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In silico evaluation of crystallisation potential for conserved hypothetical proteins from the antarctic bacterium, Pedobacter cryoconitis BG5

The genome of Pedobacter cryoconitis BG5 highlighted the presence of conserved hypothetical proteins (HPs) with unique adaptive characteristics. Analysing and annotating the functions of these proteins was indeed essential for determining their suitability for X-ray crystallographic structure determ...

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Main Authors: Makdi Masnoddin, Clemente Michael Wong Vui Ling, Nur Athirah Yusof
格式: Proceedings
语言:English
English
出版: Pusat e-pembelajaran, UMS 2022
主题:
Q1-295 General
QR1-74.5 General
在线阅读:https://eprints.ums.edu.my/id/eprint/41260/1/ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/41260/2/FULL%20TEXT.pdf
https://eprints.ums.edu.my/id/eprint/41260/
https://oer.ums.edu.my/handle/oer_source_files/2441
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spelling my.ums.eprints.412602024-10-10T08:07:58Z https://eprints.ums.edu.my/id/eprint/41260/ In silico evaluation of crystallisation potential for conserved hypothetical proteins from the antarctic bacterium, Pedobacter cryoconitis BG5 Makdi Masnoddin Clemente Michael Wong Vui Ling Nur Athirah Yusof Q1-295 General QR1-74.5 General The genome of Pedobacter cryoconitis BG5 highlighted the presence of conserved hypothetical proteins (HPs) with unique adaptive characteristics. Analysing and annotating the functions of these proteins was indeed essential for determining their suitability for X-ray crystallographic structure determination. The HPs were examined for the presence of transmembrane helices and signal peptides, and their structural and functional characteristics were analyzed using various bioinformatics tools and databases. The analysis revealed twelve conserved HPs with significant thermal stress response functions that met crystallisation criteria. Functional annotation showed that most of the proteins contain domains related to an enzyme or catalytic activity. Furthermore, the physicochemical properties of the shortlisted protein suggest a high probability of crystallisation based on the previously solved crystal structures of proteins with similar features. This early identification of protein eligibility for crystal screening has the potential to significantly reduce the time and cost involved in the structural determination of the unknown proteins in P. cryoconitis BG5. Pusat e-pembelajaran, UMS 2022 Proceedings PeerReviewed text en https://eprints.ums.edu.my/id/eprint/41260/1/ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/41260/2/FULL%20TEXT.pdf Makdi Masnoddin and Clemente Michael Wong Vui Ling and Nur Athirah Yusof (2022) In silico evaluation of crystallisation potential for conserved hypothetical proteins from the antarctic bacterium, Pedobacter cryoconitis BG5. https://oer.ums.edu.my/handle/oer_source_files/2441
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
English
topic Q1-295 General
QR1-74.5 General
spellingShingle Q1-295 General
QR1-74.5 General
Makdi Masnoddin
Clemente Michael Wong Vui Ling
Nur Athirah Yusof
In silico evaluation of crystallisation potential for conserved hypothetical proteins from the antarctic bacterium, Pedobacter cryoconitis BG5
description The genome of Pedobacter cryoconitis BG5 highlighted the presence of conserved hypothetical proteins (HPs) with unique adaptive characteristics. Analysing and annotating the functions of these proteins was indeed essential for determining their suitability for X-ray crystallographic structure determination. The HPs were examined for the presence of transmembrane helices and signal peptides, and their structural and functional characteristics were analyzed using various bioinformatics tools and databases. The analysis revealed twelve conserved HPs with significant thermal stress response functions that met crystallisation criteria. Functional annotation showed that most of the proteins contain domains related to an enzyme or catalytic activity. Furthermore, the physicochemical properties of the shortlisted protein suggest a high probability of crystallisation based on the previously solved crystal structures of proteins with similar features. This early identification of protein eligibility for crystal screening has the potential to significantly reduce the time and cost involved in the structural determination of the unknown proteins in P. cryoconitis BG5.
format Proceedings
author Makdi Masnoddin
Clemente Michael Wong Vui Ling
Nur Athirah Yusof
author_facet Makdi Masnoddin
Clemente Michael Wong Vui Ling
Nur Athirah Yusof
author_sort Makdi Masnoddin
title In silico evaluation of crystallisation potential for conserved hypothetical proteins from the antarctic bacterium, Pedobacter cryoconitis BG5
title_short In silico evaluation of crystallisation potential for conserved hypothetical proteins from the antarctic bacterium, Pedobacter cryoconitis BG5
title_full In silico evaluation of crystallisation potential for conserved hypothetical proteins from the antarctic bacterium, Pedobacter cryoconitis BG5
title_fullStr In silico evaluation of crystallisation potential for conserved hypothetical proteins from the antarctic bacterium, Pedobacter cryoconitis BG5
title_full_unstemmed In silico evaluation of crystallisation potential for conserved hypothetical proteins from the antarctic bacterium, Pedobacter cryoconitis BG5
title_sort in silico evaluation of crystallisation potential for conserved hypothetical proteins from the antarctic bacterium, pedobacter cryoconitis bg5
publisher Pusat e-pembelajaran, UMS
publishDate 2022
url https://eprints.ums.edu.my/id/eprint/41260/1/ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/41260/2/FULL%20TEXT.pdf
https://eprints.ums.edu.my/id/eprint/41260/
https://oer.ums.edu.my/handle/oer_source_files/2441
_version_ 1814049441322106880
score 13.251813

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