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Bioalcohol production from gracilaria sp. fermentation using sasad

Marine biomass or macroalgae has been denoted as a potential feedstock due to its high carbohydrate content which can be a source for biofuel production through fermentation process. In this research, Graci/aria sp. from red macroalgae group was used as raw material to produce biofuel via fermentati...

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Main Author: Helvie Mansuit
Format: Thesis
Language:English
English
Published: 2018
Subjects:
QD241-441 Organic chemistry
Online Access:https://eprints.ums.edu.my/id/eprint/38911/1/24%20PAGES.pdf
https://eprints.ums.edu.my/id/eprint/38911/2/FULLTEXT.pdf
https://eprints.ums.edu.my/id/eprint/38911/
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spelling my.ums.eprints.389112024-06-21T07:15:54Z https://eprints.ums.edu.my/id/eprint/38911/ Bioalcohol production from gracilaria sp. fermentation using sasad Helvie Mansuit QD241-441 Organic chemistry Marine biomass or macroalgae has been denoted as a potential feedstock due to its high carbohydrate content which can be a source for biofuel production through fermentation process. In this research, Graci/aria sp. from red macroalgae group was used as raw material to produce biofuel via fermentation process. The carbohydrate content in Graci/aria sp. can be breakdown into simple sugar through hydrolysis process such as acid hydrolysis which was reported to be the most affordable and available method to be done. This process, however, may release inhibitors such as furfural and 5 HMF (hydroxymethyl furfural). Nonetheless, common ethanol producing agent such as Saccharomyces cerevisiae was reported unable to undergo a succesfully alcohol metabolic pathway in any substrate containing inhibitors. Thus, in this research Graci/aria sp .. was fermented using a local Borneo bioagent, Sasad. The effect of acid concentration variation from 0.2 M to 0.8 M in acid hydrolysis on glucose content and alcohol yield of Graci/aria sp. was described. Acid concentration of more than 0.8 M was not explored as it higher acid concentration of more than 1 M was believed to yield higher inhibitors such as levulinic acid that was formed from HMF. Then, the effect of acid hydrolysis duration ranging from 15 to 60 minutes on glucose content and alcohol yield was also measured. The alcohol content of Graci/aria sp. fermentation product was also analysed based on the effect of fermentation duration and pretreatment types. The highest glucose content before fermentation process was recorded from Graci/aria sp. hydrolyzed using 0.8 M sulphuric acid for 30 minutes yielded 18.25 g/L compared to unhydrolyzed and hot water treated Gracilariasp., 13.34 g/L and 14.22 g/L respectively. After fermentation, lowest glucose content showed by untreated Graci/aria sp. after 7 days fermentation; with only 7.57 g/L glucose left. Alcohol content analysis using GCMS showed that 1 - butanol - 3 - methyl or isoamyl can be detected in all fermentation product. Highest alcohol yield was obtained from untreated Graci/aria sp. after 28 days fermentation which was 0.486x1Q-4 g/g followed by hot water treated and acid hydrolyzed samples of 0.121x1Q-4 g/g and 0.030x10-4 g/g respectively. This study has showed that Sasad was able to ferment Graci/aria sp. under different hydrolysis type and it maybe possible to eliminate the pretreatment stage since highest alcohol yield was reported from the untreated sample. 2018 Thesis NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/38911/1/24%20PAGES.pdf text en https://eprints.ums.edu.my/id/eprint/38911/2/FULLTEXT.pdf Helvie Mansuit (2018) Bioalcohol production from gracilaria sp. fermentation using sasad. Masters thesis, Universiti Malaysia Sabah.
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
English
topic QD241-441 Organic chemistry
spellingShingle QD241-441 Organic chemistry
Helvie Mansuit
Bioalcohol production from gracilaria sp. fermentation using sasad
description Marine biomass or macroalgae has been denoted as a potential feedstock due to its high carbohydrate content which can be a source for biofuel production through fermentation process. In this research, Graci/aria sp. from red macroalgae group was used as raw material to produce biofuel via fermentation process. The carbohydrate content in Graci/aria sp. can be breakdown into simple sugar through hydrolysis process such as acid hydrolysis which was reported to be the most affordable and available method to be done. This process, however, may release inhibitors such as furfural and 5 HMF (hydroxymethyl furfural). Nonetheless, common ethanol producing agent such as Saccharomyces cerevisiae was reported unable to undergo a succesfully alcohol metabolic pathway in any substrate containing inhibitors. Thus, in this research Graci/aria sp .. was fermented using a local Borneo bioagent, Sasad. The effect of acid concentration variation from 0.2 M to 0.8 M in acid hydrolysis on glucose content and alcohol yield of Graci/aria sp. was described. Acid concentration of more than 0.8 M was not explored as it higher acid concentration of more than 1 M was believed to yield higher inhibitors such as levulinic acid that was formed from HMF. Then, the effect of acid hydrolysis duration ranging from 15 to 60 minutes on glucose content and alcohol yield was also measured. The alcohol content of Graci/aria sp. fermentation product was also analysed based on the effect of fermentation duration and pretreatment types. The highest glucose content before fermentation process was recorded from Graci/aria sp. hydrolyzed using 0.8 M sulphuric acid for 30 minutes yielded 18.25 g/L compared to unhydrolyzed and hot water treated Gracilariasp., 13.34 g/L and 14.22 g/L respectively. After fermentation, lowest glucose content showed by untreated Graci/aria sp. after 7 days fermentation; with only 7.57 g/L glucose left. Alcohol content analysis using GCMS showed that 1 - butanol - 3 - methyl or isoamyl can be detected in all fermentation product. Highest alcohol yield was obtained from untreated Graci/aria sp. after 28 days fermentation which was 0.486x1Q-4 g/g followed by hot water treated and acid hydrolyzed samples of 0.121x1Q-4 g/g and 0.030x10-4 g/g respectively. This study has showed that Sasad was able to ferment Graci/aria sp. under different hydrolysis type and it maybe possible to eliminate the pretreatment stage since highest alcohol yield was reported from the untreated sample.
format Thesis
author Helvie Mansuit
author_facet Helvie Mansuit
author_sort Helvie Mansuit
title Bioalcohol production from gracilaria sp. fermentation using sasad
title_short Bioalcohol production from gracilaria sp. fermentation using sasad
title_full Bioalcohol production from gracilaria sp. fermentation using sasad
title_fullStr Bioalcohol production from gracilaria sp. fermentation using sasad
title_full_unstemmed Bioalcohol production from gracilaria sp. fermentation using sasad
title_sort bioalcohol production from gracilaria sp. fermentation using sasad
publishDate 2018
url https://eprints.ums.edu.my/id/eprint/38911/1/24%20PAGES.pdf
https://eprints.ums.edu.my/id/eprint/38911/2/FULLTEXT.pdf
https://eprints.ums.edu.my/id/eprint/38911/
_version_ 1802978366259724288
score 13.211869

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