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Recovery and application of chitosan from Sabah shrimp shell waste through deacetylation of chitin at different parameters

The comparison between head shell and body shell was carried out to determine the best option to be used in this study. Besides that, the deacetylation of chitin at different parameters were also investigated which including soaking frequency, temperature and time treatment factor, to recover chitos...

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Main Author: Flornica Alca Ahing
Format: Thesis
Language:English
English
Published: 2018
Subjects:
QP501-801 Animal biochemistry
Online Access:https://eprints.ums.edu.my/id/eprint/37453/1/FULLTEXT.pdf
https://eprints.ums.edu.my/id/eprint/37453/2/24%20PAGES.pdf
https://eprints.ums.edu.my/id/eprint/37453/
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spelling my.ums.eprints.374532023-10-02T07:25:15Z https://eprints.ums.edu.my/id/eprint/37453/ Recovery and application of chitosan from Sabah shrimp shell waste through deacetylation of chitin at different parameters Flornica Alca Ahing QP501-801 Animal biochemistry The comparison between head shell and body shell was carried out to determine the best option to be used in this study. Besides that, the deacetylation of chitin at different parameters were also investigated which including soaking frequency, temperature and time treatment factor, to recover chitosan. This study was performed by soaking frequency once and twice, temperatures at 50°C, 60°C, 70°C and 80°C, and time treatment 12, 24, 36 and 48 hours while the characterization of chitosan was determined in terms of moisture content, ash value, solubility and degree of deacetylation. Besides that, a preliminary investigation was carried out to study the effect of chitosan in tilapia fish feed towards growth and survival rate by using 1.00%, 2.00% and 3.00% chitosan. The results obtained in this study shows that the body shell was a better option to produce chitosan as it produces higher chitosan yield compare to head which were 13.35% and 7.27%, respectively. In the meantime, deacetylation process at room temperature for twice soaking gave a higher degree of deacetylation value and solubility compare to once which were 75.24% and 88.57%, while the solubility were 97.78% and 98.32%, respectively. Furthermore, the moisture content and ash value of the chitosan produced in this study ranged from 4.27% to 9.19% and 0.22% to 0.90%, respectively. In addition, deacetylation process carried out at temperature 70°C and time treatment 48 hour gave the highest solubility and degree of deacetylation which was 97 .00% and 90.96%, respectively. For the meantime, the application of chitosan-supplemented fish feed on tilapia suggests that 1.00% chitosan increased the highest fish weight with 142.80%, while the 3.00% chitosan increased the length with 48.74%. The survival rate of tilapia fish was also observed to be 100% when 1.00% and 2.00% chitosan were used as the feed, compared to control with 63.00% only. Thus, this study demonstrated temperature and time treatment factors play a crucial role in recovering a good solubility and degree of deacetylation quality of chitosan. Chitosan produced in this study was also high in quality with 90.96% of degree of deacetylation, which can be used in industry that need further investigation for different application. 2018 Thesis NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/37453/1/FULLTEXT.pdf text en https://eprints.ums.edu.my/id/eprint/37453/2/24%20PAGES.pdf Flornica Alca Ahing (2018) Recovery and application of chitosan from Sabah shrimp shell waste through deacetylation of chitin at different parameters. Masters thesis, Universiti Malaysia Sabah.
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
English
topic QP501-801 Animal biochemistry
spellingShingle QP501-801 Animal biochemistry
Flornica Alca Ahing
Recovery and application of chitosan from Sabah shrimp shell waste through deacetylation of chitin at different parameters
description The comparison between head shell and body shell was carried out to determine the best option to be used in this study. Besides that, the deacetylation of chitin at different parameters were also investigated which including soaking frequency, temperature and time treatment factor, to recover chitosan. This study was performed by soaking frequency once and twice, temperatures at 50°C, 60°C, 70°C and 80°C, and time treatment 12, 24, 36 and 48 hours while the characterization of chitosan was determined in terms of moisture content, ash value, solubility and degree of deacetylation. Besides that, a preliminary investigation was carried out to study the effect of chitosan in tilapia fish feed towards growth and survival rate by using 1.00%, 2.00% and 3.00% chitosan. The results obtained in this study shows that the body shell was a better option to produce chitosan as it produces higher chitosan yield compare to head which were 13.35% and 7.27%, respectively. In the meantime, deacetylation process at room temperature for twice soaking gave a higher degree of deacetylation value and solubility compare to once which were 75.24% and 88.57%, while the solubility were 97.78% and 98.32%, respectively. Furthermore, the moisture content and ash value of the chitosan produced in this study ranged from 4.27% to 9.19% and 0.22% to 0.90%, respectively. In addition, deacetylation process carried out at temperature 70°C and time treatment 48 hour gave the highest solubility and degree of deacetylation which was 97 .00% and 90.96%, respectively. For the meantime, the application of chitosan-supplemented fish feed on tilapia suggests that 1.00% chitosan increased the highest fish weight with 142.80%, while the 3.00% chitosan increased the length with 48.74%. The survival rate of tilapia fish was also observed to be 100% when 1.00% and 2.00% chitosan were used as the feed, compared to control with 63.00% only. Thus, this study demonstrated temperature and time treatment factors play a crucial role in recovering a good solubility and degree of deacetylation quality of chitosan. Chitosan produced in this study was also high in quality with 90.96% of degree of deacetylation, which can be used in industry that need further investigation for different application.
format Thesis
author Flornica Alca Ahing
author_facet Flornica Alca Ahing
author_sort Flornica Alca Ahing
title Recovery and application of chitosan from Sabah shrimp shell waste through deacetylation of chitin at different parameters
title_short Recovery and application of chitosan from Sabah shrimp shell waste through deacetylation of chitin at different parameters
title_full Recovery and application of chitosan from Sabah shrimp shell waste through deacetylation of chitin at different parameters
title_fullStr Recovery and application of chitosan from Sabah shrimp shell waste through deacetylation of chitin at different parameters
title_full_unstemmed Recovery and application of chitosan from Sabah shrimp shell waste through deacetylation of chitin at different parameters
title_sort recovery and application of chitosan from sabah shrimp shell waste through deacetylation of chitin at different parameters
publishDate 2018
url https://eprints.ums.edu.my/id/eprint/37453/1/FULLTEXT.pdf
https://eprints.ums.edu.my/id/eprint/37453/2/24%20PAGES.pdf
https://eprints.ums.edu.my/id/eprint/37453/
_version_ 1781705917190897664
score 13.211869

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