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Small Rna datasets of drug-susceptible Mycobacterium tuberculosis strains from Sabah, Malaysia

These datasets present a list of small RNAs from three drugsusceptible Mycobacterium tuberculosis strains isolated from Sabah, Malaysia. Sputum samples were obtained from three tuberculosis patients belonging to different districts. The bacteria were detected using GeneXpert MTB/RIF, isolated and cu...

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Main Authors: Mohd Iskandar Jumat, Jaeyres Jani, Zainal Arifin Mustapha, Kenneth Francis Rodrigues, Nornazirah Azizan, Armando Acosta, Maria E. Sarmiento, Kai Ling Chin
Format: Article
Language:English
English
Published: National Library of Medicine 2022
Subjects:
QK900-989 Plant ecology
QP501-801 Animal biochemistry
Online Access:https://eprints.ums.edu.my/id/eprint/35674/1/ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/35674/2/FULL%20TEXT.pdf
https://eprints.ums.edu.my/id/eprint/35674/
https://doi.org/10.1016/j.dib.2022.108795
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spelling my.ums.eprints.356742023-06-21T02:05:56Z https://eprints.ums.edu.my/id/eprint/35674/ Small Rna datasets of drug-susceptible Mycobacterium tuberculosis strains from Sabah, Malaysia Mohd Iskandar Jumat Jaeyres Jani Zainal Arifin Mustapha Kenneth Francis Rodrigues Nornazirah Azizan Armando Acosta Maria E. Sarmiento Kai Ling Chin QK900-989 Plant ecology QP501-801 Animal biochemistry These datasets present a list of small RNAs from three drugsusceptible Mycobacterium tuberculosis strains isolated from Sabah, Malaysia. Sputum samples were obtained from three tuberculosis patients belonging to different districts. The bacteria were detected using GeneXpert MTB/RIF, isolated and cultured in BACTECTM MGITTM 320, and tested for their drug susceptibility. Total RNAs were extracted, sequenced, and analyzed using bioinformatic tools to filter out small RNA present in the Mycobacterium tuberculosis strains. Small RNA sequencing generated total raw reads of 63,252,209, 63,636,812, and 61,148,224 and total trimmed reads (15-30 nucleotides) of 51,533,188, 53,520,197, and 51,363,772 for Mycobacterium tuberculosis strain SBH49, SBH149, and SBH372, respectively. The raw data were submitted to the Sequence Read Archive (SRA) database of the National Center for Biotechnology Information (NCBI) under the accession numbers of SRX16744291 (SBH49), SRX16744292 (SBH149), and SRX16744293 (SBH372). Small RNAs play important roles in cellular processes such as cell differentiation, cell signaling,development of resistance to antibiotics and immune response, and metabolism regulation. The small RNAs determined here could provide further insights into various cellular processes crucial for Mycobacterium tuberculosis survivability and a better understanding of their gene regulation which ultimately opens a new pathway for combating tuberculosis infection. National Library of Medicine 2022 Article NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/35674/1/ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/35674/2/FULL%20TEXT.pdf Mohd Iskandar Jumat and Jaeyres Jani and Zainal Arifin Mustapha and Kenneth Francis Rodrigues and Nornazirah Azizan and Armando Acosta and Maria E. Sarmiento and Kai Ling Chin (2022) Small Rna datasets of drug-susceptible Mycobacterium tuberculosis strains from Sabah, Malaysia. PubMed. pp. 1-6. https://doi.org/10.1016/j.dib.2022.108795
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
English
topic QK900-989 Plant ecology
QP501-801 Animal biochemistry
spellingShingle QK900-989 Plant ecology
QP501-801 Animal biochemistry
Mohd Iskandar Jumat
Jaeyres Jani
Zainal Arifin Mustapha
Kenneth Francis Rodrigues
Nornazirah Azizan
Armando Acosta
Maria E. Sarmiento
Kai Ling Chin
Small Rna datasets of drug-susceptible Mycobacterium tuberculosis strains from Sabah, Malaysia
description These datasets present a list of small RNAs from three drugsusceptible Mycobacterium tuberculosis strains isolated from Sabah, Malaysia. Sputum samples were obtained from three tuberculosis patients belonging to different districts. The bacteria were detected using GeneXpert MTB/RIF, isolated and cultured in BACTECTM MGITTM 320, and tested for their drug susceptibility. Total RNAs were extracted, sequenced, and analyzed using bioinformatic tools to filter out small RNA present in the Mycobacterium tuberculosis strains. Small RNA sequencing generated total raw reads of 63,252,209, 63,636,812, and 61,148,224 and total trimmed reads (15-30 nucleotides) of 51,533,188, 53,520,197, and 51,363,772 for Mycobacterium tuberculosis strain SBH49, SBH149, and SBH372, respectively. The raw data were submitted to the Sequence Read Archive (SRA) database of the National Center for Biotechnology Information (NCBI) under the accession numbers of SRX16744291 (SBH49), SRX16744292 (SBH149), and SRX16744293 (SBH372). Small RNAs play important roles in cellular processes such as cell differentiation, cell signaling,development of resistance to antibiotics and immune response, and metabolism regulation. The small RNAs determined here could provide further insights into various cellular processes crucial for Mycobacterium tuberculosis survivability and a better understanding of their gene regulation which ultimately opens a new pathway for combating tuberculosis infection.
format Article
author Mohd Iskandar Jumat
Jaeyres Jani
Zainal Arifin Mustapha
Kenneth Francis Rodrigues
Nornazirah Azizan
Armando Acosta
Maria E. Sarmiento
Kai Ling Chin
author_facet Mohd Iskandar Jumat
Jaeyres Jani
Zainal Arifin Mustapha
Kenneth Francis Rodrigues
Nornazirah Azizan
Armando Acosta
Maria E. Sarmiento
Kai Ling Chin
author_sort Mohd Iskandar Jumat
title Small Rna datasets of drug-susceptible Mycobacterium tuberculosis strains from Sabah, Malaysia
title_short Small Rna datasets of drug-susceptible Mycobacterium tuberculosis strains from Sabah, Malaysia
title_full Small Rna datasets of drug-susceptible Mycobacterium tuberculosis strains from Sabah, Malaysia
title_fullStr Small Rna datasets of drug-susceptible Mycobacterium tuberculosis strains from Sabah, Malaysia
title_full_unstemmed Small Rna datasets of drug-susceptible Mycobacterium tuberculosis strains from Sabah, Malaysia
title_sort small rna datasets of drug-susceptible mycobacterium tuberculosis strains from sabah, malaysia
publisher National Library of Medicine
publishDate 2022
url https://eprints.ums.edu.my/id/eprint/35674/1/ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/35674/2/FULL%20TEXT.pdf
https://eprints.ums.edu.my/id/eprint/35674/
https://doi.org/10.1016/j.dib.2022.108795
_version_ 1769843679744229376
score 13.211869

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