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Activity of plant essential oils against antibiotic resistant Enterococcus faecalis isolated from diarrheic children

Activity of plant essential oils and their fractions was evaluated against characterized isolates of antibiotic resistant Enterococcus faecalis recovered from diarrheic children. The isolates were confirmed by polymerase chain reaction (PCR) targeting 16S rRNA gene amplification followed by nucleoti...

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Main Authors:	Tehreem Ali, Arslan Sarwar, Aftab Ahmad Anjum, Tahir Yaqub, Basit Zeshan, Muhammad Asad Ali, Mian Muhammad Khubaib Sattar
Format:	Article
Language:	English English
Published:	SILICONPLEX 2022
Subjects:	RJ101-103 Child health. Child health services SB1-1110 Plant culture
Online Access:	https://eprints.ums.edu.my/id/eprint/34801/1/Abstract.pdf https://eprints.ums.edu.my/id/eprint/34801/2/Full%20text.pdf https://eprints.ums.edu.my/id/eprint/34801/ https://www.pjps.pk/article-search/
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id	my.ums.eprints.34801
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spelling	my.ums.eprints.348012022-11-10T12:37:10Z https://eprints.ums.edu.my/id/eprint/34801/ Activity of plant essential oils against antibiotic resistant Enterococcus faecalis isolated from diarrheic children Tehreem Ali Arslan Sarwar Aftab Ahmad Anjum Tahir Yaqub Basit Zeshan Muhammad Asad Ali Mian Muhammad Khubaib Sattar RJ101-103 Child health. Child health services SB1-1110 Plant culture Activity of plant essential oils and their fractions was evaluated against characterized isolates of antibiotic resistant Enterococcus faecalis recovered from diarrheic children. The isolates were confirmed by polymerase chain reaction (PCR) targeting 16S rRNA gene amplification followed by nucleotide sequencing and accession numbers retrieved were MW349990.1, MW349859.1, MW332122.1, MW356805.1, MW349975.1, MW349988.1, MW356790.1, MW356244.1, MW341593.1 and MW332549.1. These isolates were screened for antibiotic susceptibility to a wide range of antibiotic groups and mean zone of inhibition (ZOI) of all antibiotics were recorded. Antibacterial activity of plant essential oils (n=05) was checked against three antibiotic resistant isolates of E. faecalis. Three plant essential oils having higher ZOI including Cinnamomum verum, Syzygium aromaticum and Nigella sativa were used against resistant E. faecalis isolates to determine minimum inhibitory concentration (MIC). The lowest MIC observed was of S. aromaticum (11.39±3.94 mg mL-1). The S. aromaticum n-hexane plus chloroform fraction displayed higher mean ZOI (16.67±2.51 mm), while the lowest MIC was of n-hexane oil fraction. Based upon gas chromatography-mass spectrometry (GC/MS) analysis, the most effective fatty acid was eugenic acid which is present in higher proportion in both fractions. These fractions of essential oils proved safe for the treatment of antibiotic resistant diarrheic cases of children caused by E. faecalis. SILICONPLEX 2022 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/34801/1/Abstract.pdf text en https://eprints.ums.edu.my/id/eprint/34801/2/Full%20text.pdf Tehreem Ali and Arslan Sarwar and Aftab Ahmad Anjum and Tahir Yaqub and Basit Zeshan and Muhammad Asad Ali and Mian Muhammad Khubaib Sattar (2022) Activity of plant essential oils against antibiotic resistant Enterococcus faecalis isolated from diarrheic children. Pakistan Journal of Pharmaceutical Sciences, 35. pp. 1-9. ISSN 1011-601X https://www.pjps.pk/article-search/
institution	Universiti Malaysia Sabah
building	UMS Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaysia Sabah
content_source	UMS Institutional Repository
url_provider	http://eprints.ums.edu.my/
language	English English
topic	RJ101-103 Child health. Child health services SB1-1110 Plant culture
spellingShingle	RJ101-103 Child health. Child health services SB1-1110 Plant culture Tehreem Ali Arslan Sarwar Aftab Ahmad Anjum Tahir Yaqub Basit Zeshan Muhammad Asad Ali Mian Muhammad Khubaib Sattar Activity of plant essential oils against antibiotic resistant Enterococcus faecalis isolated from diarrheic children
description	Activity of plant essential oils and their fractions was evaluated against characterized isolates of antibiotic resistant Enterococcus faecalis recovered from diarrheic children. The isolates were confirmed by polymerase chain reaction (PCR) targeting 16S rRNA gene amplification followed by nucleotide sequencing and accession numbers retrieved were MW349990.1, MW349859.1, MW332122.1, MW356805.1, MW349975.1, MW349988.1, MW356790.1, MW356244.1, MW341593.1 and MW332549.1. These isolates were screened for antibiotic susceptibility to a wide range of antibiotic groups and mean zone of inhibition (ZOI) of all antibiotics were recorded. Antibacterial activity of plant essential oils (n=05) was checked against three antibiotic resistant isolates of E. faecalis. Three plant essential oils having higher ZOI including Cinnamomum verum, Syzygium aromaticum and Nigella sativa were used against resistant E. faecalis isolates to determine minimum inhibitory concentration (MIC). The lowest MIC observed was of S. aromaticum (11.39±3.94 mg mL-1). The S. aromaticum n-hexane plus chloroform fraction displayed higher mean ZOI (16.67±2.51 mm), while the lowest MIC was of n-hexane oil fraction. Based upon gas chromatography-mass spectrometry (GC/MS) analysis, the most effective fatty acid was eugenic acid which is present in higher proportion in both fractions. These fractions of essential oils proved safe for the treatment of antibiotic resistant diarrheic cases of children caused by E. faecalis.
format	Article
author	Tehreem Ali Arslan Sarwar Aftab Ahmad Anjum Tahir Yaqub Basit Zeshan Muhammad Asad Ali Mian Muhammad Khubaib Sattar
author_facet	Tehreem Ali Arslan Sarwar Aftab Ahmad Anjum Tahir Yaqub Basit Zeshan Muhammad Asad Ali Mian Muhammad Khubaib Sattar
author_sort	Tehreem Ali
title	Activity of plant essential oils against antibiotic resistant Enterococcus faecalis isolated from diarrheic children
title_short	Activity of plant essential oils against antibiotic resistant Enterococcus faecalis isolated from diarrheic children
title_full	Activity of plant essential oils against antibiotic resistant Enterococcus faecalis isolated from diarrheic children
title_fullStr	Activity of plant essential oils against antibiotic resistant Enterococcus faecalis isolated from diarrheic children
title_full_unstemmed	Activity of plant essential oils against antibiotic resistant Enterococcus faecalis isolated from diarrheic children
title_sort	activity of plant essential oils against antibiotic resistant enterococcus faecalis isolated from diarrheic children
publisher	SILICONPLEX
publishDate	2022
url	https://eprints.ums.edu.my/id/eprint/34801/1/Abstract.pdf https://eprints.ums.edu.my/id/eprint/34801/2/Full%20text.pdf https://eprints.ums.edu.my/id/eprint/34801/ https://www.pjps.pk/article-search/
_version_	1760231343695855616
score	13.211869

Activity of plant essential oils against antibiotic resistant Enterococcus faecalis isolated from diarrheic children

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