Cover Image

Physiotherapeutic protocol and ZnO nanoparticles: a combined novel treatment program against bacterial pyomyositis

Myositis tropicans or pyomyositis is a muscle inflammation resulting from a bacterial infection of skeletal muscle (commonly caused by Staphylococcus aureus) that usually leads to hematogenous muscle seeding. The present study was designed to estimate the role of ZnO-NPs and a physiotherapeutic prog...

Full description

Saved in:
Bibliographic Details
Main Authors: Hesham El-Shaer, Bassma H. Elwakil, Basant A. Bakr, Ahmed M. Eldrieny, Mostafa El-Khatib, Chong, Khim Phin, Amr A. Abo Gazia
Format: Article
Language:English
English
Published: Multidisciplinary Digital Publishing Institute (MDPI) 2022
Subjects:
QR75-99.5 Bacteria
Online Access:https://eprints.ums.edu.my/id/eprint/34590/1/FULL%20TEXT.pdf
https://eprints.ums.edu.my/id/eprint/34590/2/ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/34590/
https://www.mdpi.com/2079-7737/11/10/1393/htm
https://doi.org/10.3390/biology11101393
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.ums.eprints.34590
record_format eprints
spelling my.ums.eprints.345902022-10-28T07:07:07Z https://eprints.ums.edu.my/id/eprint/34590/ Physiotherapeutic protocol and ZnO nanoparticles: a combined novel treatment program against bacterial pyomyositis Hesham El-Shaer Bassma H. Elwakil Basant A. Bakr Ahmed M. Eldrieny Mostafa El-Khatib Chong, Khim Phin Amr A. Abo Gazia QR75-99.5 Bacteria Myositis tropicans or pyomyositis is a muscle inflammation resulting from a bacterial infection of skeletal muscle (commonly caused by Staphylococcus aureus) that usually leads to hematogenous muscle seeding. The present study was designed to estimate the role of ZnO-NPs and a physiotherapeutic program in the management of induced biceps femoris atrophy in rats through histological, biochemical, and radiological examinations at different time intervals. At the beginning, several bacterial strains were evaluated through a proteolytic enzyme activity assay and the highest activity was recorded with the Staphylococcus aureus strain. ZnO-NPs were synthesized with the arc discharge method with an average size of 19.4 nm. The antibacterial activity of ZnO-NPs was investigated and it was revealed that the prepared ZnO-NPs showed a minimum inhibitory concentration of 8 µg/mL against the tested bacterium. The cytotoxicity of the prepared ZnO-NPs was tested in C2C12 myoblast cells, and it was elaborated that CC50 was 344.16 µg/mL. Biceps femoris pyomyositis was induced with a potent strain (Staphylococcus aureus); then, a physiotherapeutic program combined with the prepared ZnO-NPs treatment protocol was applied and evaluated. The combined program claimed antibacterial properties, preventing muscle atrophy, and resulted in the most comparable value of muscle mass. Multidisciplinary Digital Publishing Institute (MDPI) 2022 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/34590/1/FULL%20TEXT.pdf text en https://eprints.ums.edu.my/id/eprint/34590/2/ABSTRACT.pdf Hesham El-Shaer and Bassma H. Elwakil and Basant A. Bakr and Ahmed M. Eldrieny and Mostafa El-Khatib and Chong, Khim Phin and Amr A. Abo Gazia (2022) Physiotherapeutic protocol and ZnO nanoparticles: a combined novel treatment program against bacterial pyomyositis. Biology, 11 (1393). pp. 1-17. ISSN 2079-7737 https://www.mdpi.com/2079-7737/11/10/1393/htm https://doi.org/10.3390/biology11101393
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
English
topic QR75-99.5 Bacteria
spellingShingle QR75-99.5 Bacteria
Hesham El-Shaer
Bassma H. Elwakil
Basant A. Bakr
Ahmed M. Eldrieny
Mostafa El-Khatib
Chong, Khim Phin
Amr A. Abo Gazia
Physiotherapeutic protocol and ZnO nanoparticles: a combined novel treatment program against bacterial pyomyositis
description Myositis tropicans or pyomyositis is a muscle inflammation resulting from a bacterial infection of skeletal muscle (commonly caused by Staphylococcus aureus) that usually leads to hematogenous muscle seeding. The present study was designed to estimate the role of ZnO-NPs and a physiotherapeutic program in the management of induced biceps femoris atrophy in rats through histological, biochemical, and radiological examinations at different time intervals. At the beginning, several bacterial strains were evaluated through a proteolytic enzyme activity assay and the highest activity was recorded with the Staphylococcus aureus strain. ZnO-NPs were synthesized with the arc discharge method with an average size of 19.4 nm. The antibacterial activity of ZnO-NPs was investigated and it was revealed that the prepared ZnO-NPs showed a minimum inhibitory concentration of 8 µg/mL against the tested bacterium. The cytotoxicity of the prepared ZnO-NPs was tested in C2C12 myoblast cells, and it was elaborated that CC50 was 344.16 µg/mL. Biceps femoris pyomyositis was induced with a potent strain (Staphylococcus aureus); then, a physiotherapeutic program combined with the prepared ZnO-NPs treatment protocol was applied and evaluated. The combined program claimed antibacterial properties, preventing muscle atrophy, and resulted in the most comparable value of muscle mass.
format Article
author Hesham El-Shaer
Bassma H. Elwakil
Basant A. Bakr
Ahmed M. Eldrieny
Mostafa El-Khatib
Chong, Khim Phin
Amr A. Abo Gazia
author_facet Hesham El-Shaer
Bassma H. Elwakil
Basant A. Bakr
Ahmed M. Eldrieny
Mostafa El-Khatib
Chong, Khim Phin
Amr A. Abo Gazia
author_sort Hesham El-Shaer
title Physiotherapeutic protocol and ZnO nanoparticles: a combined novel treatment program against bacterial pyomyositis
title_short Physiotherapeutic protocol and ZnO nanoparticles: a combined novel treatment program against bacterial pyomyositis
title_full Physiotherapeutic protocol and ZnO nanoparticles: a combined novel treatment program against bacterial pyomyositis
title_fullStr Physiotherapeutic protocol and ZnO nanoparticles: a combined novel treatment program against bacterial pyomyositis
title_full_unstemmed Physiotherapeutic protocol and ZnO nanoparticles: a combined novel treatment program against bacterial pyomyositis
title_sort physiotherapeutic protocol and zno nanoparticles: a combined novel treatment program against bacterial pyomyositis
publisher Multidisciplinary Digital Publishing Institute (MDPI)
publishDate 2022
url https://eprints.ums.edu.my/id/eprint/34590/1/FULL%20TEXT.pdf
https://eprints.ums.edu.my/id/eprint/34590/2/ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/34590/
https://www.mdpi.com/2079-7737/11/10/1393/htm
https://doi.org/10.3390/biology11101393
_version_ 1760231316019740672
score 13.211869

Similar Items