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Green honey of Banggi Island: A preliminary anti-diabetic study on zebrafish model

Zebrafish is a developing vertebrate model with several advantages, including its small size, and high experimental efficiency. Malaysia exhibit one of the highest diabetes rates in the Western Pacific and incurring an annual cost of 600 million US dollars. The objective of the study is to determine...

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Main Authors:	Saeed ullah, Fahrul Huyop, Nurul Huda, Roswanira Abdul Wahab, Azzmer Azzar Abdul Hamid, Mohd Azrul Naim, Hajar Fauzan Ahmad, Amir Husni Mohd Shariff, Mohd Hamzah Mohd Nasir
Format:	Article
Language:	English English
Published:	Elsevier Ltd 2024
Subjects:	QL614-639.8 Fishes QP501-801 Animal biochemistry
Online Access:	https://eprints.ums.edu.my/id/eprint/39173/1/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/39173/2/FULL%20TEXT.pdf https://eprints.ums.edu.my/id/eprint/39173/ https://doi.org/10.1016/j.heliyon.2024.e26469
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spelling	my.ums.eprints.391732024-07-15T04:01:38Z https://eprints.ums.edu.my/id/eprint/39173/ Green honey of Banggi Island: A preliminary anti-diabetic study on zebrafish model Saeed ullah Fahrul Huyop Nurul Huda Roswanira Abdul Wahab Azzmer Azzar Abdul Hamid Mohd Azrul Naim Hajar Fauzan Ahmad Amir Husni Mohd Shariff Mohd Hamzah Mohd Nasir QL614-639.8 Fishes QP501-801 Animal biochemistry Zebrafish is a developing vertebrate model with several advantages, including its small size, and high experimental efficiency. Malaysia exhibit one of the highest diabetes rates in the Western Pacific and incurring an annual cost of 600 million US dollars. The objective of the study is to determine the antidiabetic properties of green honey (GH) using a zebrafish model. Adult zebrafish, aged 3–4 months, were subjected to overfeeding and treated with streptozotocin (STZ) through intraperitoneal injection (IP) on days 7 and 9. The study assessed the oral sucrose tolerance test (OSTT) and the anti-diabetic effects of green honey. The evaluation was conducted at three time points: 30, 60, and 120 min after treatment and sucrose administration. The study utilised a model with a sample size of 5. The study was performed in six groups. These groups are (1) Normal control (non-diabetic, no intervention), (2) Normal control + GH (non-diabetic, supplemented with GH 3 μl), (3) DM control (diabetic, no intervention), (4) DM Gp1 (diabetic, 3 μL GH), (5) DM Gp2 (diabetic, 6 μ L GH), (6) DM Acarbose (diabetic, treated with acarbose). Fasting blood glucose levels for non-diabetic (non-DM) and diabetic (DM) groups were evaluated before and after the 10 days of diabetic induction. DM groups (excess of food and two injections of STZ) have caused a significant increment in the fasting blood glucose to 11.55 mmol/l (p < 0.0001). Both GH treatments effectively decreased postprandial blood glucose levels and the area under the curve in the oral glucose tolerance test (OSTT). Based on these results, it is concluded that green honey could play a role in hyperglycemia management and show potential as a natural alternative to conventional diabetes therapy. The underlying mechanisms need to be clarified, and their potential use in human diabetes therapy needs to be investigated. Elsevier Ltd 2024 Article NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/39173/1/ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/39173/2/FULL%20TEXT.pdf Saeed ullah and Fahrul Huyop and Nurul Huda and Roswanira Abdul Wahab and Azzmer Azzar Abdul Hamid and Mohd Azrul Naim and Hajar Fauzan Ahmad and Amir Husni Mohd Shariff and Mohd Hamzah Mohd Nasir (2024) Green honey of Banggi Island: A preliminary anti-diabetic study on zebrafish model. Heliyon, 10. pp. 1-8. ISSN 2405-8440 https://doi.org/10.1016/j.heliyon.2024.e26469
institution	Universiti Malaysia Sabah
building	UMS Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaysia Sabah
content_source	UMS Institutional Repository
url_provider	http://eprints.ums.edu.my/
language	English English
topic	QL614-639.8 Fishes QP501-801 Animal biochemistry
spellingShingle	QL614-639.8 Fishes QP501-801 Animal biochemistry Saeed ullah Fahrul Huyop Nurul Huda Roswanira Abdul Wahab Azzmer Azzar Abdul Hamid Mohd Azrul Naim Hajar Fauzan Ahmad Amir Husni Mohd Shariff Mohd Hamzah Mohd Nasir Green honey of Banggi Island: A preliminary anti-diabetic study on zebrafish model
description	Zebrafish is a developing vertebrate model with several advantages, including its small size, and high experimental efficiency. Malaysia exhibit one of the highest diabetes rates in the Western Pacific and incurring an annual cost of 600 million US dollars. The objective of the study is to determine the antidiabetic properties of green honey (GH) using a zebrafish model. Adult zebrafish, aged 3–4 months, were subjected to overfeeding and treated with streptozotocin (STZ) through intraperitoneal injection (IP) on days 7 and 9. The study assessed the oral sucrose tolerance test (OSTT) and the anti-diabetic effects of green honey. The evaluation was conducted at three time points: 30, 60, and 120 min after treatment and sucrose administration. The study utilised a model with a sample size of 5. The study was performed in six groups. These groups are (1) Normal control (non-diabetic, no intervention), (2) Normal control + GH (non-diabetic, supplemented with GH 3 μl), (3) DM control (diabetic, no intervention), (4) DM Gp1 (diabetic, 3 μL GH), (5) DM Gp2 (diabetic, 6 μ L GH), (6) DM Acarbose (diabetic, treated with acarbose). Fasting blood glucose levels for non-diabetic (non-DM) and diabetic (DM) groups were evaluated before and after the 10 days of diabetic induction. DM groups (excess of food and two injections of STZ) have caused a significant increment in the fasting blood glucose to 11.55 mmol/l (p < 0.0001). Both GH treatments effectively decreased postprandial blood glucose levels and the area under the curve in the oral glucose tolerance test (OSTT). Based on these results, it is concluded that green honey could play a role in hyperglycemia management and show potential as a natural alternative to conventional diabetes therapy. The underlying mechanisms need to be clarified, and their potential use in human diabetes therapy needs to be investigated.
format	Article
author	Saeed ullah Fahrul Huyop Nurul Huda Roswanira Abdul Wahab Azzmer Azzar Abdul Hamid Mohd Azrul Naim Hajar Fauzan Ahmad Amir Husni Mohd Shariff Mohd Hamzah Mohd Nasir
author_facet	Saeed ullah Fahrul Huyop Nurul Huda Roswanira Abdul Wahab Azzmer Azzar Abdul Hamid Mohd Azrul Naim Hajar Fauzan Ahmad Amir Husni Mohd Shariff Mohd Hamzah Mohd Nasir
author_sort	Saeed ullah
title	Green honey of Banggi Island: A preliminary anti-diabetic study on zebrafish model
title_short	Green honey of Banggi Island: A preliminary anti-diabetic study on zebrafish model
title_full	Green honey of Banggi Island: A preliminary anti-diabetic study on zebrafish model
title_fullStr	Green honey of Banggi Island: A preliminary anti-diabetic study on zebrafish model
title_full_unstemmed	Green honey of Banggi Island: A preliminary anti-diabetic study on zebrafish model
title_sort	green honey of banggi island: a preliminary anti-diabetic study on zebrafish model
publisher	Elsevier Ltd
publishDate	2024
url	https://eprints.ums.edu.my/id/eprint/39173/1/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/39173/2/FULL%20TEXT.pdf https://eprints.ums.edu.my/id/eprint/39173/ https://doi.org/10.1016/j.heliyon.2024.e26469
_version_	1805887923836420096
score	13.211869

Green honey of Banggi Island: A preliminary anti-diabetic study on zebrafish model

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