Cover Image

High-performance liquid chromatography (HPLC) analysis for flavonoids profiling of Napier grass herbal tea

Natural plant products are becoming more and more essential in helping to promote safe well-being worldwide. This leads to a substantial rise in the consumption of various herbal teas. The presence of beneficial bioactive ingredients such as flavonoids may be correlated with Napier grass herbal tea...

Full description

Saved in:
Bibliographic Details
Main Authors: Ng, K.S, Mohd Zin, Z., MohdMaidin, N., Mamat, H, Juhari, N.H., Zainol, M.K.
Format: Article
Language:English
English
Published: Rynnye Lyan Resources 2021
Subjects:
SB450.9-467.8 Gardens and gardening
TP315-360 Fuel
Online Access:https://eprints.ums.edu.my/id/eprint/36457/1/ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/36457/2/FULL%20TEXT.pdf
https://eprints.ums.edu.my/id/eprint/36457/
https://doi.org/10.26656/fr.2017.5(1).311
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.ums.eprints.36457
record_format eprints
spelling my.ums.eprints.364572023-08-17T04:32:30Z https://eprints.ums.edu.my/id/eprint/36457/ High-performance liquid chromatography (HPLC) analysis for flavonoids profiling of Napier grass herbal tea Ng, K.S Mohd Zin, Z. MohdMaidin, N. Mamat, H Juhari, N.H. Zainol, M.K. SB450.9-467.8 Gardens and gardening TP315-360 Fuel Natural plant products are becoming more and more essential in helping to promote safe well-being worldwide. This leads to a substantial rise in the consumption of various herbal teas. The presence of beneficial bioactive ingredients such as flavonoids may be correlated with Napier grass herbal tea having health benefits associated with their intake. Studies have shown that herbal teas have preferentially high antioxidant activity due to the presence of flavonoids in them. The purpose of this study was to identify the Napier grass herbal teas flavonoids prepared under different conditions. Napier grass herbal teas have been formulated using green tea and black tea processes, respectively. The tea samples were extracted in water (95°C, 30 mins) and 60% (v/v) aqueous methanol (30 mins), respectively. Approximately, 1% (v/v) aqueous acetic acid solution (solvent A) and acetonitrile (solvent B) were used as the mobile phase. The flow rate was adjusted to 0.7 mL/min, the column was thermostatically controlled at 28°C, and the injection column was kept at 20 µL. HPLC chromatograms were detected using a photodiode array UV detector at 272 nm. Gallic acid, P-coumaric acid, catechin, epigallocatechin gallate, quercetin, rutin, myricetin and kaempferol were found in both Napier grass water and methanolic extracts, respectively. The findings suggested that the HPLC techniques are ideal for the detection and identification of flavonoids in Napier grass teas. Rynnye Lyan Resources 2021-02 Article NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/36457/1/ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/36457/2/FULL%20TEXT.pdf Ng, K.S and Mohd Zin, Z. and MohdMaidin, N. and Mamat, H and Juhari, N.H. and Zainol, M.K. (2021) High-performance liquid chromatography (HPLC) analysis for flavonoids profiling of Napier grass herbal tea. Food Research, 5. 65 - 71. ISSN e-ISSN 2735 - 2226 https://doi.org/10.26656/fr.2017.5(1).311
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
English
topic SB450.9-467.8 Gardens and gardening
TP315-360 Fuel
spellingShingle SB450.9-467.8 Gardens and gardening
TP315-360 Fuel
Ng, K.S
Mohd Zin, Z.
MohdMaidin, N.
Mamat, H
Juhari, N.H.
Zainol, M.K.
High-performance liquid chromatography (HPLC) analysis for flavonoids profiling of Napier grass herbal tea
description Natural plant products are becoming more and more essential in helping to promote safe well-being worldwide. This leads to a substantial rise in the consumption of various herbal teas. The presence of beneficial bioactive ingredients such as flavonoids may be correlated with Napier grass herbal tea having health benefits associated with their intake. Studies have shown that herbal teas have preferentially high antioxidant activity due to the presence of flavonoids in them. The purpose of this study was to identify the Napier grass herbal teas flavonoids prepared under different conditions. Napier grass herbal teas have been formulated using green tea and black tea processes, respectively. The tea samples were extracted in water (95°C, 30 mins) and 60% (v/v) aqueous methanol (30 mins), respectively. Approximately, 1% (v/v) aqueous acetic acid solution (solvent A) and acetonitrile (solvent B) were used as the mobile phase. The flow rate was adjusted to 0.7 mL/min, the column was thermostatically controlled at 28°C, and the injection column was kept at 20 µL. HPLC chromatograms were detected using a photodiode array UV detector at 272 nm. Gallic acid, P-coumaric acid, catechin, epigallocatechin gallate, quercetin, rutin, myricetin and kaempferol were found in both Napier grass water and methanolic extracts, respectively. The findings suggested that the HPLC techniques are ideal for the detection and identification of flavonoids in Napier grass teas.
format Article
author Ng, K.S
Mohd Zin, Z.
MohdMaidin, N.
Mamat, H
Juhari, N.H.
Zainol, M.K.
author_facet Ng, K.S
Mohd Zin, Z.
MohdMaidin, N.
Mamat, H
Juhari, N.H.
Zainol, M.K.
author_sort Ng, K.S
title High-performance liquid chromatography (HPLC) analysis for flavonoids profiling of Napier grass herbal tea
title_short High-performance liquid chromatography (HPLC) analysis for flavonoids profiling of Napier grass herbal tea
title_full High-performance liquid chromatography (HPLC) analysis for flavonoids profiling of Napier grass herbal tea
title_fullStr High-performance liquid chromatography (HPLC) analysis for flavonoids profiling of Napier grass herbal tea
title_full_unstemmed High-performance liquid chromatography (HPLC) analysis for flavonoids profiling of Napier grass herbal tea
title_sort high-performance liquid chromatography (hplc) analysis for flavonoids profiling of napier grass herbal tea
publisher Rynnye Lyan Resources
publishDate 2021
url https://eprints.ums.edu.my/id/eprint/36457/1/ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/36457/2/FULL%20TEXT.pdf
https://eprints.ums.edu.my/id/eprint/36457/
https://doi.org/10.26656/fr.2017.5(1).311
_version_ 1775623864249745408
score 13.211869

Similar Items