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Occurrence of iridoviruses and bacterial pathogens in bivalves, scavenging fish and trash fish in marine aquaculture facilities in Sabah, Malaysia

Study was carried out to determine the occurrence of iridovirus and bacterial pathogen in bivalves, scavenging fish and trash fish collected from marine aquaculture facilities in Sabah. Bivalves and scavenging fish were collected from Kota Marudu, Kuala Penyu and Tuaran over a period of year from Ma...

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Main Author: Suraini Lajimin
Format: Thesis
Language:English
English
Published: 2015
Subjects:
SH171-179 Diseases and adverse factors
Online Access:https://eprints.ums.edu.my/id/eprint/39469/1/24%20PAGES.pdf
https://eprints.ums.edu.my/id/eprint/39469/2/FULLTEXT.pdf
https://eprints.ums.edu.my/id/eprint/39469/
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spelling my.ums.eprints.394692024-08-09T00:16:42Z https://eprints.ums.edu.my/id/eprint/39469/ Occurrence of iridoviruses and bacterial pathogens in bivalves, scavenging fish and trash fish in marine aquaculture facilities in Sabah, Malaysia Suraini Lajimin SH171-179 Diseases and adverse factors Study was carried out to determine the occurrence of iridovirus and bacterial pathogen in bivalves, scavenging fish and trash fish collected from marine aquaculture facilities in Sabah. Bivalves and scavenging fish were collected from Kota Marudu, Kuala Penyu and Tuaran over a period of year from May 2012 to April 2013. Meanwhile, trash fish specimens were collected from fisheries landing stations Kudat, Semporna, Lahad Datu, Kuala Penyu and Kota Kinabalu. From the Polymerase Chain Reaction (PCR) analysis, specimens of bivalves and scavenging fish (n=2068) were detected positive for iridovirus (266), Photobacterium damselae (53), Vibrio harveyi (135), Vibrio alginolyticus (44) and Vibrio parahaemolyticus (147). Meanwhile, 35 of trash fish (n=221) specimens were also detected positive for iridovirus using PCR. The DNA of positive iridovirus samples was then characterized by DNA sequencing. From the DNA sequencing analysis, it was shown that the iridovirus found on bivalves, scavenging fish and trash fish are from the genus Megalocytivirus (Iridoviridae). Further strain divergence analysis suggested that the megaloctivirus from bivalves and scavenging fish are from the same strain that is the Infectious Spleen Kidney and Necrosis Virus (ISKNV) with the average percentage of nucleotide similarity of 98.77%. Similarly,the megalocytivirus from trash fish also showed the highest average percentage nucleotide similarity to ISKNV (99.40%), which suggested that the megalocytivirus in Sabah are from the strain of ISKNV. Finding of this study revealed that some of the bivalves and scavenging fish which are abundance in the aquaculture facilities particularly floating net cage, are carrier of iridovirus, Vibrio harveyi, Vibrio parahaemolyticus, Vibrio alginolyticus and Photobacterium damselae. These animals can become the hosts of viral and bacterial pathogens and possibly transmit them to cultured fish. Finding of this study also showed that the trash fish can become the carrier of iridovirus and may transmit it to cultured fish during feeding. Therefore, the study suggests that the aquaculture facilities should often be cleaned from biofouling organisms such as bivalves to prevent disease outbreak. Furthermore, the use of trash fish should be minimized for the similar reason. 2015 Thesis NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/39469/1/24%20PAGES.pdf text en https://eprints.ums.edu.my/id/eprint/39469/2/FULLTEXT.pdf Suraini Lajimin (2015) Occurrence of iridoviruses and bacterial pathogens in bivalves, scavenging fish and trash fish in marine aquaculture facilities in Sabah, Malaysia. Masters thesis, Universiti Malaysia Sabah.
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
English
topic SH171-179 Diseases and adverse factors
spellingShingle SH171-179 Diseases and adverse factors
Suraini Lajimin
Occurrence of iridoviruses and bacterial pathogens in bivalves, scavenging fish and trash fish in marine aquaculture facilities in Sabah, Malaysia
description Study was carried out to determine the occurrence of iridovirus and bacterial pathogen in bivalves, scavenging fish and trash fish collected from marine aquaculture facilities in Sabah. Bivalves and scavenging fish were collected from Kota Marudu, Kuala Penyu and Tuaran over a period of year from May 2012 to April 2013. Meanwhile, trash fish specimens were collected from fisheries landing stations Kudat, Semporna, Lahad Datu, Kuala Penyu and Kota Kinabalu. From the Polymerase Chain Reaction (PCR) analysis, specimens of bivalves and scavenging fish (n=2068) were detected positive for iridovirus (266), Photobacterium damselae (53), Vibrio harveyi (135), Vibrio alginolyticus (44) and Vibrio parahaemolyticus (147). Meanwhile, 35 of trash fish (n=221) specimens were also detected positive for iridovirus using PCR. The DNA of positive iridovirus samples was then characterized by DNA sequencing. From the DNA sequencing analysis, it was shown that the iridovirus found on bivalves, scavenging fish and trash fish are from the genus Megalocytivirus (Iridoviridae). Further strain divergence analysis suggested that the megaloctivirus from bivalves and scavenging fish are from the same strain that is the Infectious Spleen Kidney and Necrosis Virus (ISKNV) with the average percentage of nucleotide similarity of 98.77%. Similarly,the megalocytivirus from trash fish also showed the highest average percentage nucleotide similarity to ISKNV (99.40%), which suggested that the megalocytivirus in Sabah are from the strain of ISKNV. Finding of this study revealed that some of the bivalves and scavenging fish which are abundance in the aquaculture facilities particularly floating net cage, are carrier of iridovirus, Vibrio harveyi, Vibrio parahaemolyticus, Vibrio alginolyticus and Photobacterium damselae. These animals can become the hosts of viral and bacterial pathogens and possibly transmit them to cultured fish. Finding of this study also showed that the trash fish can become the carrier of iridovirus and may transmit it to cultured fish during feeding. Therefore, the study suggests that the aquaculture facilities should often be cleaned from biofouling organisms such as bivalves to prevent disease outbreak. Furthermore, the use of trash fish should be minimized for the similar reason.
format Thesis
author Suraini Lajimin
author_facet Suraini Lajimin
author_sort Suraini Lajimin
title Occurrence of iridoviruses and bacterial pathogens in bivalves, scavenging fish and trash fish in marine aquaculture facilities in Sabah, Malaysia
title_short Occurrence of iridoviruses and bacterial pathogens in bivalves, scavenging fish and trash fish in marine aquaculture facilities in Sabah, Malaysia
title_full Occurrence of iridoviruses and bacterial pathogens in bivalves, scavenging fish and trash fish in marine aquaculture facilities in Sabah, Malaysia
title_fullStr Occurrence of iridoviruses and bacterial pathogens in bivalves, scavenging fish and trash fish in marine aquaculture facilities in Sabah, Malaysia
title_full_unstemmed Occurrence of iridoviruses and bacterial pathogens in bivalves, scavenging fish and trash fish in marine aquaculture facilities in Sabah, Malaysia
title_sort occurrence of iridoviruses and bacterial pathogens in bivalves, scavenging fish and trash fish in marine aquaculture facilities in sabah, malaysia
publishDate 2015
url https://eprints.ums.edu.my/id/eprint/39469/1/24%20PAGES.pdf
https://eprints.ums.edu.my/id/eprint/39469/2/FULLTEXT.pdf
https://eprints.ums.edu.my/id/eprint/39469/
_version_ 1807050425310904320
score 13.211869

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