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Ethyl methanesulphonate (EMS)-Mediated mutagenesis induces genetic and morphological variations in eggplant (Solanum melongena L.)

Eggplant (Solanum melongena L.) is a vegetable that holds high economic and nutritional value and is renowned for its distinct shape, color and flavor. There has been a considerable focus on enhancing the genetic makeup of eggplant, with specific attention given to breeding for better agronomic char...

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Main Authors: Ranjita Subramaniam, Vijay Subbiah Kumar
Format: Article
Language:English
English
Published: ResearchGate 2023
Subjects:
QK1-989 Botany
QK495 Angiosperms
Online Access:https://eprints.ums.edu.my/id/eprint/38359/1/ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/38359/2/FULL%20TEXT.pdf
https://eprints.ums.edu.my/id/eprint/38359/
http://dx.doi.org/10.3390/ijpb14030053
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spelling my.ums.eprints.383592024-02-27T01:45:39Z https://eprints.ums.edu.my/id/eprint/38359/ Ethyl methanesulphonate (EMS)-Mediated mutagenesis induces genetic and morphological variations in eggplant (Solanum melongena L.) Ranjita Subramaniam Vijay Subbiah Kumar QK1-989 Botany QK495 Angiosperms Eggplant (Solanum melongena L.) is a vegetable that holds high economic and nutritional value and is renowned for its distinct shape, color and flavor. There has been a considerable focus on enhancing the genetic makeup of eggplant, with specific attention given to breeding for better agronomic characteristics. However, the crop suffers from a narrow genetic base. As part of the efforts to broaden the gene pool of eggplant, a chemical mutagenesis approach has been employed, aimed to generate eggplant genotypes with distinctive characteristics. Altogether 180 seeds of eggplant cultivar, Surya was treated with EMS at 0.7% v/v concentration. In the development of M2 generation, members of 16 M2 families were inspected for phenotypic variation. Notable variations were observed in traits such as plant height, leaf, flower and fruit morphologies. Furthermore, a subset of the mutants was selected to screen for any DNA alterations in a few targeted genes belonging to the Flowering Locus T/Terminal Flower 1 (FT/TFL1) gene family, via amplicon sequencing performed using Pacbio RSII. A mutant sample was discovered to carry a heterozygous mutation at the upstream region of the coding sequence of one of those particular genes. Taken together, the M2 families developed here represent valuable genetic resources that can be explored for gene functional analysis and future breeding programs of eggplant. ResearchGate 2023 Article NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/38359/1/ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/38359/2/FULL%20TEXT.pdf Ranjita Subramaniam and Vijay Subbiah Kumar (2023) Ethyl methanesulphonate (EMS)-Mediated mutagenesis induces genetic and morphological variations in eggplant (Solanum melongena L.). International Journal of Plant Biology, 14. pp. 1-15. http://dx.doi.org/10.3390/ijpb14030053
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
English
topic QK1-989 Botany
QK495 Angiosperms
spellingShingle QK1-989 Botany
QK495 Angiosperms
Ranjita Subramaniam
Vijay Subbiah Kumar
Ethyl methanesulphonate (EMS)-Mediated mutagenesis induces genetic and morphological variations in eggplant (Solanum melongena L.)
description Eggplant (Solanum melongena L.) is a vegetable that holds high economic and nutritional value and is renowned for its distinct shape, color and flavor. There has been a considerable focus on enhancing the genetic makeup of eggplant, with specific attention given to breeding for better agronomic characteristics. However, the crop suffers from a narrow genetic base. As part of the efforts to broaden the gene pool of eggplant, a chemical mutagenesis approach has been employed, aimed to generate eggplant genotypes with distinctive characteristics. Altogether 180 seeds of eggplant cultivar, Surya was treated with EMS at 0.7% v/v concentration. In the development of M2 generation, members of 16 M2 families were inspected for phenotypic variation. Notable variations were observed in traits such as plant height, leaf, flower and fruit morphologies. Furthermore, a subset of the mutants was selected to screen for any DNA alterations in a few targeted genes belonging to the Flowering Locus T/Terminal Flower 1 (FT/TFL1) gene family, via amplicon sequencing performed using Pacbio RSII. A mutant sample was discovered to carry a heterozygous mutation at the upstream region of the coding sequence of one of those particular genes. Taken together, the M2 families developed here represent valuable genetic resources that can be explored for gene functional analysis and future breeding programs of eggplant.
format Article
author Ranjita Subramaniam
Vijay Subbiah Kumar
author_facet Ranjita Subramaniam
Vijay Subbiah Kumar
author_sort Ranjita Subramaniam
title Ethyl methanesulphonate (EMS)-Mediated mutagenesis induces genetic and morphological variations in eggplant (Solanum melongena L.)
title_short Ethyl methanesulphonate (EMS)-Mediated mutagenesis induces genetic and morphological variations in eggplant (Solanum melongena L.)
title_full Ethyl methanesulphonate (EMS)-Mediated mutagenesis induces genetic and morphological variations in eggplant (Solanum melongena L.)
title_fullStr Ethyl methanesulphonate (EMS)-Mediated mutagenesis induces genetic and morphological variations in eggplant (Solanum melongena L.)
title_full_unstemmed Ethyl methanesulphonate (EMS)-Mediated mutagenesis induces genetic and morphological variations in eggplant (Solanum melongena L.)
title_sort ethyl methanesulphonate (ems)-mediated mutagenesis induces genetic and morphological variations in eggplant (solanum melongena l.)
publisher ResearchGate
publishDate 2023
url https://eprints.ums.edu.my/id/eprint/38359/1/ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/38359/2/FULL%20TEXT.pdf
https://eprints.ums.edu.my/id/eprint/38359/
http://dx.doi.org/10.3390/ijpb14030053
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score 13.211869

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