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Ethyl acetate partition obtained from the methanol extract of Muntingia calabura leaf exerts effective in vitro antiproliferative activity against the HT-29 colon cancer

Methanol extract of Muntingia calabura L. leaf (MEMCL) has been shown to exert the antiproliferative activity against the HT-29 (human colon adenocarcinoma) cell line. To further investigate on the medicinal potential of this plant, MEMCL was sequentially partitioned to obtain the petroleum ether, e...

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Main Authors: Zainul Amirudin Zakaria, Nur Liana Md Nasir, Noorsyaza Eddrina Kamsani, Huzwah Khaza’ai, Lilis Sulistyorini, Roro Azizah
Format: Article
Language:English
English
Published: Blacpma 2022
Subjects:
RC254-282 Neoplasms. Tumors. Oncology Including cancer and carcinogens
RV1-431 Botanic, Thomsonian, and eclectic medicine
Online Access:https://eprints.ums.edu.my/id/eprint/33276/1/Ethyl%20acetate%20partition%20obtained%20from%20the%20methanol%20extract%20of%20Muntingia%20calabura%20leaf%20exerts%20effective%20in%20vitro%20antiproliferative%20activity%20against%20the%20HT-29%20colon%20cancer%20.pdf
https://eprints.ums.edu.my/id/eprint/33276/2/Ethyl%20acetate%20partition%20obtained%20from%20the%20methanol%20extract%20of%20Muntingia%20calabura%20leaf%20exerts%20effective%20in%20vitro%20antiproliferative%20activity%20against%20the%20HT-29%20colon%20cancer%201.pdf
https://eprints.ums.edu.my/id/eprint/33276/
https://www.blacpma.ms-editions.cl/index.php/blacpma/article/view/310/318
https://doi.org/10.37360/blacpma.22.21.5.41
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spelling my.ums.eprints.332762022-07-16T10:39:39Z https://eprints.ums.edu.my/id/eprint/33276/ Ethyl acetate partition obtained from the methanol extract of Muntingia calabura leaf exerts effective in vitro antiproliferative activity against the HT-29 colon cancer Zainul Amirudin Zakaria Nur Liana Md Nasir Noorsyaza Eddrina Kamsani Huzwah Khaza’ai Lilis Sulistyorini Roro Azizah RC254-282 Neoplasms. Tumors. Oncology Including cancer and carcinogens RV1-431 Botanic, Thomsonian, and eclectic medicine Methanol extract of Muntingia calabura L. leaf (MEMCL) has been shown to exert the antiproliferative activity against the HT-29 (human colon adenocarcinoma) cell line. To further investigate on the medicinal potential of this plant, MEMCL was sequentially partitioned to obtain the petroleum ether, ethyl acetate and aqueous partitions, which was then tested against the HT-29 cell line and also subjected to the in vitro anti-inflammatory study. The most effective partition was also subjected to the phytoconstituents analysis using the UHPLC-ESI-MS. Findings showed that the ethyl acetate partition (EAP) exerts the most effective antiproliferative activity (IC50 = 58.0 ± 12.9 µg/mL) without affecting the 3T3 normal fibroblast cells, exhibits the highest anti-inflammatory effect when assessed using the lipoxygenase (> 95%) and xanthine oxidase (> 70%) assays, and contained various types of polyphenolics. In conclusion, M. calabura exerts apoptotic-mediated antiproliferative activity, partly via the anti-inflammatory action and synergistic action between the polyphenolics. Blacpma 2022 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/33276/1/Ethyl%20acetate%20partition%20obtained%20from%20the%20methanol%20extract%20of%20Muntingia%20calabura%20leaf%20exerts%20effective%20in%20vitro%20antiproliferative%20activity%20against%20the%20HT-29%20colon%20cancer%20.pdf text en https://eprints.ums.edu.my/id/eprint/33276/2/Ethyl%20acetate%20partition%20obtained%20from%20the%20methanol%20extract%20of%20Muntingia%20calabura%20leaf%20exerts%20effective%20in%20vitro%20antiproliferative%20activity%20against%20the%20HT-29%20colon%20cancer%201.pdf Zainul Amirudin Zakaria and Nur Liana Md Nasir and Noorsyaza Eddrina Kamsani and Huzwah Khaza’ai and Lilis Sulistyorini and Roro Azizah (2022) Ethyl acetate partition obtained from the methanol extract of Muntingia calabura leaf exerts effective in vitro antiproliferative activity against the HT-29 colon cancer. Boletin Latinoamericano Y Del Caribe De Plantas Medicinales Y Aromáticas, 21. pp. 654-670. ISSN 0717-7917 https://www.blacpma.ms-editions.cl/index.php/blacpma/article/view/310/318 https://doi.org/10.37360/blacpma.22.21.5.41
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
English
topic RC254-282 Neoplasms. Tumors. Oncology Including cancer and carcinogens
RV1-431 Botanic, Thomsonian, and eclectic medicine
spellingShingle RC254-282 Neoplasms. Tumors. Oncology Including cancer and carcinogens
RV1-431 Botanic, Thomsonian, and eclectic medicine
Zainul Amirudin Zakaria
Nur Liana Md Nasir
Noorsyaza Eddrina Kamsani
Huzwah Khaza’ai
Lilis Sulistyorini
Roro Azizah
Ethyl acetate partition obtained from the methanol extract of Muntingia calabura leaf exerts effective in vitro antiproliferative activity against the HT-29 colon cancer
description Methanol extract of Muntingia calabura L. leaf (MEMCL) has been shown to exert the antiproliferative activity against the HT-29 (human colon adenocarcinoma) cell line. To further investigate on the medicinal potential of this plant, MEMCL was sequentially partitioned to obtain the petroleum ether, ethyl acetate and aqueous partitions, which was then tested against the HT-29 cell line and also subjected to the in vitro anti-inflammatory study. The most effective partition was also subjected to the phytoconstituents analysis using the UHPLC-ESI-MS. Findings showed that the ethyl acetate partition (EAP) exerts the most effective antiproliferative activity (IC50 = 58.0 ± 12.9 µg/mL) without affecting the 3T3 normal fibroblast cells, exhibits the highest anti-inflammatory effect when assessed using the lipoxygenase (> 95%) and xanthine oxidase (> 70%) assays, and contained various types of polyphenolics. In conclusion, M. calabura exerts apoptotic-mediated antiproliferative activity, partly via the anti-inflammatory action and synergistic action between the polyphenolics.
format Article
author Zainul Amirudin Zakaria
Nur Liana Md Nasir
Noorsyaza Eddrina Kamsani
Huzwah Khaza’ai
Lilis Sulistyorini
Roro Azizah
author_facet Zainul Amirudin Zakaria
Nur Liana Md Nasir
Noorsyaza Eddrina Kamsani
Huzwah Khaza’ai
Lilis Sulistyorini
Roro Azizah
author_sort Zainul Amirudin Zakaria
title Ethyl acetate partition obtained from the methanol extract of Muntingia calabura leaf exerts effective in vitro antiproliferative activity against the HT-29 colon cancer
title_short Ethyl acetate partition obtained from the methanol extract of Muntingia calabura leaf exerts effective in vitro antiproliferative activity against the HT-29 colon cancer
title_full Ethyl acetate partition obtained from the methanol extract of Muntingia calabura leaf exerts effective in vitro antiproliferative activity against the HT-29 colon cancer
title_fullStr Ethyl acetate partition obtained from the methanol extract of Muntingia calabura leaf exerts effective in vitro antiproliferative activity against the HT-29 colon cancer
title_full_unstemmed Ethyl acetate partition obtained from the methanol extract of Muntingia calabura leaf exerts effective in vitro antiproliferative activity against the HT-29 colon cancer
title_sort ethyl acetate partition obtained from the methanol extract of muntingia calabura leaf exerts effective in vitro antiproliferative activity against the ht-29 colon cancer
publisher Blacpma
publishDate 2022
url https://eprints.ums.edu.my/id/eprint/33276/1/Ethyl%20acetate%20partition%20obtained%20from%20the%20methanol%20extract%20of%20Muntingia%20calabura%20leaf%20exerts%20effective%20in%20vitro%20antiproliferative%20activity%20against%20the%20HT-29%20colon%20cancer%20.pdf
https://eprints.ums.edu.my/id/eprint/33276/2/Ethyl%20acetate%20partition%20obtained%20from%20the%20methanol%20extract%20of%20Muntingia%20calabura%20leaf%20exerts%20effective%20in%20vitro%20antiproliferative%20activity%20against%20the%20HT-29%20colon%20cancer%201.pdf
https://eprints.ums.edu.my/id/eprint/33276/
https://www.blacpma.ms-editions.cl/index.php/blacpma/article/view/310/318
https://doi.org/10.37360/blacpma.22.21.5.41
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score 13.211869

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