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Bacteria-sponge interaction : Existence of possible chemical correlation

The vast ocean has the reputation of having the greatest biodiversity. Therefore, represents an enormous resource for the discovery of chemotherapeutic agents. This particular investigation delves upon the study of bioactive compound from two marine sponges from coastal waters of Sabah, Amphimedon s...

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Main Author: Koh, Johleen Tse Boon
Format: Thesis
Language:English
English
Published: 2010
Subjects:
QL360-599.82 Invertebrates
Online Access:https://eprints.ums.edu.my/id/eprint/38031/1/24%20PAGES.pdf
https://eprints.ums.edu.my/id/eprint/38031/2/FULLTEXT.pdf
https://eprints.ums.edu.my/id/eprint/38031/
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spelling my.ums.eprints.380312024-01-26T07:20:19Z https://eprints.ums.edu.my/id/eprint/38031/ Bacteria-sponge interaction : Existence of possible chemical correlation Koh, Johleen Tse Boon QL360-599.82 Invertebrates The vast ocean has the reputation of having the greatest biodiversity. Therefore, represents an enormous resource for the discovery of chemotherapeutic agents. This particular investigation delves upon the study of bioactive compound from two marine sponges from coastal waters of Sabah, Amphimedon sp. and Xestospongia sp. and the most active sponge was then investigated for its relationship with its inherently available culturable bacteria in an effort to confirm the identity of the bioactive compound producer. All fractions except for fraction 1 and fraction 6 from the hexane crude extract from Amphimedon sp. showed antibacterial activity against 5 strains of marine environmental bacteria while all fractions except for fraction 1 from the 90% methanol crude extract showed antibacterial activity against 5 strains of marine environmental bacteria. Amphimedon sp. produced potent sterol as its bioactive compound, while Xestosponge sp produced aaptamine. Their identities were identified based _on extensive H 1-NMR and MS data. Spongebacteria relationship was carried out by isolating culturable bacteria from sponge tissues, and a total of 40 strains of bacteria were isolated from Amphimedon sp. but only one strain (AA1) was found to have bioactive activity. 16S rRNA regions of AAl were amplified using forward primer 27F (GAGTTTGATCCTGGCTCAG) while the reverse primer was JR1R (GACTACCAGGGTABCTAATC) and was compared with the top three matches provided by NCBI GenBank. Strain AA1 was found to be pro Bacillus sp. with 99% confidence and with e-value of zero. A mixture of fatty acids were isolated as active metabolite from AAl and upon gas chromatography (GC) analysis and antibacterial assay with corresponding commercial fatty acids. It was confirmed that hexadecenoate was the active compound in AAl. In conclusion, culturable bacteria were not responsible for the production of active compounds in Amphimedon sp. 2010 Thesis NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/38031/1/24%20PAGES.pdf text en https://eprints.ums.edu.my/id/eprint/38031/2/FULLTEXT.pdf Koh, Johleen Tse Boon (2010) Bacteria-sponge interaction : Existence of possible chemical correlation. Masters thesis, Universiti Malaysia Saba.
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
English
topic QL360-599.82 Invertebrates
spellingShingle QL360-599.82 Invertebrates
Koh, Johleen Tse Boon
Bacteria-sponge interaction : Existence of possible chemical correlation
description The vast ocean has the reputation of having the greatest biodiversity. Therefore, represents an enormous resource for the discovery of chemotherapeutic agents. This particular investigation delves upon the study of bioactive compound from two marine sponges from coastal waters of Sabah, Amphimedon sp. and Xestospongia sp. and the most active sponge was then investigated for its relationship with its inherently available culturable bacteria in an effort to confirm the identity of the bioactive compound producer. All fractions except for fraction 1 and fraction 6 from the hexane crude extract from Amphimedon sp. showed antibacterial activity against 5 strains of marine environmental bacteria while all fractions except for fraction 1 from the 90% methanol crude extract showed antibacterial activity against 5 strains of marine environmental bacteria. Amphimedon sp. produced potent sterol as its bioactive compound, while Xestosponge sp produced aaptamine. Their identities were identified based _on extensive H 1-NMR and MS data. Spongebacteria relationship was carried out by isolating culturable bacteria from sponge tissues, and a total of 40 strains of bacteria were isolated from Amphimedon sp. but only one strain (AA1) was found to have bioactive activity. 16S rRNA regions of AAl were amplified using forward primer 27F (GAGTTTGATCCTGGCTCAG) while the reverse primer was JR1R (GACTACCAGGGTABCTAATC) and was compared with the top three matches provided by NCBI GenBank. Strain AA1 was found to be pro Bacillus sp. with 99% confidence and with e-value of zero. A mixture of fatty acids were isolated as active metabolite from AAl and upon gas chromatography (GC) analysis and antibacterial assay with corresponding commercial fatty acids. It was confirmed that hexadecenoate was the active compound in AAl. In conclusion, culturable bacteria were not responsible for the production of active compounds in Amphimedon sp.
format Thesis
author Koh, Johleen Tse Boon
author_facet Koh, Johleen Tse Boon
author_sort Koh, Johleen Tse Boon
title Bacteria-sponge interaction : Existence of possible chemical correlation
title_short Bacteria-sponge interaction : Existence of possible chemical correlation
title_full Bacteria-sponge interaction : Existence of possible chemical correlation
title_fullStr Bacteria-sponge interaction : Existence of possible chemical correlation
title_full_unstemmed Bacteria-sponge interaction : Existence of possible chemical correlation
title_sort bacteria-sponge interaction : existence of possible chemical correlation
publishDate 2010
url https://eprints.ums.edu.my/id/eprint/38031/1/24%20PAGES.pdf
https://eprints.ums.edu.my/id/eprint/38031/2/FULLTEXT.pdf
https://eprints.ums.edu.my/id/eprint/38031/
_version_ 1789426013690658816
score 13.211869

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