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MDMA-Induced BV2 Microglial Cell Activation in Vitro

Introduction: 3,4-Methylenedioxymethamphetamine (MDMA) is a type of psychostimulant drug that induces neurotoxicity. Even though several psychostimulant substances activate microglia, little is known about MDMA's effects on these cells, and evidence of MDMA-induced microglial activation is equi...

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Main Authors: Nasir Mohamad, Nor Suliana Mustafa, Nor Hidayah Abu Bakar, Ramli Musa, Liyana Hazwani Mohd Adnan, Nor Zidah Ahmad, Mohd Nazri Mohd Daud, Zulfa Izza Mohamed Hashim
Format: Article
Language:English
English
Published: Shahid Beheshti University of Medical Sciences 2022
Subjects:
RC321-571 Neurosciences. Biological psychiatry. Neuropsychiatry
Online Access:https://eprints.ums.edu.my/id/eprint/34781/2/FULL%20TEXT.pdf
https://eprints.ums.edu.my/id/eprint/34781/3/ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/34781/
https://journals.sbmu.ac.ir/jcma/article/view/38360/29071
https://doi.org/10.22037/jcma.v7i3.38360
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spelling my.ums.eprints.347812022-11-10T11:58:26Z https://eprints.ums.edu.my/id/eprint/34781/ MDMA-Induced BV2 Microglial Cell Activation in Vitro Nasir Mohamad Nor Suliana Mustafa Nor Hidayah Abu Bakar Ramli Musa Liyana Hazwani Mohd Adnan Nor Zidah Ahmad Mohd Nazri Mohd Daud Zulfa Izza Mohamed Hashim RC321-571 Neurosciences. Biological psychiatry. Neuropsychiatry Introduction: 3,4-Methylenedioxymethamphetamine (MDMA) is a type of psychostimulant drug that induces neurotoxicity. Even though several psychostimulant substances activate microglia, little is known about MDMA's effects on these cells, and evidence of MDMA-induced microglial activation is equivocal. Material and Methods: This study employed a murine microglial cell line, BV2, to examine the effects of MDMA on the microglia morphological changes and the survival of microglia in vitro. MDMA was incorporated into the media at the time of plating and cell number and levels of mitochondrial dehydrogenase activity (MTT) were determined in vitro. The level of pro-inflammatory cytokine TNF-α was also determined. Result: Treatment of BV2 cells with MDMA resulted in morphological changes, reduced cell viability after 24h incubation with the inhibitory concentration (IC50) value of 243.6 µg/mL, and increase TNF-α level in a dose-dependent manner. Conclusion: These findings proposed that MDMA could induce BV2 microglial cell activation in vitro and suggested that it has an important role in the development of MDMA use disorder. Shahid Beheshti University of Medical Sciences 2022 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/34781/2/FULL%20TEXT.pdf text en https://eprints.ums.edu.my/id/eprint/34781/3/ABSTRACT.pdf Nasir Mohamad and Nor Suliana Mustafa and Nor Hidayah Abu Bakar and Ramli Musa and Liyana Hazwani Mohd Adnan and Nor Zidah Ahmad and Mohd Nazri Mohd Daud and Zulfa Izza Mohamed Hashim (2022) MDMA-Induced BV2 Microglial Cell Activation in Vitro. Journal of Cellular and Molecular Anesthesia, 7 (3). pp. 139-145. ISSN 2538-2462 (P-ISSN) , 2476-5120 (E-ISSN) https://journals.sbmu.ac.ir/jcma/article/view/38360/29071 https://doi.org/10.22037/jcma.v7i3.38360
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
English
topic RC321-571 Neurosciences. Biological psychiatry. Neuropsychiatry
spellingShingle RC321-571 Neurosciences. Biological psychiatry. Neuropsychiatry
Nasir Mohamad
Nor Suliana Mustafa
Nor Hidayah Abu Bakar
Ramli Musa
Liyana Hazwani Mohd Adnan
Nor Zidah Ahmad
Mohd Nazri Mohd Daud
Zulfa Izza Mohamed Hashim
MDMA-Induced BV2 Microglial Cell Activation in Vitro
description Introduction: 3,4-Methylenedioxymethamphetamine (MDMA) is a type of psychostimulant drug that induces neurotoxicity. Even though several psychostimulant substances activate microglia, little is known about MDMA's effects on these cells, and evidence of MDMA-induced microglial activation is equivocal. Material and Methods: This study employed a murine microglial cell line, BV2, to examine the effects of MDMA on the microglia morphological changes and the survival of microglia in vitro. MDMA was incorporated into the media at the time of plating and cell number and levels of mitochondrial dehydrogenase activity (MTT) were determined in vitro. The level of pro-inflammatory cytokine TNF-α was also determined. Result: Treatment of BV2 cells with MDMA resulted in morphological changes, reduced cell viability after 24h incubation with the inhibitory concentration (IC50) value of 243.6 µg/mL, and increase TNF-α level in a dose-dependent manner. Conclusion: These findings proposed that MDMA could induce BV2 microglial cell activation in vitro and suggested that it has an important role in the development of MDMA use disorder.
format Article
author Nasir Mohamad
Nor Suliana Mustafa
Nor Hidayah Abu Bakar
Ramli Musa
Liyana Hazwani Mohd Adnan
Nor Zidah Ahmad
Mohd Nazri Mohd Daud
Zulfa Izza Mohamed Hashim
author_facet Nasir Mohamad
Nor Suliana Mustafa
Nor Hidayah Abu Bakar
Ramli Musa
Liyana Hazwani Mohd Adnan
Nor Zidah Ahmad
Mohd Nazri Mohd Daud
Zulfa Izza Mohamed Hashim
author_sort Nasir Mohamad
title MDMA-Induced BV2 Microglial Cell Activation in Vitro
title_short MDMA-Induced BV2 Microglial Cell Activation in Vitro
title_full MDMA-Induced BV2 Microglial Cell Activation in Vitro
title_fullStr MDMA-Induced BV2 Microglial Cell Activation in Vitro
title_full_unstemmed MDMA-Induced BV2 Microglial Cell Activation in Vitro
title_sort mdma-induced bv2 microglial cell activation in vitro
publisher Shahid Beheshti University of Medical Sciences
publishDate 2022
url https://eprints.ums.edu.my/id/eprint/34781/2/FULL%20TEXT.pdf
https://eprints.ums.edu.my/id/eprint/34781/3/ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/34781/
https://journals.sbmu.ac.ir/jcma/article/view/38360/29071
https://doi.org/10.22037/jcma.v7i3.38360
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score 13.211869

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