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Antioxidant and Anti-Adipogenic Activities of Momordica cochinchinensis (Lour). Spreng Fruit Extracts

Momordica cochinchinensis (Lour) Spreng, known as gac fruit, is rich in bioactive compounds like carotenoids (β-carotene, lycopene, and lutein). This study assessed the antioxidant, cytotoxic, and anti-adipogenic properties of gac fruit extracts (GFE) from different fractions (peel, pulp, aril), usi...

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Main Authors: Mohd Nazri Abdul Rahman, Amin Ismail, Azrina Azlan, Ahmad Fazli Abdul Aziz, Nor Hayati Muhammad
Format: Article
Language:English
English
Published: Fakulti Perubatan Dan Sains Kesihatan, Universiti Putra Malaysia 2024
Subjects:
SB354-402 Fruit and fruit culture
TS920-937 Chemical processing of wood
Online Access:https://eprints.ums.edu.my/id/eprint/39176/1/ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/39176/2/FULL%20TEXT.pdf
https://eprints.ums.edu.my/id/eprint/39176/
https://doi.org/10.47836/mjmhs.20.3.26
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spelling my.ums.eprints.391762024-07-15T04:11:24Z https://eprints.ums.edu.my/id/eprint/39176/ Antioxidant and Anti-Adipogenic Activities of Momordica cochinchinensis (Lour). Spreng Fruit Extracts Mohd Nazri Abdul Rahman Amin Ismail Azrina Azlan Ahmad Fazli Abdul Aziz Nor Hayati Muhammad SB354-402 Fruit and fruit culture TS920-937 Chemical processing of wood Momordica cochinchinensis (Lour) Spreng, known as gac fruit, is rich in bioactive compounds like carotenoids (β-carotene, lycopene, and lutein). This study assessed the antioxidant, cytotoxic, and anti-adipogenic properties of gac fruit extracts (GFE) from different fractions (peel, pulp, aril), using 3T3-L1 adipocytes. Method: Gac extracts’ DPPH radical scavenging was tested with 1000µg/mL dilutions. 3T3-L1 pre-adipocyte viability was measured via MTT assay. Differentiated adipocytes were treated (75, 150, 300 µg/mL) with GFE for 7 days. Inhibitory effects on adipogenesis and lipid accumulation were studied through Oil Red O staining. Triglyceride content was quantified and compared to controls. Results: IC50 values against DPPH radicals were 660µg/mL (peel), 560µg/ mL (pulp), and 820µg/mL (aril). 3T3-L1 cell viability was unaffected up to 200µg/mL. However, 200µg/mL GFE decreased viability, inhibiting growth. Gac extracts effectively reduced lipid accumulation and hindered cell differentiation dose-dependently. Pulp extract notably reduced intracellular triglycerides, surpassing aril and peel effects. Conclusion: Gac fruit extract fractions (peel, pulp, and aril) efficiently inhibited adipogenesis in 3T3-L1 cells, evidenced by lowered lipid accumulation, triglyceride content, and cell viability. This study highlights gac fruit extracts’ potential therapeutic use against obesity. Fakulti Perubatan Dan Sains Kesihatan, Universiti Putra Malaysia 2024 Article NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/39176/1/ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/39176/2/FULL%20TEXT.pdf Mohd Nazri Abdul Rahman and Amin Ismail and Azrina Azlan and Ahmad Fazli Abdul Aziz and Nor Hayati Muhammad (2024) Antioxidant and Anti-Adipogenic Activities of Momordica cochinchinensis (Lour). Spreng Fruit Extracts. Malaysian Journal of Medicine and Health Sciences, 20. pp. 193-202. https://doi.org/10.47836/mjmhs.20.3.26
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
English
topic SB354-402 Fruit and fruit culture
TS920-937 Chemical processing of wood
spellingShingle SB354-402 Fruit and fruit culture
TS920-937 Chemical processing of wood
Mohd Nazri Abdul Rahman
Amin Ismail
Azrina Azlan
Ahmad Fazli Abdul Aziz
Nor Hayati Muhammad
Antioxidant and Anti-Adipogenic Activities of Momordica cochinchinensis (Lour). Spreng Fruit Extracts
description Momordica cochinchinensis (Lour) Spreng, known as gac fruit, is rich in bioactive compounds like carotenoids (β-carotene, lycopene, and lutein). This study assessed the antioxidant, cytotoxic, and anti-adipogenic properties of gac fruit extracts (GFE) from different fractions (peel, pulp, aril), using 3T3-L1 adipocytes. Method: Gac extracts’ DPPH radical scavenging was tested with 1000µg/mL dilutions. 3T3-L1 pre-adipocyte viability was measured via MTT assay. Differentiated adipocytes were treated (75, 150, 300 µg/mL) with GFE for 7 days. Inhibitory effects on adipogenesis and lipid accumulation were studied through Oil Red O staining. Triglyceride content was quantified and compared to controls. Results: IC50 values against DPPH radicals were 660µg/mL (peel), 560µg/ mL (pulp), and 820µg/mL (aril). 3T3-L1 cell viability was unaffected up to 200µg/mL. However, 200µg/mL GFE decreased viability, inhibiting growth. Gac extracts effectively reduced lipid accumulation and hindered cell differentiation dose-dependently. Pulp extract notably reduced intracellular triglycerides, surpassing aril and peel effects. Conclusion: Gac fruit extract fractions (peel, pulp, and aril) efficiently inhibited adipogenesis in 3T3-L1 cells, evidenced by lowered lipid accumulation, triglyceride content, and cell viability. This study highlights gac fruit extracts’ potential therapeutic use against obesity.
format Article
author Mohd Nazri Abdul Rahman
Amin Ismail
Azrina Azlan
Ahmad Fazli Abdul Aziz
Nor Hayati Muhammad
author_facet Mohd Nazri Abdul Rahman
Amin Ismail
Azrina Azlan
Ahmad Fazli Abdul Aziz
Nor Hayati Muhammad
author_sort Mohd Nazri Abdul Rahman
title Antioxidant and Anti-Adipogenic Activities of Momordica cochinchinensis (Lour). Spreng Fruit Extracts
title_short Antioxidant and Anti-Adipogenic Activities of Momordica cochinchinensis (Lour). Spreng Fruit Extracts
title_full Antioxidant and Anti-Adipogenic Activities of Momordica cochinchinensis (Lour). Spreng Fruit Extracts
title_fullStr Antioxidant and Anti-Adipogenic Activities of Momordica cochinchinensis (Lour). Spreng Fruit Extracts
title_full_unstemmed Antioxidant and Anti-Adipogenic Activities of Momordica cochinchinensis (Lour). Spreng Fruit Extracts
title_sort antioxidant and anti-adipogenic activities of momordica cochinchinensis (lour). spreng fruit extracts
publisher Fakulti Perubatan Dan Sains Kesihatan, Universiti Putra Malaysia
publishDate 2024
url https://eprints.ums.edu.my/id/eprint/39176/1/ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/39176/2/FULL%20TEXT.pdf
https://eprints.ums.edu.my/id/eprint/39176/
https://doi.org/10.47836/mjmhs.20.3.26
_version_ 1805887924296744960
score 13.211869

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