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The transcriptional characteristics of NADC34-like PRRSV in porcine alveolar macrophages

The widespread and endemic circulation of porcine reproductive and respiratory syndrome virus (PRRSV) cause persistent financial losses to the swine industry worldwide. In 2017, NADC34-like PRRSV-2 emerged in northeastern China and spread rapidly. The dynamics analysis of immune perturbations associ...

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Main Authors: Peixin Wang, Xin Ma, Riteng Zhang, Yongxin Zhao, Ruochen Hu, Chen Luo, Basit Zeshan, Zengqi Yang, Li Qiu, Juan Wang, Haijin Liu, Yefei Zhou, Xinglong Wang
Format: Article
Language:English
English
Published: Lausanne : Frontiers Research Foundation 2022
Subjects:
SB1-1110 Plant culture
Online Access:https://eprints.ums.edu.my/id/eprint/34793/1/Abstract.pdf
https://eprints.ums.edu.my/id/eprint/34793/2/Full%20text.pdf
https://eprints.ums.edu.my/id/eprint/34793/
https://www.frontiersin.org/articles/10.3389/fmicb.2022.1022481/full
https://doi.org/10.3389/fmicb.2022.1022481
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spelling my.ums.eprints.347932022-11-10T12:22:44Z https://eprints.ums.edu.my/id/eprint/34793/ The transcriptional characteristics of NADC34-like PRRSV in porcine alveolar macrophages Peixin Wang Xin Ma Riteng Zhang Yongxin Zhao Ruochen Hu Chen Luo Basit Zeshan Zengqi Yang Li Qiu Juan Wang Haijin Liu Yefei Zhou Xinglong Wang SB1-1110 Plant culture The widespread and endemic circulation of porcine reproductive and respiratory syndrome virus (PRRSV) cause persistent financial losses to the swine industry worldwide. In 2017, NADC34-like PRRSV-2 emerged in northeastern China and spread rapidly. The dynamics analysis of immune perturbations associated with novel PRRSV lineage is still incomplete. This study performed a time-course transcriptome sequencing of NADC34-like PRRSV strain YC-2020-infected porcine alveolar macrophages (PAMs) and compared them with JXA1-infected PAMs. The results illustrated dramatic changes in the host’s differentially expressed genes (DEGs) presented at different timepoints after PRRSV infection, and the expression profile of YC-2020 group is distinct from that of JXA1 group. Functional enrichment analysis showed that the expression of many inflammatory cytokines was up-regulated following YC-2020 infection but at a significantly lower magnitude than JXA1 group, in line with the trends for most interferon-stimulated genes (ISGs) and their regulators. Meanwhile, numerous components of histocompatibility complex (MHC) class II and phagosome presented a stronger transcription suppression after the YC-2020 infection. All results imply that YC-2020 may induce milder inflammatory responses, weaker antiviral processes, and more severe disturbance of antigen processing and presentation compared with HP-PRRSV. Additionally, LAPTM4A, GLMP, and LITAF, which were selected from weighted gene co-expression network analysis (WGCNA), could significantly inhibit PRRSV proliferation. This study provides fundamental data for understanding the biological characteristics of NADC34-like PRRSV and new insights into PRRSV evolution and prevention. Lausanne : Frontiers Research Foundation 2022 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/34793/1/Abstract.pdf text en https://eprints.ums.edu.my/id/eprint/34793/2/Full%20text.pdf Peixin Wang and Xin Ma and Riteng Zhang and Yongxin Zhao and Ruochen Hu and Chen Luo and Basit Zeshan and Zengqi Yang and Li Qiu and Juan Wang and Haijin Liu and Yefei Zhou and Xinglong Wang (2022) The transcriptional characteristics of NADC34-like PRRSV in porcine alveolar macrophages. Frontiers in Microbiology. pp. 1-15. ISSN 1664-302X https://www.frontiersin.org/articles/10.3389/fmicb.2022.1022481/full https://doi.org/10.3389/fmicb.2022.1022481
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
English
topic SB1-1110 Plant culture
spellingShingle SB1-1110 Plant culture
Peixin Wang
Xin Ma
Riteng Zhang
Yongxin Zhao
Ruochen Hu
Chen Luo
Basit Zeshan
Zengqi Yang
Li Qiu
Juan Wang
Haijin Liu
Yefei Zhou
Xinglong Wang
The transcriptional characteristics of NADC34-like PRRSV in porcine alveolar macrophages
description The widespread and endemic circulation of porcine reproductive and respiratory syndrome virus (PRRSV) cause persistent financial losses to the swine industry worldwide. In 2017, NADC34-like PRRSV-2 emerged in northeastern China and spread rapidly. The dynamics analysis of immune perturbations associated with novel PRRSV lineage is still incomplete. This study performed a time-course transcriptome sequencing of NADC34-like PRRSV strain YC-2020-infected porcine alveolar macrophages (PAMs) and compared them with JXA1-infected PAMs. The results illustrated dramatic changes in the host’s differentially expressed genes (DEGs) presented at different timepoints after PRRSV infection, and the expression profile of YC-2020 group is distinct from that of JXA1 group. Functional enrichment analysis showed that the expression of many inflammatory cytokines was up-regulated following YC-2020 infection but at a significantly lower magnitude than JXA1 group, in line with the trends for most interferon-stimulated genes (ISGs) and their regulators. Meanwhile, numerous components of histocompatibility complex (MHC) class II and phagosome presented a stronger transcription suppression after the YC-2020 infection. All results imply that YC-2020 may induce milder inflammatory responses, weaker antiviral processes, and more severe disturbance of antigen processing and presentation compared with HP-PRRSV. Additionally, LAPTM4A, GLMP, and LITAF, which were selected from weighted gene co-expression network analysis (WGCNA), could significantly inhibit PRRSV proliferation. This study provides fundamental data for understanding the biological characteristics of NADC34-like PRRSV and new insights into PRRSV evolution and prevention.
format Article
author Peixin Wang
Xin Ma
Riteng Zhang
Yongxin Zhao
Ruochen Hu
Chen Luo
Basit Zeshan
Zengqi Yang
Li Qiu
Juan Wang
Haijin Liu
Yefei Zhou
Xinglong Wang
author_facet Peixin Wang
Xin Ma
Riteng Zhang
Yongxin Zhao
Ruochen Hu
Chen Luo
Basit Zeshan
Zengqi Yang
Li Qiu
Juan Wang
Haijin Liu
Yefei Zhou
Xinglong Wang
author_sort Peixin Wang
title The transcriptional characteristics of NADC34-like PRRSV in porcine alveolar macrophages
title_short The transcriptional characteristics of NADC34-like PRRSV in porcine alveolar macrophages
title_full The transcriptional characteristics of NADC34-like PRRSV in porcine alveolar macrophages
title_fullStr The transcriptional characteristics of NADC34-like PRRSV in porcine alveolar macrophages
title_full_unstemmed The transcriptional characteristics of NADC34-like PRRSV in porcine alveolar macrophages
title_sort transcriptional characteristics of nadc34-like prrsv in porcine alveolar macrophages
publisher Lausanne : Frontiers Research Foundation
publishDate 2022
url https://eprints.ums.edu.my/id/eprint/34793/1/Abstract.pdf
https://eprints.ums.edu.my/id/eprint/34793/2/Full%20text.pdf
https://eprints.ums.edu.my/id/eprint/34793/
https://www.frontiersin.org/articles/10.3389/fmicb.2022.1022481/full
https://doi.org/10.3389/fmicb.2022.1022481
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score 13.211869

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