Malaysia 2023-12 Article NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/41516/1/ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/41516/2/FULL%20TEXT.pdf Shigeharu Sato and Nelbon Giloi and Naziatul Azlin Ibrahim and Iloris Frederick Dony and Kamruddin Ahmed and Mohammad Saffree Jeffree (2023) Analysis of Plasmodium knowlesi mitochondrial DNA aiming to identify molecular markers useful for epidemiological analysis of the zoonotic malaria in Sabah. Malaysian Journal of Medicine and Health Sciences, 19. pp. 9-15. |
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English English |
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RA643-645 Disease (Communicable and noninfectious) and public health RC109-216 Infectious and parasitic diseases |
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RA643-645 Disease (Communicable and noninfectious) and public health RC109-216 Infectious and parasitic diseases Shigeharu Sato Nelbon Giloi Naziatul Azlin Ibrahim Iloris Frederick Dony Kamruddin Ahmed Mohammad Saffree Jeffree Analysis of Plasmodium knowlesi mitochondrial DNA aiming to identify molecular markers useful for epidemiological analysis of the zoonotic malaria in Sabah |
| description |
Introduction: Although no indigenous human malaria cases have been recorded since 2018 in Malaysia, people in the country, especially those in the two states in Borneo, are still at the risk of zoonotic malaria caused by Plasmodium knowlesi. For better control of P. knowlesi malaria, epidemiological understanding of the disease is essential. Molecular markers in the mitochondrial DNA are expected to be useful, but characterisation of the parasite’s mitochondrial genome remains limited. Methods: From the total DNA extracted from P. knowlesi malaria blood samples collected in various districts in Sabah in 2020 and 2022, two overlapping DNA fragments covering a 2.3 kb region containing the parasite’s entire cox3 coding sequence were PCR amplified. Fragments of 12 different parasite strains were sequenced by direct Sanger sequencing and variations in the 2.3 kb region were identified by comparison with the reference sequence in the database. Results: Nine single nucleotide polymorphisms (SNPs)were identified in 10 strains investigated in this study. Seven of these SNPs were strain-specific, while remaining 2 were shared by 3 different strains each. In addition, one of the A clusters present in the reference sequence was extended in all the 12 strains investigated. Conclusion: The SNPs identified here will probably serve as useful molecular markers in epidemiological study of P. knowlesi in Sabah. The fact that 9 SNPs were identified in the 2.3 kb region of only 12 strains analysed proves that the mitochondrial DNA is a good source of molecular markers useful in P. knowlesi research. |
| format |
Article |
| author |
Shigeharu Sato Nelbon Giloi Naziatul Azlin Ibrahim Iloris Frederick Dony Kamruddin Ahmed Mohammad Saffree Jeffree |
| author_facet |
Shigeharu Sato Nelbon Giloi Naziatul Azlin Ibrahim Iloris Frederick Dony Kamruddin Ahmed Mohammad Saffree Jeffree |
| author_sort |
Shigeharu Sato |
| title |
Analysis of Plasmodium knowlesi mitochondrial DNA aiming to identify molecular markers useful for epidemiological analysis of the zoonotic malaria in Sabah |
| title_short |
Analysis of Plasmodium knowlesi mitochondrial DNA aiming to identify molecular markers useful for epidemiological analysis of the zoonotic malaria in Sabah |
| title_full |
Analysis of Plasmodium knowlesi mitochondrial DNA aiming to identify molecular markers useful for epidemiological analysis of the zoonotic malaria in Sabah |
| title_fullStr |
Analysis of Plasmodium knowlesi mitochondrial DNA aiming to identify molecular markers useful for epidemiological analysis of the zoonotic malaria in Sabah |
| title_full_unstemmed |
Analysis of Plasmodium knowlesi mitochondrial DNA aiming to identify molecular markers useful for epidemiological analysis of the zoonotic malaria in Sabah |
| title_sort |
analysis of plasmodium knowlesi mitochondrial dna aiming to identify molecular markers useful for epidemiological analysis of the zoonotic malaria in sabah |
| publisher |
Universiti Putra Malaysia |
| publishDate |
2023 |
| url |
https://eprints.ums.edu.my/id/eprint/41516/1/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/41516/2/FULL%20TEXT.pdf https://eprints.ums.edu.my/id/eprint/41516/ |
| _version_ |
1814049467031093248 |
| score |
13.251813 |