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Chemical Composition and the Anticancer, Antimicrobial, and Antioxidant Properties of Acacia Honey from the Hail Region: The in vitro and in silico Investigation

In consideration of the emergence of novel drug-resistant microbial strains and the increase in the incidences of various cancers throughout the world, honey could be utilized as a great alternative source of potent bioactive compounds. In this context, this study pioneers in reporting the phytochem...

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Main Authors: Walid Sabri Hamadou, Nouha Bouali, Riadh Badraoui, Ramzi Hadj Lajimi, Assia Hamdi, Mousa Alreshidi, Mitesh Patel, Mohd Adnan, Arif Jamal Siddiqui, Emira Noumi, Pasupuleti Visweswara Rao, Mejdi Snoussi
Format: Article
Language:English
English
Published: Hindawi Publishing Corporation 2022
Subjects:
RC254-282 Neoplasms. Tumors. Oncology Including cancer and carcinogens
Online Access:https://eprints.ums.edu.my/id/eprint/34289/3/FULL%20TEXT.pdf
https://eprints.ums.edu.my/id/eprint/34289/5/ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/34289/
https://downloads.hindawi.com/journals/ecam/2022/1518511.pdf
http://dx.doi.org/10.1155/2022/1518511
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spelling my.ums.eprints.342892022-09-27T03:43:35Z https://eprints.ums.edu.my/id/eprint/34289/ Chemical Composition and the Anticancer, Antimicrobial, and Antioxidant Properties of Acacia Honey from the Hail Region: The in vitro and in silico Investigation Walid Sabri Hamadou Nouha Bouali Riadh Badraoui Ramzi Hadj Lajimi Assia Hamdi Mousa Alreshidi Mitesh Patel Mohd Adnan Arif Jamal Siddiqui Emira Noumi Pasupuleti Visweswara Rao Mejdi Snoussi RC254-282 Neoplasms. Tumors. Oncology Including cancer and carcinogens In consideration of the emergence of novel drug-resistant microbial strains and the increase in the incidences of various cancers throughout the world, honey could be utilized as a great alternative source of potent bioactive compounds. In this context, this study pioneers in reporting the phytochemical profiling and the antimicrobial, antioxidant, and anticancer properties of Acacia honey (AH) from the Hail region of Saudi Arabia, assessed using in vitro and molecular docking approaches. The phytochemical profiling based on high-resolution liquid chromatography-mass spectrometry (HR-LCMS) revealed eight compounds and three small peptide-like proteins as the constituents. The honey samples exhibited promising antioxidant activities (DPPH-IC50 = 0.670 mg/mL; ABTS-IC50 = 1.056 mg/mL; β-carotene-IC50 > 5 mg/mL). In the well-diffusion assay, a high mean growth inhibition zone (mGIZ) was observed against Staphylococcus aureus (48.33 ± 1.53 mm), Escherichia coli ATCC 10536 (38.33 ± 1.53 mm), and Staphylococcus epidermidis ATCC 12228 (39.33 ± 1.15 mm). The microdilution assay revealed that low concentrations of AH could inhibit the growth of almost all the evaluated bacterial and fungal strains, with the minimal bactericidal concentration values (MBCs) ranging from 75 mg/mL to 300 mg/mL. On the contrary, high AH concentrations were required to kill the tested microorganisms, with the minimal bactericidal concentration values (MBCs) ranging from approximately 300 mg/mL to over 600 mg/mL and the minimal fungicidal concentration values (MFCs) of approximately 600 mg/mL. The AH exhibited effective anticancer activity in a dose-dependent manner against breast (MCF-7), colon (HCT-116), and lung (A549) cancer cell lines, with the corresponding IC50 values of 5.053 μg/mL, 5.382 μg/mL, and 6.728 μg/mL, respectively. The in silico investigation revealed that the observed antimicrobial, antioxidant, and anticancer activities of the constituent compounds of AH are thermodynamically feasible, particularly those of the tripeptides (Asp-Trp-His and Trp-Arg-Ala) and aminocyclitol glycoside. The overall results highlighted the potential of AH as a source of bioactive compounds with significant antimicrobial, antioxidant, and anticancer activities, which could imply further pharmacological applications of AH. Hindawi Publishing Corporation 2022 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/34289/3/FULL%20TEXT.pdf text en https://eprints.ums.edu.my/id/eprint/34289/5/ABSTRACT.pdf Walid Sabri Hamadou and Nouha Bouali and Riadh Badraoui and Ramzi Hadj Lajimi and Assia Hamdi and Mousa Alreshidi and Mitesh Patel and Mohd Adnan and Arif Jamal Siddiqui and Emira Noumi and Pasupuleti Visweswara Rao and Mejdi Snoussi (2022) Chemical Composition and the Anticancer, Antimicrobial, and Antioxidant Properties of Acacia Honey from the Hail Region: The in vitro and in silico Investigation. Evidence-Based Complementary and Alternative Medicine, 2022. pp. 1-16. ISSN 1741-427X (P-ISSN) , 1741-4288 (E-ISSN) https://downloads.hindawi.com/journals/ecam/2022/1518511.pdf http://dx.doi.org/10.1155/2022/1518511
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
English
topic RC254-282 Neoplasms. Tumors. Oncology Including cancer and carcinogens
spellingShingle RC254-282 Neoplasms. Tumors. Oncology Including cancer and carcinogens
Walid Sabri Hamadou
Nouha Bouali
Riadh Badraoui
Ramzi Hadj Lajimi
Assia Hamdi
Mousa Alreshidi
Mitesh Patel
Mohd Adnan
Arif Jamal Siddiqui
Emira Noumi
Pasupuleti Visweswara Rao
Mejdi Snoussi
Chemical Composition and the Anticancer, Antimicrobial, and Antioxidant Properties of Acacia Honey from the Hail Region: The in vitro and in silico Investigation
description In consideration of the emergence of novel drug-resistant microbial strains and the increase in the incidences of various cancers throughout the world, honey could be utilized as a great alternative source of potent bioactive compounds. In this context, this study pioneers in reporting the phytochemical profiling and the antimicrobial, antioxidant, and anticancer properties of Acacia honey (AH) from the Hail region of Saudi Arabia, assessed using in vitro and molecular docking approaches. The phytochemical profiling based on high-resolution liquid chromatography-mass spectrometry (HR-LCMS) revealed eight compounds and three small peptide-like proteins as the constituents. The honey samples exhibited promising antioxidant activities (DPPH-IC50 = 0.670 mg/mL; ABTS-IC50 = 1.056 mg/mL; β-carotene-IC50 > 5 mg/mL). In the well-diffusion assay, a high mean growth inhibition zone (mGIZ) was observed against Staphylococcus aureus (48.33 ± 1.53 mm), Escherichia coli ATCC 10536 (38.33 ± 1.53 mm), and Staphylococcus epidermidis ATCC 12228 (39.33 ± 1.15 mm). The microdilution assay revealed that low concentrations of AH could inhibit the growth of almost all the evaluated bacterial and fungal strains, with the minimal bactericidal concentration values (MBCs) ranging from 75 mg/mL to 300 mg/mL. On the contrary, high AH concentrations were required to kill the tested microorganisms, with the minimal bactericidal concentration values (MBCs) ranging from approximately 300 mg/mL to over 600 mg/mL and the minimal fungicidal concentration values (MFCs) of approximately 600 mg/mL. The AH exhibited effective anticancer activity in a dose-dependent manner against breast (MCF-7), colon (HCT-116), and lung (A549) cancer cell lines, with the corresponding IC50 values of 5.053 μg/mL, 5.382 μg/mL, and 6.728 μg/mL, respectively. The in silico investigation revealed that the observed antimicrobial, antioxidant, and anticancer activities of the constituent compounds of AH are thermodynamically feasible, particularly those of the tripeptides (Asp-Trp-His and Trp-Arg-Ala) and aminocyclitol glycoside. The overall results highlighted the potential of AH as a source of bioactive compounds with significant antimicrobial, antioxidant, and anticancer activities, which could imply further pharmacological applications of AH.
format Article
author Walid Sabri Hamadou
Nouha Bouali
Riadh Badraoui
Ramzi Hadj Lajimi
Assia Hamdi
Mousa Alreshidi
Mitesh Patel
Mohd Adnan
Arif Jamal Siddiqui
Emira Noumi
Pasupuleti Visweswara Rao
Mejdi Snoussi
author_facet Walid Sabri Hamadou
Nouha Bouali
Riadh Badraoui
Ramzi Hadj Lajimi
Assia Hamdi
Mousa Alreshidi
Mitesh Patel
Mohd Adnan
Arif Jamal Siddiqui
Emira Noumi
Pasupuleti Visweswara Rao
Mejdi Snoussi
author_sort Walid Sabri Hamadou
title Chemical Composition and the Anticancer, Antimicrobial, and Antioxidant Properties of Acacia Honey from the Hail Region: The in vitro and in silico Investigation
title_short Chemical Composition and the Anticancer, Antimicrobial, and Antioxidant Properties of Acacia Honey from the Hail Region: The in vitro and in silico Investigation
title_full Chemical Composition and the Anticancer, Antimicrobial, and Antioxidant Properties of Acacia Honey from the Hail Region: The in vitro and in silico Investigation
title_fullStr Chemical Composition and the Anticancer, Antimicrobial, and Antioxidant Properties of Acacia Honey from the Hail Region: The in vitro and in silico Investigation
title_full_unstemmed Chemical Composition and the Anticancer, Antimicrobial, and Antioxidant Properties of Acacia Honey from the Hail Region: The in vitro and in silico Investigation
title_sort chemical composition and the anticancer, antimicrobial, and antioxidant properties of acacia honey from the hail region: the in vitro and in silico investigation
publisher Hindawi Publishing Corporation
publishDate 2022
url https://eprints.ums.edu.my/id/eprint/34289/3/FULL%20TEXT.pdf
https://eprints.ums.edu.my/id/eprint/34289/5/ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/34289/
https://downloads.hindawi.com/journals/ecam/2022/1518511.pdf
http://dx.doi.org/10.1155/2022/1518511
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score 13.211869

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