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Genetic diversity of circumsporozoite protein in Plasmodium knowlesi isolates from Malaysian Borneo and Peninsular Malaysia

Understanding the genetic diversity of candidate genes for malaria vaccines such as circumsporozoite protein (csp) may enhance the development of vaccines for treating Plasmodium knowlesi. Hence, the aim of this study is to investigate the genetic diversity of non-repeat regions of csp in P. knowles...

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Main Authors: Chong, Eric Tzyy Jiann, Neoh, Joveen Wan Fen, Tiek, Ying Lau, Lim, Yvonne Ai Lian, Hwa, Chia Chai, Kek, Heng Chua, Ping, Chin Lee
Format: Article
Language:English
English
Published: BioMed Central Ltd. 2020
Subjects:
QR180-189.5 Immunology
Online Access:https://eprints.ums.edu.my/id/eprint/34457/1/ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/34457/2/FULLTEXT.pdf.pdf
https://eprints.ums.edu.my/id/eprint/34457/
https://malariajournal.biomedcentral.com/articles/10.1186/s12936-020-03451-x
https://doi.org/10.1186/s12936-020-03451-x
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spelling my.ums.eprints.344572022-10-18T06:24:04Z https://eprints.ums.edu.my/id/eprint/34457/ Genetic diversity of circumsporozoite protein in Plasmodium knowlesi isolates from Malaysian Borneo and Peninsular Malaysia Chong, Eric Tzyy Jiann Neoh, Joveen Wan Fen Tiek, Ying Lau Lim, Yvonne Ai Lian Hwa, Chia Chai Kek, Heng Chua Ping, Chin Lee QR180-189.5 Immunology Understanding the genetic diversity of candidate genes for malaria vaccines such as circumsporozoite protein (csp) may enhance the development of vaccines for treating Plasmodium knowlesi. Hence, the aim of this study is to investigate the genetic diversity of non-repeat regions of csp in P. knowlesi from Malaysian Borneo and Peninsular Malaysia. A total of 46 csp genes were subjected to polymerase chain reaction amplification. The genes were obtained from P. knowlesi isolates collected from different divisions of Sabah, Malaysian Borneo, and Peninsular Malaysia. The targeted gene fragments were cloned into a commercial vector and sequenced, and a phylogenetic tree was constructed while incorporating 168 csp sequences retrieved from the GenBank database. The genetic diversity and natural evolution of the csp sequences were analysed using MEGA6 and DnaSP ver. 5.10.01. A genealogical network of the csp haplotypes was generated using NETWORK ver. 4.6.1.3. The phylogenetic analysis revealed indistinguishable clusters of P. knowlesi isolates across different geographic regions, including Malaysian Borneo and Peninsular Malaysia. Nucleotide analysis showed that the csp nonrepeat regions of zoonotic P. knowlesi isolates obtained in this study underwent purifying selection with population expansion, which was supported by extensive haplotype sharing observed between humans and macaques. Novel variations were observed in the C-terminal non-repeat region of csp. The csp non-repeat regions are relatively conserved and there is no distinct cluster of P. knowlesi isolates from Malaysian Borneo and Peninsular Malaysia. Distinctive variation data obtained in the C-terminal non-repeat region of csp could be beneficial for the design and development of vaccines to treat P. knowlesi. BioMed Central Ltd. 2020 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/34457/1/ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/34457/2/FULLTEXT.pdf.pdf Chong, Eric Tzyy Jiann and Neoh, Joveen Wan Fen and Tiek, Ying Lau and Lim, Yvonne Ai Lian and Hwa, Chia Chai and Kek, Heng Chua and Ping, Chin Lee (2020) Genetic diversity of circumsporozoite protein in Plasmodium knowlesi isolates from Malaysian Borneo and Peninsular Malaysia. Malaria Journal, 19. pp. 1-7. ISSN 1475-2875 https://malariajournal.biomedcentral.com/articles/10.1186/s12936-020-03451-x https://doi.org/10.1186/s12936-020-03451-x
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
English
topic QR180-189.5 Immunology
spellingShingle QR180-189.5 Immunology
Chong, Eric Tzyy Jiann
Neoh, Joveen Wan Fen
Tiek, Ying Lau
Lim, Yvonne Ai Lian
Hwa, Chia Chai
Kek, Heng Chua
Ping, Chin Lee
Genetic diversity of circumsporozoite protein in Plasmodium knowlesi isolates from Malaysian Borneo and Peninsular Malaysia
description Understanding the genetic diversity of candidate genes for malaria vaccines such as circumsporozoite protein (csp) may enhance the development of vaccines for treating Plasmodium knowlesi. Hence, the aim of this study is to investigate the genetic diversity of non-repeat regions of csp in P. knowlesi from Malaysian Borneo and Peninsular Malaysia. A total of 46 csp genes were subjected to polymerase chain reaction amplification. The genes were obtained from P. knowlesi isolates collected from different divisions of Sabah, Malaysian Borneo, and Peninsular Malaysia. The targeted gene fragments were cloned into a commercial vector and sequenced, and a phylogenetic tree was constructed while incorporating 168 csp sequences retrieved from the GenBank database. The genetic diversity and natural evolution of the csp sequences were analysed using MEGA6 and DnaSP ver. 5.10.01. A genealogical network of the csp haplotypes was generated using NETWORK ver. 4.6.1.3. The phylogenetic analysis revealed indistinguishable clusters of P. knowlesi isolates across different geographic regions, including Malaysian Borneo and Peninsular Malaysia. Nucleotide analysis showed that the csp nonrepeat regions of zoonotic P. knowlesi isolates obtained in this study underwent purifying selection with population expansion, which was supported by extensive haplotype sharing observed between humans and macaques. Novel variations were observed in the C-terminal non-repeat region of csp. The csp non-repeat regions are relatively conserved and there is no distinct cluster of P. knowlesi isolates from Malaysian Borneo and Peninsular Malaysia. Distinctive variation data obtained in the C-terminal non-repeat region of csp could be beneficial for the design and development of vaccines to treat P. knowlesi.
format Article
author Chong, Eric Tzyy Jiann
Neoh, Joveen Wan Fen
Tiek, Ying Lau
Lim, Yvonne Ai Lian
Hwa, Chia Chai
Kek, Heng Chua
Ping, Chin Lee
author_facet Chong, Eric Tzyy Jiann
Neoh, Joveen Wan Fen
Tiek, Ying Lau
Lim, Yvonne Ai Lian
Hwa, Chia Chai
Kek, Heng Chua
Ping, Chin Lee
author_sort Chong, Eric Tzyy Jiann
title Genetic diversity of circumsporozoite protein in Plasmodium knowlesi isolates from Malaysian Borneo and Peninsular Malaysia
title_short Genetic diversity of circumsporozoite protein in Plasmodium knowlesi isolates from Malaysian Borneo and Peninsular Malaysia
title_full Genetic diversity of circumsporozoite protein in Plasmodium knowlesi isolates from Malaysian Borneo and Peninsular Malaysia
title_fullStr Genetic diversity of circumsporozoite protein in Plasmodium knowlesi isolates from Malaysian Borneo and Peninsular Malaysia
title_full_unstemmed Genetic diversity of circumsporozoite protein in Plasmodium knowlesi isolates from Malaysian Borneo and Peninsular Malaysia
title_sort genetic diversity of circumsporozoite protein in plasmodium knowlesi isolates from malaysian borneo and peninsular malaysia
publisher BioMed Central Ltd.
publishDate 2020
url https://eprints.ums.edu.my/id/eprint/34457/1/ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/34457/2/FULLTEXT.pdf.pdf
https://eprints.ums.edu.my/id/eprint/34457/
https://malariajournal.biomedcentral.com/articles/10.1186/s12936-020-03451-x
https://doi.org/10.1186/s12936-020-03451-x
_version_ 1760231297987379200
score 13.211869

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