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Genetic Diversity Analysis of Ginger (Zingiber officinale Roscoe.) Genotypes Using RAPD Markers

Ginger (Zingiber officinale Roscoe.) is an important spice crop in Bangladesh as well as in all over the world for its economical and medicinal values. A measure of the existing genetic diversity is essential for planning a meaningful breeding strategy. Moreover, assessment and characterization of g...

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Main Authors: MS Mia, AK Patwary, L Hassan, MM Hasan, Md. Amirul Alam, MA Latif, M Monjurul Alam
Format: Article
Language:English
English
Published: Marsland Press / Zhengzhou University 2014
Subjects:
QK1-989 Botany
SB1-1110 Plant culture
Online Access:https://eprints.ums.edu.my/id/eprint/34920/1/Abstract.pdf
https://eprints.ums.edu.my/id/eprint/34920/2/Full%20text.pdf
https://eprints.ums.edu.my/id/eprint/34920/
http://www.lifesciencesite.com/lsj/life1108/012_23167life110814_90_94.pdf
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spelling my.ums.eprints.349202022-11-22T03:25:55Z https://eprints.ums.edu.my/id/eprint/34920/ Genetic Diversity Analysis of Ginger (Zingiber officinale Roscoe.) Genotypes Using RAPD Markers MS Mia AK Patwary L Hassan MM Hasan Md. Amirul Alam MA Latif M Monjurul Alam QK1-989 Botany SB1-1110 Plant culture Ginger (Zingiber officinale Roscoe.) is an important spice crop in Bangladesh as well as in all over the world for its economical and medicinal values. A measure of the existing genetic diversity is essential for planning a meaningful breeding strategy. Moreover, assessment and characterization of genetic diversity of the available germplasm is important to know the source of gene for particular trait of interest. The present investigation was undertaken to assess the genetic diversity among eight ginger genotypes using RAPD markers. A total of 16 distinct DNA fragments ranging from 100–1000 bp were amplified by using three selected primers of which 10 (62.50%) were polymorphic. BARI ada-1 was more homogenous than others and Syedpuri was found less homogenous showing the low intra-variety similarity value (75.56). the genotype ‘Syedpuri’ was found as more diversified from the viewpoint of lowest intra-variety similarity index value, highest gene diversity, proportion of polymorphic loci and highest level of genetic variation. The cluster analysis indicated that the eight genotypes were grouped into two major clusters. ‘Indian’ alone formed the first major cluster while the second major cluster had seven genotypes and was divided into two minor clusters. China and Sherpuri genotype pair were very close to each other with the lowest genetic distance (0.03). On the other hand, Indian and Syedpuri pair was more distant to each other with the highest genetic distance (0.55). RAPD analysis revealed a considerable level of polymorphism among the studied genotypes. The genetic variation thus detected has significance for ginger improvement programs. Marsland Press / Zhengzhou University 2014 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/34920/1/Abstract.pdf text en https://eprints.ums.edu.my/id/eprint/34920/2/Full%20text.pdf MS Mia and AK Patwary and L Hassan and MM Hasan and Md. Amirul Alam and MA Latif and M Monjurul Alam (2014) Genetic Diversity Analysis of Ginger (Zingiber officinale Roscoe.) Genotypes Using RAPD Markers. Life Science Journal, 11. pp. 90-94. ISSN 1097-8135 (P-ISSN) , 2372-613X (E-ISSN) http://www.lifesciencesite.com/lsj/life1108/012_23167life110814_90_94.pdf
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
English
topic QK1-989 Botany
SB1-1110 Plant culture
spellingShingle QK1-989 Botany
SB1-1110 Plant culture
MS Mia
AK Patwary
L Hassan
MM Hasan
Md. Amirul Alam
MA Latif
M Monjurul Alam
Genetic Diversity Analysis of Ginger (Zingiber officinale Roscoe.) Genotypes Using RAPD Markers
description Ginger (Zingiber officinale Roscoe.) is an important spice crop in Bangladesh as well as in all over the world for its economical and medicinal values. A measure of the existing genetic diversity is essential for planning a meaningful breeding strategy. Moreover, assessment and characterization of genetic diversity of the available germplasm is important to know the source of gene for particular trait of interest. The present investigation was undertaken to assess the genetic diversity among eight ginger genotypes using RAPD markers. A total of 16 distinct DNA fragments ranging from 100–1000 bp were amplified by using three selected primers of which 10 (62.50%) were polymorphic. BARI ada-1 was more homogenous than others and Syedpuri was found less homogenous showing the low intra-variety similarity value (75.56). the genotype ‘Syedpuri’ was found as more diversified from the viewpoint of lowest intra-variety similarity index value, highest gene diversity, proportion of polymorphic loci and highest level of genetic variation. The cluster analysis indicated that the eight genotypes were grouped into two major clusters. ‘Indian’ alone formed the first major cluster while the second major cluster had seven genotypes and was divided into two minor clusters. China and Sherpuri genotype pair were very close to each other with the lowest genetic distance (0.03). On the other hand, Indian and Syedpuri pair was more distant to each other with the highest genetic distance (0.55). RAPD analysis revealed a considerable level of polymorphism among the studied genotypes. The genetic variation thus detected has significance for ginger improvement programs.
format Article
author MS Mia
AK Patwary
L Hassan
MM Hasan
Md. Amirul Alam
MA Latif
M Monjurul Alam
author_facet MS Mia
AK Patwary
L Hassan
MM Hasan
Md. Amirul Alam
MA Latif
M Monjurul Alam
author_sort MS Mia
title Genetic Diversity Analysis of Ginger (Zingiber officinale Roscoe.) Genotypes Using RAPD Markers
title_short Genetic Diversity Analysis of Ginger (Zingiber officinale Roscoe.) Genotypes Using RAPD Markers
title_full Genetic Diversity Analysis of Ginger (Zingiber officinale Roscoe.) Genotypes Using RAPD Markers
title_fullStr Genetic Diversity Analysis of Ginger (Zingiber officinale Roscoe.) Genotypes Using RAPD Markers
title_full_unstemmed Genetic Diversity Analysis of Ginger (Zingiber officinale Roscoe.) Genotypes Using RAPD Markers
title_sort genetic diversity analysis of ginger (zingiber officinale roscoe.) genotypes using rapd markers
publisher Marsland Press / Zhengzhou University
publishDate 2014
url https://eprints.ums.edu.my/id/eprint/34920/1/Abstract.pdf
https://eprints.ums.edu.my/id/eprint/34920/2/Full%20text.pdf
https://eprints.ums.edu.my/id/eprint/34920/
http://www.lifesciencesite.com/lsj/life1108/012_23167life110814_90_94.pdf
_version_ 1760231359799885824
score 13.211869

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