Cover Image

Identification And Occurrence of Antibiotic Resistance of Staphylococcus Aureus and Escherichia Coli Isolated from Recreational Parks Around Kota Kinabalu, Sabah

Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) are contributors to infection cases among the Asian population. S. aureus is found in the mucous lining of noses and is mainly non-pathogenic while E. coli, mostly harmless bacteria, are found in the intestine. Pathogenic strains of bo...

Full description

Saved in:
Bibliographic Details
Main Authors: Rajeena Sugumaran, Pamela David Jocksing, Nur Athirah Yusof
Format: Article
Language:English
English
Published: Universiti Malaysia Sabah 2020
Subjects:
QR75-99.5 Bacteria
RA1-1270 Public aspects of medicine
Online Access:https://eprints.ums.edu.my/id/eprint/33094/1/Identification%20And%20Occurrence%20of%20Antibiotic%20Resistance%20of%20Staphylococcus%20Aureus%20and%20Escherichia%20Coli%20Isolated%20from%20Recreational%20Parks%20Around%20Kota%20Kinabalu%2C%20Sabah.pdf
https://eprints.ums.edu.my/id/eprint/33094/2/Identification%20And%20Occurrence%20of%20Antibiotic%20Resistance%20of%20Staphylococcus%20Aureus%20and%20Escherichia%20Coli%20Isolated%20from%20Recreational%20Parks%20Around%20Kota%20Kinabalu%2C%20Sabah1.pdf
https://eprints.ums.edu.my/id/eprint/33094/
https://jurcon.ums.edu.my/ojums/index.php/bijb/article/view/1975
https://doi.org/10.51200/bijb.vi.1975
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.ums.eprints.33094
record_format eprints
spelling my.ums.eprints.330942022-07-12T00:55:33Z https://eprints.ums.edu.my/id/eprint/33094/ Identification And Occurrence of Antibiotic Resistance of Staphylococcus Aureus and Escherichia Coli Isolated from Recreational Parks Around Kota Kinabalu, Sabah Rajeena Sugumaran Pamela David Jocksing Nur Athirah Yusof QR75-99.5 Bacteria RA1-1270 Public aspects of medicine Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) are contributors to infection cases among the Asian population. S. aureus is found in the mucous lining of noses and is mainly non-pathogenic while E. coli, mostly harmless bacteria, are found in the intestine. Pathogenic strains of both bacteria have adverse effects on the elderly and younger age group of the population. Samples were collected from recreational parks around Kota Kinabalu as they are hotspots frequently visited by families with both age groups. The bacterial samples were isolated and cultured on selective media such as Baird-Parker agar (BPA), Brain Heart Infusion (BHI) agar, MacConkey agar and Eosin-Methylene Blue (EMB) agar. Morphological characteristics of bacterial growth were observed, where S. aureus had black-shiny growth in BPA and E. coli had a metallic-green sheen in EMB agar. The suspected bacteria samples were then stained and viewed under a light microscope. S. aureus was identified as gram-positive, stained violet with a circular shape and clustered appearance. E. coli was identified as gram-negative, stained red, rod-shaped with 2 – 3 bacterial alignments. Antibiotic resistance test resulted in S. aureus and E. coli samples did not display 100% resistance among 4 antibiotics tested (ampicillin, penicillin, tetracycline and chloramphenicol). Most of the bacteria samples were a minimum inhibitory of 0.1 mg/mL of antibiotic concentration. These results provide a foundation for further research on identifying bacterial strains using molecular methods. The findings can then be used to disseminate information to the public to create awareness of potential disease outbreaks in the city. Universiti Malaysia Sabah 2020 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/33094/1/Identification%20And%20Occurrence%20of%20Antibiotic%20Resistance%20of%20Staphylococcus%20Aureus%20and%20Escherichia%20Coli%20Isolated%20from%20Recreational%20Parks%20Around%20Kota%20Kinabalu%2C%20Sabah.pdf text en https://eprints.ums.edu.my/id/eprint/33094/2/Identification%20And%20Occurrence%20of%20Antibiotic%20Resistance%20of%20Staphylococcus%20Aureus%20and%20Escherichia%20Coli%20Isolated%20from%20Recreational%20Parks%20Around%20Kota%20Kinabalu%2C%20Sabah1.pdf Rajeena Sugumaran and Pamela David Jocksing and Nur Athirah Yusof (2020) Identification And Occurrence of Antibiotic Resistance of Staphylococcus Aureus and Escherichia Coli Isolated from Recreational Parks Around Kota Kinabalu, Sabah. Borneo International Journal of Biotechnology (BIJB), 1. pp. 55-75. ISSN 2716-697X https://jurcon.ums.edu.my/ojums/index.php/bijb/article/view/1975 https://doi.org/10.51200/bijb.vi.1975
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
English
topic QR75-99.5 Bacteria
RA1-1270 Public aspects of medicine
spellingShingle QR75-99.5 Bacteria
RA1-1270 Public aspects of medicine
Rajeena Sugumaran
Pamela David Jocksing
Nur Athirah Yusof
Identification And Occurrence of Antibiotic Resistance of Staphylococcus Aureus and Escherichia Coli Isolated from Recreational Parks Around Kota Kinabalu, Sabah
description Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) are contributors to infection cases among the Asian population. S. aureus is found in the mucous lining of noses and is mainly non-pathogenic while E. coli, mostly harmless bacteria, are found in the intestine. Pathogenic strains of both bacteria have adverse effects on the elderly and younger age group of the population. Samples were collected from recreational parks around Kota Kinabalu as they are hotspots frequently visited by families with both age groups. The bacterial samples were isolated and cultured on selective media such as Baird-Parker agar (BPA), Brain Heart Infusion (BHI) agar, MacConkey agar and Eosin-Methylene Blue (EMB) agar. Morphological characteristics of bacterial growth were observed, where S. aureus had black-shiny growth in BPA and E. coli had a metallic-green sheen in EMB agar. The suspected bacteria samples were then stained and viewed under a light microscope. S. aureus was identified as gram-positive, stained violet with a circular shape and clustered appearance. E. coli was identified as gram-negative, stained red, rod-shaped with 2 – 3 bacterial alignments. Antibiotic resistance test resulted in S. aureus and E. coli samples did not display 100% resistance among 4 antibiotics tested (ampicillin, penicillin, tetracycline and chloramphenicol). Most of the bacteria samples were a minimum inhibitory of 0.1 mg/mL of antibiotic concentration. These results provide a foundation for further research on identifying bacterial strains using molecular methods. The findings can then be used to disseminate information to the public to create awareness of potential disease outbreaks in the city.
format Article
author Rajeena Sugumaran
Pamela David Jocksing
Nur Athirah Yusof
author_facet Rajeena Sugumaran
Pamela David Jocksing
Nur Athirah Yusof
author_sort Rajeena Sugumaran
title Identification And Occurrence of Antibiotic Resistance of Staphylococcus Aureus and Escherichia Coli Isolated from Recreational Parks Around Kota Kinabalu, Sabah
title_short Identification And Occurrence of Antibiotic Resistance of Staphylococcus Aureus and Escherichia Coli Isolated from Recreational Parks Around Kota Kinabalu, Sabah
title_full Identification And Occurrence of Antibiotic Resistance of Staphylococcus Aureus and Escherichia Coli Isolated from Recreational Parks Around Kota Kinabalu, Sabah
title_fullStr Identification And Occurrence of Antibiotic Resistance of Staphylococcus Aureus and Escherichia Coli Isolated from Recreational Parks Around Kota Kinabalu, Sabah
title_full_unstemmed Identification And Occurrence of Antibiotic Resistance of Staphylococcus Aureus and Escherichia Coli Isolated from Recreational Parks Around Kota Kinabalu, Sabah
title_sort identification and occurrence of antibiotic resistance of staphylococcus aureus and escherichia coli isolated from recreational parks around kota kinabalu, sabah
publisher Universiti Malaysia Sabah
publishDate 2020
url https://eprints.ums.edu.my/id/eprint/33094/1/Identification%20And%20Occurrence%20of%20Antibiotic%20Resistance%20of%20Staphylococcus%20Aureus%20and%20Escherichia%20Coli%20Isolated%20from%20Recreational%20Parks%20Around%20Kota%20Kinabalu%2C%20Sabah.pdf
https://eprints.ums.edu.my/id/eprint/33094/2/Identification%20And%20Occurrence%20of%20Antibiotic%20Resistance%20of%20Staphylococcus%20Aureus%20and%20Escherichia%20Coli%20Isolated%20from%20Recreational%20Parks%20Around%20Kota%20Kinabalu%2C%20Sabah1.pdf
https://eprints.ums.edu.my/id/eprint/33094/
https://jurcon.ums.edu.my/ojums/index.php/bijb/article/view/1975
https://doi.org/10.51200/bijb.vi.1975
_version_ 1760231116859506688
score 13.211869

Similar Items