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Diversity and natural selection of Merozoite surface protein-1 in three species of human malaria parasites: Contribution from South-East Asian isolates

The present study aimed to examine the genetic diversity of human malaria parasites (i.e., P. falciparum, P. vivax and P. knowlesi) in Malaysia and southern Thailand targeting the 19-kDa C-terminal region of Merozoite Surface Protein-1 (MSP-119). This region is essential for the recognition and inva...

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Main Authors: Goh, Xiang Ting, Lim, Yvonne A.L., Lee, Ping Chin, Veeranoot Nissapatorn, Chua, Kek Heng
Format: Article
Language:English
English
Published: Elsevier 2021
Subjects:
QR1-502 Microbiology
Online Access:https://eprints.ums.edu.my/id/eprint/33209/1/Diversity%20and%20natural%20selection%20of%20merozoite%20surface%20protein-1%20in%20three%20species%20of%20human%20malaria%20parasites_%20Contribution%20from%20South-East%20Asian%20isolates.pdf
https://eprints.ums.edu.my/id/eprint/33209/3/Diversity%20and%20natural%20selection%20of%20merozoite%20surface%20protein-1%20in%20three%20species%20of%20human%20malaria%20parasites%20_ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/33209/
https://www.sciencedirect.com/science/article/pii/S0166685121000372?casa_token=PVQnm1aUIREAAAAA:vT5NSOFJWclofotd0qrmoRVg-JbQGExo_ImmYPgOauoNxqCPTZ3GryX6iRMOb6wnyMHVBUQN7n4
https://doi.org/10.1016/j.molbiopara.2021.111390
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spelling my.ums.eprints.332092022-07-16T02:04:10Z https://eprints.ums.edu.my/id/eprint/33209/ Diversity and natural selection of Merozoite surface protein-1 in three species of human malaria parasites: Contribution from South-East Asian isolates Goh, Xiang Ting Lim, Yvonne A.L. Lee, Ping Chin Veeranoot Nissapatorn Chua, Kek Heng QR1-502 Microbiology The present study aimed to examine the genetic diversity of human malaria parasites (i.e., P. falciparum, P. vivax and P. knowlesi) in Malaysia and southern Thailand targeting the 19-kDa C-terminal region of Merozoite Surface Protein-1 (MSP-119). This region is essential for the recognition and invasion of erythrocytes and it is considered one of the leading candidates for asexual blood stage vaccines. However, the genetic data of MSP-119 among human malaria parasites in Malaysia is limited and there is also a need to update the current sequence diversity of this gene region among the Thailand isolates. In this study, genomic DNA was extracted from 384 microscopy-positive blood samples collected from patients who attended the hospitals or clinics in Malaysia and malaria clinics in Thailand from the year 2008 to 2016. The MSP-119 was amplified using PCR followed by bidirectional sequencing. DNA sequences identified in the present study were subjected to Median-joining network analysis with sequences of MSP-119 obtained from GenBank. DNA sequence analysis revealed that PfMSP-119 of Malaysian and Thailand isolates was not genetically conserved as high number of haplotypes were detected and positive selection was prevalent in PfMSP-119, hence questioning its suitability to be used as a vaccine candidate. A novel haplotype (Q/TNG/L) was also detected in Thailand P. falciparum isolate. In contrast, PvMSP-119 was highly conserved, however for the first time, a non-synonymous substitution (A1657S) was reported among Malaysian isolates. As for PkMSP-119, the presence of purifying selection and low nucleotide diversity indicated that it might be a potential vaccine target for P. knowlesi. Elsevier 2021 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/33209/1/Diversity%20and%20natural%20selection%20of%20merozoite%20surface%20protein-1%20in%20three%20species%20of%20human%20malaria%20parasites_%20Contribution%20from%20South-East%20Asian%20isolates.pdf text en https://eprints.ums.edu.my/id/eprint/33209/3/Diversity%20and%20natural%20selection%20of%20merozoite%20surface%20protein-1%20in%20three%20species%20of%20human%20malaria%20parasites%20_ABSTRACT.pdf Goh, Xiang Ting and Lim, Yvonne A.L. and Lee, Ping Chin and Veeranoot Nissapatorn and Chua, Kek Heng (2021) Diversity and natural selection of Merozoite surface protein-1 in three species of human malaria parasites: Contribution from South-East Asian isolates. Molecular and Biochemical Parasitology, 244. pp. 1-12. ISSN 0166-6851 https://www.sciencedirect.com/science/article/pii/S0166685121000372?casa_token=PVQnm1aUIREAAAAA:vT5NSOFJWclofotd0qrmoRVg-JbQGExo_ImmYPgOauoNxqCPTZ3GryX6iRMOb6wnyMHVBUQN7n4 https://doi.org/10.1016/j.molbiopara.2021.111390
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
English
topic QR1-502 Microbiology
spellingShingle QR1-502 Microbiology
Goh, Xiang Ting
Lim, Yvonne A.L.
Lee, Ping Chin
Veeranoot Nissapatorn
Chua, Kek Heng
Diversity and natural selection of Merozoite surface protein-1 in three species of human malaria parasites: Contribution from South-East Asian isolates
description The present study aimed to examine the genetic diversity of human malaria parasites (i.e., P. falciparum, P. vivax and P. knowlesi) in Malaysia and southern Thailand targeting the 19-kDa C-terminal region of Merozoite Surface Protein-1 (MSP-119). This region is essential for the recognition and invasion of erythrocytes and it is considered one of the leading candidates for asexual blood stage vaccines. However, the genetic data of MSP-119 among human malaria parasites in Malaysia is limited and there is also a need to update the current sequence diversity of this gene region among the Thailand isolates. In this study, genomic DNA was extracted from 384 microscopy-positive blood samples collected from patients who attended the hospitals or clinics in Malaysia and malaria clinics in Thailand from the year 2008 to 2016. The MSP-119 was amplified using PCR followed by bidirectional sequencing. DNA sequences identified in the present study were subjected to Median-joining network analysis with sequences of MSP-119 obtained from GenBank. DNA sequence analysis revealed that PfMSP-119 of Malaysian and Thailand isolates was not genetically conserved as high number of haplotypes were detected and positive selection was prevalent in PfMSP-119, hence questioning its suitability to be used as a vaccine candidate. A novel haplotype (Q/TNG/L) was also detected in Thailand P. falciparum isolate. In contrast, PvMSP-119 was highly conserved, however for the first time, a non-synonymous substitution (A1657S) was reported among Malaysian isolates. As for PkMSP-119, the presence of purifying selection and low nucleotide diversity indicated that it might be a potential vaccine target for P. knowlesi.
format Article
author Goh, Xiang Ting
Lim, Yvonne A.L.
Lee, Ping Chin
Veeranoot Nissapatorn
Chua, Kek Heng
author_facet Goh, Xiang Ting
Lim, Yvonne A.L.
Lee, Ping Chin
Veeranoot Nissapatorn
Chua, Kek Heng
author_sort Goh, Xiang Ting
title Diversity and natural selection of Merozoite surface protein-1 in three species of human malaria parasites: Contribution from South-East Asian isolates
title_short Diversity and natural selection of Merozoite surface protein-1 in three species of human malaria parasites: Contribution from South-East Asian isolates
title_full Diversity and natural selection of Merozoite surface protein-1 in three species of human malaria parasites: Contribution from South-East Asian isolates
title_fullStr Diversity and natural selection of Merozoite surface protein-1 in three species of human malaria parasites: Contribution from South-East Asian isolates
title_full_unstemmed Diversity and natural selection of Merozoite surface protein-1 in three species of human malaria parasites: Contribution from South-East Asian isolates
title_sort diversity and natural selection of merozoite surface protein-1 in three species of human malaria parasites: contribution from south-east asian isolates
publisher Elsevier
publishDate 2021
url https://eprints.ums.edu.my/id/eprint/33209/1/Diversity%20and%20natural%20selection%20of%20merozoite%20surface%20protein-1%20in%20three%20species%20of%20human%20malaria%20parasites_%20Contribution%20from%20South-East%20Asian%20isolates.pdf
https://eprints.ums.edu.my/id/eprint/33209/3/Diversity%20and%20natural%20selection%20of%20merozoite%20surface%20protein-1%20in%20three%20species%20of%20human%20malaria%20parasites%20_ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/33209/
https://www.sciencedirect.com/science/article/pii/S0166685121000372?casa_token=PVQnm1aUIREAAAAA:vT5NSOFJWclofotd0qrmoRVg-JbQGExo_ImmYPgOauoNxqCPTZ3GryX6iRMOb6wnyMHVBUQN7n4
https://doi.org/10.1016/j.molbiopara.2021.111390
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