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CD4 cell activation with the CD8 marker and metallothionein expression in the gills of cadmium-exposed mosquito fish (Gambusia affinis Baird and Girard 1853) juveniles

This experiment aimed to determine how Cd exposure impacts CD4 cell activation, macrophage cells, pinocytosis activity, metallothionine expression, and Cd levels in juvenile Gambusia affinis gills. Four treatment groups were used, with one control and treatments A, B, C, and D at dosages of 0.03 mg/...

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Main Authors: Moh Awaludin Adam, Agoes Soegianto, Carolyn Melissa Payus, Ach Khumaidi, Ramli Ramli, Ernawati Ernawati, Irawati Mei Widiastuti, Era Insivitawati, Yenny Risjani
Format: Article
Language:English
English
Published: Elsevier B.V. 2022
Subjects:
QL614-639.8 Fishes
Online Access:https://eprints.ums.edu.my/id/eprint/33955/1/CD4%20cell%20activation%20with%20the%20CD8%20marker%20and%20metallothionein%20expression%20in%20the%20gills%20of%20cadmium-exposed%20mosquito%20fish%20%28Gambusia%20affinis%20Baird%20and%20Girard%201853%29%20juveniles.pdf
https://eprints.ums.edu.my/id/eprint/33955/2/CD4%20cell%20activation%20with%20the%20CD8%20marker%20and%20metallothionein%20expression%20in%20the%20gills%20of%20cadmium-exposed%20mosquito%20fish%20%28Gambusia%20affinis%20Baird%20and%20Girard%201853%29%20juveniles%20_ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/33955/
https://www.sciencedirect.com/science/article/pii/S2405665022000178
https://doi.org/10.1016/j.emcon.2022.05.002
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spelling my.ums.eprints.339552022-08-26T00:25:20Z https://eprints.ums.edu.my/id/eprint/33955/ CD4 cell activation with the CD8 marker and metallothionein expression in the gills of cadmium-exposed mosquito fish (Gambusia affinis Baird and Girard 1853) juveniles Moh Awaludin Adam Agoes Soegianto Carolyn Melissa Payus Ach Khumaidi Ramli Ramli Ernawati Ernawati Irawati Mei Widiastuti Era Insivitawati Yenny Risjani QL614-639.8 Fishes This experiment aimed to determine how Cd exposure impacts CD4 cell activation, macrophage cells, pinocytosis activity, metallothionine expression, and Cd levels in juvenile Gambusia affinis gills. Four treatment groups were used, with one control and treatments A, B, C, and D at dosages of 0.03 mg/L, 0.023 mg/L, 0.015 mg/L, and 0.008 mg/L, respectively. The results showed that the number of CD4 with CD8 cell markers differed significantly from each treatment compared to the control (0.33%). The value in A, namely 0.54%, was the highest, followed by B, C, and D of 0.46%, 0.44%, and 0.42%, respectively. The number of macrophages increased as the Cd level of the medium increased, as did the activity of pinocytosis. Furthermore, the immunofluorescence test on the gills with the Anti-MT Mouse and goat IgG fluoresce in Rhodamine on the gills showed that luminescence increased with increasing Cd levels in the gills. Similarly, the MT intensity increased at Cd-exposed gills as compared to the control. As the Cd level in the gills increased, the MT levels ascended significantly. Elsevier B.V. 2022 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/33955/1/CD4%20cell%20activation%20with%20the%20CD8%20marker%20and%20metallothionein%20expression%20in%20the%20gills%20of%20cadmium-exposed%20mosquito%20fish%20%28Gambusia%20affinis%20Baird%20and%20Girard%201853%29%20juveniles.pdf text en https://eprints.ums.edu.my/id/eprint/33955/2/CD4%20cell%20activation%20with%20the%20CD8%20marker%20and%20metallothionein%20expression%20in%20the%20gills%20of%20cadmium-exposed%20mosquito%20fish%20%28Gambusia%20affinis%20Baird%20and%20Girard%201853%29%20juveniles%20_ABSTRACT.pdf Moh Awaludin Adam and Agoes Soegianto and Carolyn Melissa Payus and Ach Khumaidi and Ramli Ramli and Ernawati Ernawati and Irawati Mei Widiastuti and Era Insivitawati and Yenny Risjani (2022) CD4 cell activation with the CD8 marker and metallothionein expression in the gills of cadmium-exposed mosquito fish (Gambusia affinis Baird and Girard 1853) juveniles. Emerging Contaminants, 8. pp. 280-287. ISSN 2405-6650 https://www.sciencedirect.com/science/article/pii/S2405665022000178 https://doi.org/10.1016/j.emcon.2022.05.002
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
English
topic QL614-639.8 Fishes
spellingShingle QL614-639.8 Fishes
Moh Awaludin Adam
Agoes Soegianto
Carolyn Melissa Payus
Ach Khumaidi
Ramli Ramli
Ernawati Ernawati
Irawati Mei Widiastuti
Era Insivitawati
Yenny Risjani
CD4 cell activation with the CD8 marker and metallothionein expression in the gills of cadmium-exposed mosquito fish (Gambusia affinis Baird and Girard 1853) juveniles
description This experiment aimed to determine how Cd exposure impacts CD4 cell activation, macrophage cells, pinocytosis activity, metallothionine expression, and Cd levels in juvenile Gambusia affinis gills. Four treatment groups were used, with one control and treatments A, B, C, and D at dosages of 0.03 mg/L, 0.023 mg/L, 0.015 mg/L, and 0.008 mg/L, respectively. The results showed that the number of CD4 with CD8 cell markers differed significantly from each treatment compared to the control (0.33%). The value in A, namely 0.54%, was the highest, followed by B, C, and D of 0.46%, 0.44%, and 0.42%, respectively. The number of macrophages increased as the Cd level of the medium increased, as did the activity of pinocytosis. Furthermore, the immunofluorescence test on the gills with the Anti-MT Mouse and goat IgG fluoresce in Rhodamine on the gills showed that luminescence increased with increasing Cd levels in the gills. Similarly, the MT intensity increased at Cd-exposed gills as compared to the control. As the Cd level in the gills increased, the MT levels ascended significantly.
format Article
author Moh Awaludin Adam
Agoes Soegianto
Carolyn Melissa Payus
Ach Khumaidi
Ramli Ramli
Ernawati Ernawati
Irawati Mei Widiastuti
Era Insivitawati
Yenny Risjani
author_facet Moh Awaludin Adam
Agoes Soegianto
Carolyn Melissa Payus
Ach Khumaidi
Ramli Ramli
Ernawati Ernawati
Irawati Mei Widiastuti
Era Insivitawati
Yenny Risjani
author_sort Moh Awaludin Adam
title CD4 cell activation with the CD8 marker and metallothionein expression in the gills of cadmium-exposed mosquito fish (Gambusia affinis Baird and Girard 1853) juveniles
title_short CD4 cell activation with the CD8 marker and metallothionein expression in the gills of cadmium-exposed mosquito fish (Gambusia affinis Baird and Girard 1853) juveniles
title_full CD4 cell activation with the CD8 marker and metallothionein expression in the gills of cadmium-exposed mosquito fish (Gambusia affinis Baird and Girard 1853) juveniles
title_fullStr CD4 cell activation with the CD8 marker and metallothionein expression in the gills of cadmium-exposed mosquito fish (Gambusia affinis Baird and Girard 1853) juveniles
title_full_unstemmed CD4 cell activation with the CD8 marker and metallothionein expression in the gills of cadmium-exposed mosquito fish (Gambusia affinis Baird and Girard 1853) juveniles
title_sort cd4 cell activation with the cd8 marker and metallothionein expression in the gills of cadmium-exposed mosquito fish (gambusia affinis baird and girard 1853) juveniles
publisher Elsevier B.V.
publishDate 2022
url https://eprints.ums.edu.my/id/eprint/33955/1/CD4%20cell%20activation%20with%20the%20CD8%20marker%20and%20metallothionein%20expression%20in%20the%20gills%20of%20cadmium-exposed%20mosquito%20fish%20%28Gambusia%20affinis%20Baird%20and%20Girard%201853%29%20juveniles.pdf
https://eprints.ums.edu.my/id/eprint/33955/2/CD4%20cell%20activation%20with%20the%20CD8%20marker%20and%20metallothionein%20expression%20in%20the%20gills%20of%20cadmium-exposed%20mosquito%20fish%20%28Gambusia%20affinis%20Baird%20and%20Girard%201853%29%20juveniles%20_ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/33955/
https://www.sciencedirect.com/science/article/pii/S2405665022000178
https://doi.org/10.1016/j.emcon.2022.05.002
_version_ 1760231231408046080
score 13.211869

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