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Optimization of an Innovative Hydrothermal Processing on Prebiotic Properties of Eucheuma denticulatum, a Tropical Red Seaweed

Seaweed is a sustainable source of marine oligosaccharides that potentially could be used as a prebiotic ingredient for functional food development. The study aims to optimize the oligosaccharide preparation through thermal hydrolysis of an under-utilized red seaweed, Eucheuma denticulatum. Response...

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Main Authors: Birdie Scott Padam, Chee Kiong Siew, Fook Yee Chye
Format: Article
Language:English
English
Published: Molecular Diversity Preservation International (MDPI) 2023
Subjects:
QR75-99.5 Bacteria
SH388.7-391.5 Algae culture
Online Access:https://eprints.ums.edu.my/id/eprint/35756/1/ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/35756/2/FULL%20TEXT.pdf
https://eprints.ums.edu.my/id/eprint/35756/
https://doi.org/10.3390/app13031517
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spelling my.ums.eprints.357562023-06-30T01:36:23Z https://eprints.ums.edu.my/id/eprint/35756/ Optimization of an Innovative Hydrothermal Processing on Prebiotic Properties of Eucheuma denticulatum, a Tropical Red Seaweed Birdie Scott Padam Chee Kiong Siew Fook Yee Chye QR75-99.5 Bacteria SH388.7-391.5 Algae culture Seaweed is a sustainable source of marine oligosaccharides that potentially could be used as a prebiotic ingredient for functional food development. The study aims to optimize the oligosaccharide preparation through thermal hydrolysis of an under-utilized red seaweed, Eucheuma denticulatum. Response surface methodology (RSM) applying Box–Behnken design (BBD) was used on three parameters including temperature (105–135 ◦C), hydrolysis time (15–35 min) and sulfuric acid concentration (0.05–0.2 M). Optimized fractions with good prebiotic activity were characterized using high-performance size-exclusion chromatography (HP-SEC) and Fourier transform infrared spectroscopy (FT-IR). Eucheuma denticulatum oligosaccharides fraction 1 (ED-F1) was shown to promote the growth of beneficial gut microbiota including Lactobacillus plantarum, L. casei, L. acidophilus, Bifidobacterium animalis and B. longum with the highest prebiotic activity score of 1.64 ± 0.17. The optimization studies showed that hydrolysis time was the most significant parameter for the oligosaccharides yield. Optimal hydrolysis conditions for ED-F1 were 120 ◦C, 21 min, 0.12 M H2SO4 with the highest yield achieved (11.15 g/100 g of dry weight). The molecular weight of ED-F1 was determined at 1025 Da while FT-IR analysis revealed the presence of sulfated oligosaccharides with similar characteristics of i-carrageenan. These findings signify the innovative method for the efficient production of seaweed derived prebiotic oligosaccharides, which could be a promising source of functional food ingredients for the development of health foods and beverages. Molecular Diversity Preservation International (MDPI) 2023 Article NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/35756/1/ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/35756/2/FULL%20TEXT.pdf Birdie Scott Padam and Chee Kiong Siew and Fook Yee Chye (2023) Optimization of an Innovative Hydrothermal Processing on Prebiotic Properties of Eucheuma denticulatum, a Tropical Red Seaweed. Applied Sciences, 13. pp. 1-15. https://doi.org/10.3390/app13031517
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
English
topic QR75-99.5 Bacteria
SH388.7-391.5 Algae culture
spellingShingle QR75-99.5 Bacteria
SH388.7-391.5 Algae culture
Birdie Scott Padam
Chee Kiong Siew
Fook Yee Chye
Optimization of an Innovative Hydrothermal Processing on Prebiotic Properties of Eucheuma denticulatum, a Tropical Red Seaweed
description Seaweed is a sustainable source of marine oligosaccharides that potentially could be used as a prebiotic ingredient for functional food development. The study aims to optimize the oligosaccharide preparation through thermal hydrolysis of an under-utilized red seaweed, Eucheuma denticulatum. Response surface methodology (RSM) applying Box–Behnken design (BBD) was used on three parameters including temperature (105–135 ◦C), hydrolysis time (15–35 min) and sulfuric acid concentration (0.05–0.2 M). Optimized fractions with good prebiotic activity were characterized using high-performance size-exclusion chromatography (HP-SEC) and Fourier transform infrared spectroscopy (FT-IR). Eucheuma denticulatum oligosaccharides fraction 1 (ED-F1) was shown to promote the growth of beneficial gut microbiota including Lactobacillus plantarum, L. casei, L. acidophilus, Bifidobacterium animalis and B. longum with the highest prebiotic activity score of 1.64 ± 0.17. The optimization studies showed that hydrolysis time was the most significant parameter for the oligosaccharides yield. Optimal hydrolysis conditions for ED-F1 were 120 ◦C, 21 min, 0.12 M H2SO4 with the highest yield achieved (11.15 g/100 g of dry weight). The molecular weight of ED-F1 was determined at 1025 Da while FT-IR analysis revealed the presence of sulfated oligosaccharides with similar characteristics of i-carrageenan. These findings signify the innovative method for the efficient production of seaweed derived prebiotic oligosaccharides, which could be a promising source of functional food ingredients for the development of health foods and beverages.
format Article
author Birdie Scott Padam
Chee Kiong Siew
Fook Yee Chye
author_facet Birdie Scott Padam
Chee Kiong Siew
Fook Yee Chye
author_sort Birdie Scott Padam
title Optimization of an Innovative Hydrothermal Processing on Prebiotic Properties of Eucheuma denticulatum, a Tropical Red Seaweed
title_short Optimization of an Innovative Hydrothermal Processing on Prebiotic Properties of Eucheuma denticulatum, a Tropical Red Seaweed
title_full Optimization of an Innovative Hydrothermal Processing on Prebiotic Properties of Eucheuma denticulatum, a Tropical Red Seaweed
title_fullStr Optimization of an Innovative Hydrothermal Processing on Prebiotic Properties of Eucheuma denticulatum, a Tropical Red Seaweed
title_full_unstemmed Optimization of an Innovative Hydrothermal Processing on Prebiotic Properties of Eucheuma denticulatum, a Tropical Red Seaweed
title_sort optimization of an innovative hydrothermal processing on prebiotic properties of eucheuma denticulatum, a tropical red seaweed
publisher Molecular Diversity Preservation International (MDPI)
publishDate 2023
url https://eprints.ums.edu.my/id/eprint/35756/1/ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/35756/2/FULL%20TEXT.pdf
https://eprints.ums.edu.my/id/eprint/35756/
https://doi.org/10.3390/app13031517
_version_ 1770552500475133952
score 13.211869

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