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Risk association, linkage disequilibrium, and haplotype analyses of ß-like globin gene polymorphisms with malaria risk in the Sabah population of Malaysian Borneo

Single nucleotide polymorphisms (SNPs) in the β-like globin gene of the human hosts to the risk of malaria are unclear. Therefore, this study investigates these associations in the Sabah population, with a high incidence of malaria cases. In brief, DNA was extracted from 188 post-diagnostic blood sa...

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Main Authors: Chong, Eric Tzyy Jiann, Goh, Lucky Poh Wah, Yap, Ho Jin, Yong, Eric Wei Choong, Lee, Ping Chin
Format: Article
Language:English
English
Published: Multidisciplinary Digital Publishing Institute (MDPI) 2022
Subjects:
TP248.13-248.65 Biotechnology
Online Access:https://eprints.ums.edu.my/id/eprint/34698/1/FULL%20TEXT.pdf
https://eprints.ums.edu.my/id/eprint/34698/2/ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/34698/
https://www.mdpi.com/2073-4425/13/7/1229/htm
https://doi.org/10.3390/genes13071229
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spelling my.ums.eprints.346982022-11-08T00:37:03Z https://eprints.ums.edu.my/id/eprint/34698/ Risk association, linkage disequilibrium, and haplotype analyses of ß-like globin gene polymorphisms with malaria risk in the Sabah population of Malaysian Borneo Chong, Eric Tzyy Jiann Goh, Lucky Poh Wah Yap, Ho Jin Yong, Eric Wei Choong Lee, Ping Chin TP248.13-248.65 Biotechnology Single nucleotide polymorphisms (SNPs) in the β-like globin gene of the human hosts to the risk of malaria are unclear. Therefore, this study investigates these associations in the Sabah population, with a high incidence of malaria cases. In brief, DNA was extracted from 188 post-diagnostic blood samples infected with Plasmodium parasites and 170 healthy controls without a history of malaria. Genotyping of the β-like globin C-158T, G79A, C16G, and C-551T SNPs was performed using a polymerase chain reaction-restriction fragment length polymorphism approach. Risk association, linkage disequilibrium (LD), and haplotype analyses of these SNPs were assessed. This study found that the variant allele in the C-158T and C16G SNPs were protective against malaria infections by 0.5-fold, while the variant allele in the G79A SNP had a 6-fold increased risk of malaria infection. No SNP combination was in perfect LD, but several haplotypes (CGCC, CGCT, and CGGC) were identified to link with different correlation levels of malaria risk in the population. In conclusion, the C-158T, G79A, and C16G SNPs in the β-like globin gene are associated with the risk of malaria. The haplotypes (CGCC, CGCT, and CGGC) identified in this study could serve as biomarkers to estimate malaria risk in the population. This study provides essential data for the design of malaria control and management strategies. Multidisciplinary Digital Publishing Institute (MDPI) 2022 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/34698/1/FULL%20TEXT.pdf text en https://eprints.ums.edu.my/id/eprint/34698/2/ABSTRACT.pdf Chong, Eric Tzyy Jiann and Goh, Lucky Poh Wah and Yap, Ho Jin and Yong, Eric Wei Choong and Lee, Ping Chin (2022) Risk association, linkage disequilibrium, and haplotype analyses of ß-like globin gene polymorphisms with malaria risk in the Sabah population of Malaysian Borneo. Genes, 13 (1229). pp. 1-11. ISSN 2073-4425 https://www.mdpi.com/2073-4425/13/7/1229/htm https://doi.org/10.3390/genes13071229
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
English
topic TP248.13-248.65 Biotechnology
spellingShingle TP248.13-248.65 Biotechnology
Chong, Eric Tzyy Jiann
Goh, Lucky Poh Wah
Yap, Ho Jin
Yong, Eric Wei Choong
Lee, Ping Chin
Risk association, linkage disequilibrium, and haplotype analyses of ß-like globin gene polymorphisms with malaria risk in the Sabah population of Malaysian Borneo
description Single nucleotide polymorphisms (SNPs) in the β-like globin gene of the human hosts to the risk of malaria are unclear. Therefore, this study investigates these associations in the Sabah population, with a high incidence of malaria cases. In brief, DNA was extracted from 188 post-diagnostic blood samples infected with Plasmodium parasites and 170 healthy controls without a history of malaria. Genotyping of the β-like globin C-158T, G79A, C16G, and C-551T SNPs was performed using a polymerase chain reaction-restriction fragment length polymorphism approach. Risk association, linkage disequilibrium (LD), and haplotype analyses of these SNPs were assessed. This study found that the variant allele in the C-158T and C16G SNPs were protective against malaria infections by 0.5-fold, while the variant allele in the G79A SNP had a 6-fold increased risk of malaria infection. No SNP combination was in perfect LD, but several haplotypes (CGCC, CGCT, and CGGC) were identified to link with different correlation levels of malaria risk in the population. In conclusion, the C-158T, G79A, and C16G SNPs in the β-like globin gene are associated with the risk of malaria. The haplotypes (CGCC, CGCT, and CGGC) identified in this study could serve as biomarkers to estimate malaria risk in the population. This study provides essential data for the design of malaria control and management strategies.
format Article
author Chong, Eric Tzyy Jiann
Goh, Lucky Poh Wah
Yap, Ho Jin
Yong, Eric Wei Choong
Lee, Ping Chin
author_facet Chong, Eric Tzyy Jiann
Goh, Lucky Poh Wah
Yap, Ho Jin
Yong, Eric Wei Choong
Lee, Ping Chin
author_sort Chong, Eric Tzyy Jiann
title Risk association, linkage disequilibrium, and haplotype analyses of ß-like globin gene polymorphisms with malaria risk in the Sabah population of Malaysian Borneo
title_short Risk association, linkage disequilibrium, and haplotype analyses of ß-like globin gene polymorphisms with malaria risk in the Sabah population of Malaysian Borneo
title_full Risk association, linkage disequilibrium, and haplotype analyses of ß-like globin gene polymorphisms with malaria risk in the Sabah population of Malaysian Borneo
title_fullStr Risk association, linkage disequilibrium, and haplotype analyses of ß-like globin gene polymorphisms with malaria risk in the Sabah population of Malaysian Borneo
title_full_unstemmed Risk association, linkage disequilibrium, and haplotype analyses of ß-like globin gene polymorphisms with malaria risk in the Sabah population of Malaysian Borneo
title_sort risk association, linkage disequilibrium, and haplotype analyses of ß-like globin gene polymorphisms with malaria risk in the sabah population of malaysian borneo
publisher Multidisciplinary Digital Publishing Institute (MDPI)
publishDate 2022
url https://eprints.ums.edu.my/id/eprint/34698/1/FULL%20TEXT.pdf
https://eprints.ums.edu.my/id/eprint/34698/2/ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/34698/
https://www.mdpi.com/2073-4425/13/7/1229/htm
https://doi.org/10.3390/genes13071229
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score 13.211869

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