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Profiling of MicroRNA expression in obese and diabetic-induced mice for biomarker discovery

MicroRNAs (miRNAs) are short (-22 nucleotides) regulatory RNAs involved in many fundamental biological processes. They are involved in post-transcriptional regulation of gene expression. Dysregulated expression of microRNAs has been associated with a variety of diseases, including obesity and diabet...

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Main Authors: Janan N. Hadi, Mohammad Iqbal, Vijay Kumar Subbiah
Format: Article
Language:English
English
Published: UniSE Press 2017
Subjects:
QP501-801 Animal biochemistry
RC648-665 Diseases of the endocrine glands. Clinical endocrinology
Online Access:https://eprints.ums.edu.my/id/eprint/33506/1/Profiling%20of%20MicroRNA%20expression%20in%20obese%20and%20diabetic-induced%20mice%20for%20biomarker%20discovery.ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/33506/2/profiling%20of%20microrna%20expression%20in%20obese%20and%20diabetic-induced%20for%20biomarker%20discovery.pdf
https://eprints.ums.edu.my/id/eprint/33506/
http://tost.unise.org/pdfs/vol4/no3-3/4x3-3x391x395.pdf
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spelling my.ums.eprints.335062022-07-23T03:07:01Z https://eprints.ums.edu.my/id/eprint/33506/ Profiling of MicroRNA expression in obese and diabetic-induced mice for biomarker discovery Janan N. Hadi Mohammad Iqbal Vijay Kumar Subbiah QP501-801 Animal biochemistry RC648-665 Diseases of the endocrine glands. Clinical endocrinology MicroRNAs (miRNAs) are short (-22 nucleotides) regulatory RNAs involved in many fundamental biological processes. They are involved in post-transcriptional regulation of gene expression. Dysregulated expression of microRNAs has been associated with a variety of diseases, including obesity and diabetes. Obesity is a potential risk factor contributing to the development of type 2 diabetes. Meanwhile, diabetes is one of the most prevalent chronic diseases, affecting 6.4% of the world’s adult population. The aim of this study is to identify microRNAs that are differentially expressed in obese, diabetic and control C57BL/6 mice by using small RNA sequencing. Total RNAs were extracted from the serum of the target groups of animals. Next, the small RNAs were sequenced using the TruSeq small RNA Library Prep Kit in a MiSeq Illumina sequencer. A total of 52 up-regulated and 54 down-regulated miRNAs were identified based on the comparison of the log2 fold change of obese and diabetic (with normal mice as control; FC ≥ 2). The obese groups showed 22 up-regulated and 25 down-regulated microRNAs. Meanwhile, in the diabetic group, 32 microRNAs were up-regulated and 29 were down-regulated. This finding will help better understand the mechanism of metabolic disorders and may influence future approaches for the diagnosis and treatment of obesity and diabetes. UniSE Press 2017-11-28 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/33506/1/Profiling%20of%20MicroRNA%20expression%20in%20obese%20and%20diabetic-induced%20mice%20for%20biomarker%20discovery.ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/33506/2/profiling%20of%20microrna%20expression%20in%20obese%20and%20diabetic-induced%20for%20biomarker%20discovery.pdf Janan N. Hadi and Mohammad Iqbal and Vijay Kumar Subbiah (2017) Profiling of MicroRNA expression in obese and diabetic-induced mice for biomarker discovery. Transactions on Science and Technology, 4 (3-3). pp. 391-395. ISSN 2289-8786 http://tost.unise.org/pdfs/vol4/no3-3/4x3-3x391x395.pdf
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
English
topic QP501-801 Animal biochemistry
RC648-665 Diseases of the endocrine glands. Clinical endocrinology
spellingShingle QP501-801 Animal biochemistry
RC648-665 Diseases of the endocrine glands. Clinical endocrinology
Janan N. Hadi
Mohammad Iqbal
Vijay Kumar Subbiah
Profiling of MicroRNA expression in obese and diabetic-induced mice for biomarker discovery
description MicroRNAs (miRNAs) are short (-22 nucleotides) regulatory RNAs involved in many fundamental biological processes. They are involved in post-transcriptional regulation of gene expression. Dysregulated expression of microRNAs has been associated with a variety of diseases, including obesity and diabetes. Obesity is a potential risk factor contributing to the development of type 2 diabetes. Meanwhile, diabetes is one of the most prevalent chronic diseases, affecting 6.4% of the world’s adult population. The aim of this study is to identify microRNAs that are differentially expressed in obese, diabetic and control C57BL/6 mice by using small RNA sequencing. Total RNAs were extracted from the serum of the target groups of animals. Next, the small RNAs were sequenced using the TruSeq small RNA Library Prep Kit in a MiSeq Illumina sequencer. A total of 52 up-regulated and 54 down-regulated miRNAs were identified based on the comparison of the log2 fold change of obese and diabetic (with normal mice as control; FC ≥ 2). The obese groups showed 22 up-regulated and 25 down-regulated microRNAs. Meanwhile, in the diabetic group, 32 microRNAs were up-regulated and 29 were down-regulated. This finding will help better understand the mechanism of metabolic disorders and may influence future approaches for the diagnosis and treatment of obesity and diabetes.
format Article
author Janan N. Hadi
Mohammad Iqbal
Vijay Kumar Subbiah
author_facet Janan N. Hadi
Mohammad Iqbal
Vijay Kumar Subbiah
author_sort Janan N. Hadi
title Profiling of MicroRNA expression in obese and diabetic-induced mice for biomarker discovery
title_short Profiling of MicroRNA expression in obese and diabetic-induced mice for biomarker discovery
title_full Profiling of MicroRNA expression in obese and diabetic-induced mice for biomarker discovery
title_fullStr Profiling of MicroRNA expression in obese and diabetic-induced mice for biomarker discovery
title_full_unstemmed Profiling of MicroRNA expression in obese and diabetic-induced mice for biomarker discovery
title_sort profiling of microrna expression in obese and diabetic-induced mice for biomarker discovery
publisher UniSE Press
publishDate 2017
url https://eprints.ums.edu.my/id/eprint/33506/1/Profiling%20of%20MicroRNA%20expression%20in%20obese%20and%20diabetic-induced%20mice%20for%20biomarker%20discovery.ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/33506/2/profiling%20of%20microrna%20expression%20in%20obese%20and%20diabetic-induced%20for%20biomarker%20discovery.pdf
https://eprints.ums.edu.my/id/eprint/33506/
http://tost.unise.org/pdfs/vol4/no3-3/4x3-3x391x395.pdf
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score 13.211869

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