Staff View: Synergistic effect of commercial mangosteen extract (Garcinia mangostana L.) and amoxicillin against methicillin-resistant Staphylococcus aureus (MRSA)

Synergistic effect of commercial mangosteen extract (Garcinia mangostana L.) and amoxicillin against methicillin-resistant Staphylococcus aureus (MRSA)

Antibiotic resistance occurs worldwide and has become a threat to humankind. Previous data have shown that antimicrobial resistance is a global issue demanding immediate resolution because it threatens the environment and society. The present work thus investigated the synergistic effects of commerc...

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Main Authors:	Permana, A., Nurzafirah Mazlan, Yusuf, E., Anua, S. M., Thung, T. Y., Baktir, A.
Format:	Article
Language:	English English
Published:	Universiti Putra Malaysia 2023
Subjects:	QK710-899 Plant physiology QR1-74.5 General
Online Access:	https://eprints.ums.edu.my/id/eprint/37996/1/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/37996/2/FULL%20TEXT.pdf https://eprints.ums.edu.my/id/eprint/37996/ https://doi.org/10.47836/ifrj.30.5.19
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spelling	my.ums.eprints.379962024-01-22T02:51:57Z https://eprints.ums.edu.my/id/eprint/37996/ Synergistic effect of commercial mangosteen extract (Garcinia mangostana L.) and amoxicillin against methicillin-resistant Staphylococcus aureus (MRSA) Permana, A. Nurzafirah Mazlan Yusuf, E. Anua, S. M. Thung, T. Y. Baktir, A. QK710-899 Plant physiology QR1-74.5 General Antibiotic resistance occurs worldwide and has become a threat to humankind. Previous data have shown that antimicrobial resistance is a global issue demanding immediate resolution because it threatens the environment and society. The present work thus investigated the synergistic effects of commercial Garcinia mangostana L. (GML) extract and amoxicillin on the growth of methicillin-resistant Staphylococcus aureus (MRSA) bacterial cells. A commercial GML extract was screened for phytochemical properties, and the presence of α-mangostin was detected using high-performance liquid chromatography (HPLC). The antibacterial activity of the commercial GML extract with amoxicillin was analysed by minimum inhibitory concentration (MIC) and checkerboard assays. The morphology ultrastructure of bacteria was observed using transmission electron microscopy (TEM), after treatment with commercial GML extract, either single or in combination with amoxicillin. The MICs of amoxicillin and commercial GML extract against MRSA bacteria were 250.00 and 137.50 μg/mL, respectively. The checkerboard assay showed synergistic activity in the combination of commercial GML extract (34.38 µg/mL) and amoxicillin (62.50 µg/mL) at fractional inhibitory concentration (FIC) index of < 0.5. Damage to the structure of bacteria occurred due to the commercial GML extract plus amoxicillin. It was observed that the loss of bacterial cell membranes led to an irregular bacterial structure. These findings provided evidence that the combination of commercial GML extract and amoxicillin could reverse bacterial resistance in order to determine the susceptibility of traditional drugs. Universiti Putra Malaysia 2023-10 Article NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/37996/1/ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/37996/2/FULL%20TEXT.pdf Permana, A. and Nurzafirah Mazlan and Yusuf, E. and Anua, S. M. and Thung, T. Y. and Baktir, A. (2023) Synergistic effect of commercial mangosteen extract (Garcinia mangostana L.) and amoxicillin against methicillin-resistant Staphylococcus aureus (MRSA). International Food Research Journal, 30. pp. 1313-1319. ISSN 1985-4668 https://doi.org/10.47836/ifrj.30.5.19
institution	Universiti Malaysia Sabah
building	UMS Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaysia Sabah
content_source	UMS Institutional Repository
url_provider	http://eprints.ums.edu.my/
language	English English
topic	QK710-899 Plant physiology QR1-74.5 General
spellingShingle	QK710-899 Plant physiology QR1-74.5 General Permana, A. Nurzafirah Mazlan Yusuf, E. Anua, S. M. Thung, T. Y. Baktir, A. Synergistic effect of commercial mangosteen extract (Garcinia mangostana L.) and amoxicillin against methicillin-resistant Staphylococcus aureus (MRSA)
description	Antibiotic resistance occurs worldwide and has become a threat to humankind. Previous data have shown that antimicrobial resistance is a global issue demanding immediate resolution because it threatens the environment and society. The present work thus investigated the synergistic effects of commercial Garcinia mangostana L. (GML) extract and amoxicillin on the growth of methicillin-resistant Staphylococcus aureus (MRSA) bacterial cells. A commercial GML extract was screened for phytochemical properties, and the presence of α-mangostin was detected using high-performance liquid chromatography (HPLC). The antibacterial activity of the commercial GML extract with amoxicillin was analysed by minimum inhibitory concentration (MIC) and checkerboard assays. The morphology ultrastructure of bacteria was observed using transmission electron microscopy (TEM), after treatment with commercial GML extract, either single or in combination with amoxicillin. The MICs of amoxicillin and commercial GML extract against MRSA bacteria were 250.00 and 137.50 μg/mL, respectively. The checkerboard assay showed synergistic activity in the combination of commercial GML extract (34.38 µg/mL) and amoxicillin (62.50 µg/mL) at fractional inhibitory concentration (FIC) index of < 0.5. Damage to the structure of bacteria occurred due to the commercial GML extract plus amoxicillin. It was observed that the loss of bacterial cell membranes led to an irregular bacterial structure. These findings provided evidence that the combination of commercial GML extract and amoxicillin could reverse bacterial resistance in order to determine the susceptibility of traditional drugs.
format	Article
author	Permana, A. Nurzafirah Mazlan Yusuf, E. Anua, S. M. Thung, T. Y. Baktir, A.
author_facet	Permana, A. Nurzafirah Mazlan Yusuf, E. Anua, S. M. Thung, T. Y. Baktir, A.
author_sort	Permana, A.
title	Synergistic effect of commercial mangosteen extract (Garcinia mangostana L.) and amoxicillin against methicillin-resistant Staphylococcus aureus (MRSA)
title_short	Synergistic effect of commercial mangosteen extract (Garcinia mangostana L.) and amoxicillin against methicillin-resistant Staphylococcus aureus (MRSA)
title_full	Synergistic effect of commercial mangosteen extract (Garcinia mangostana L.) and amoxicillin against methicillin-resistant Staphylococcus aureus (MRSA)
title_fullStr	Synergistic effect of commercial mangosteen extract (Garcinia mangostana L.) and amoxicillin against methicillin-resistant Staphylococcus aureus (MRSA)
title_full_unstemmed	Synergistic effect of commercial mangosteen extract (Garcinia mangostana L.) and amoxicillin against methicillin-resistant Staphylococcus aureus (MRSA)
title_sort	synergistic effect of commercial mangosteen extract (garcinia mangostana l.) and amoxicillin against methicillin-resistant staphylococcus aureus (mrsa)
publisher	Universiti Putra Malaysia
publishDate	2023
url	https://eprints.ums.edu.my/id/eprint/37996/1/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/37996/2/FULL%20TEXT.pdf https://eprints.ums.edu.my/id/eprint/37996/ https://doi.org/10.47836/ifrj.30.5.19
_version_	1789426009836093440
score	13.211869

Synergistic effect of commercial mangosteen extract (Garcinia mangostana L.) and amoxicillin against methicillin-resistant Staphylococcus aureus (MRSA)

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