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Growth and utilization of oil sludge as a source of carbon by locally isolated beneficial microorganisms

Recent advance technologies have showed that microorganisms plays an important role in the natural ecosystem and act as bio-control of the environment. As microorganisms have become more concern in environment technology, the understanding of microorganism’s life cycle and its factor influencing its...

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Main Authors: Nur Zaida Zahari, Fera @ Nony Cleophas, Siti Aishah Mohd Ali, S. Abd Rahim
Format: Proceedings
Language:English
English
Published: Faculty of Science and Natural Resources 2021
Subjects:
QR1-502 Microbiology
Online Access:https://eprints.ums.edu.my/id/eprint/32064/1/Growth%20and%20utilization%20of%20oil%20sludge%20as%20a%20source%20of%20carbon%20by%20locally%20isolated%20beneficial%20microorganisms.ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/32064/2/GROWTH%20AND%20UTILIZATION%20OF%20OIL%20SLUDGE%20AS%20A%20SOURCE%20OF%20CARBON%20BY%20LOCALLY%20ISOLATED%20BENEFICIAL%20MICROORGANISMS.pdf
https://eprints.ums.edu.my/id/eprint/32064/
https://drive.google977-2-73-522200-2.com/file/d/1zKEpSiYvg9vZHHzuKra49512D_AMLubu/view
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spelling my.ums.eprints.320642022-03-31T00:01:15Z https://eprints.ums.edu.my/id/eprint/32064/ Growth and utilization of oil sludge as a source of carbon by locally isolated beneficial microorganisms Nur Zaida Zahari Fera @ Nony Cleophas Siti Aishah Mohd Ali S. Abd Rahim QR1-502 Microbiology Recent advance technologies have showed that microorganisms plays an important role in the natural ecosystem and act as bio-control of the environment. As microorganisms have become more concern in environment technology, the understanding of microorganism’s life cycle and its factor influencing its life span must fully understand. Therefore, in this study, three isolated beneficial microorganisms identified as Chromobacterium violeceum- MAB-Cr1, Pseudomonas aeruginosa- BAS-Cr1 and Stenotrophomonas maltophilia-RAS-Cr1 was used to identify the growth phase in utilization of oil sludge at different concentration levels. These microorganisms were isolated from Titan Petrochemical, Pasir Gudang Johor and have been proven to degrade phenol based on the previous study done. The experiment was conducted in a conical flasks with addition of different concentrations of oil sludge at 5%, 10%, 15% v/v as a sole carbon. After 24 hours of incubation, the growth of microorganisms were assessed using spectrophotometer at 600nm and the standard growth profile were plotted according to the concentrations studied. The results showed that all strains exhibited lag, exponential, stationary and death phase in oil sludge utilization. The maximum optical density (OD) was observed in C. violaceum-MAB-Cr1 (0.85; 10% v/v), P. aeruginosa-BAS-Cr1 (0.82; 10% v/v) and S. maltophilia-RAS-Cr1 (0.91; 15% v/v). It can be concludes that as the highest concentration of oil sludge present in the medium, the longest survival rate recorded for all strains studied. This findings proved that all strains have capability and potential to tolerate in high concentration of oil sludge. Faculty of Science and Natural Resources 2021-10 Proceedings PeerReviewed text en https://eprints.ums.edu.my/id/eprint/32064/1/Growth%20and%20utilization%20of%20oil%20sludge%20as%20a%20source%20of%20carbon%20by%20locally%20isolated%20beneficial%20microorganisms.ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/32064/2/GROWTH%20AND%20UTILIZATION%20OF%20OIL%20SLUDGE%20AS%20A%20SOURCE%20OF%20CARBON%20BY%20LOCALLY%20ISOLATED%20BENEFICIAL%20MICROORGANISMS.pdf Nur Zaida Zahari and Fera @ Nony Cleophas and Siti Aishah Mohd Ali and S. Abd Rahim (2021) Growth and utilization of oil sludge as a source of carbon by locally isolated beneficial microorganisms. https://drive.google977-2-73-522200-2.com/file/d/1zKEpSiYvg9vZHHzuKra49512D_AMLubu/view
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
English
topic QR1-502 Microbiology
spellingShingle QR1-502 Microbiology
Nur Zaida Zahari
Fera @ Nony Cleophas
Siti Aishah Mohd Ali
S. Abd Rahim
Growth and utilization of oil sludge as a source of carbon by locally isolated beneficial microorganisms
description Recent advance technologies have showed that microorganisms plays an important role in the natural ecosystem and act as bio-control of the environment. As microorganisms have become more concern in environment technology, the understanding of microorganism’s life cycle and its factor influencing its life span must fully understand. Therefore, in this study, three isolated beneficial microorganisms identified as Chromobacterium violeceum- MAB-Cr1, Pseudomonas aeruginosa- BAS-Cr1 and Stenotrophomonas maltophilia-RAS-Cr1 was used to identify the growth phase in utilization of oil sludge at different concentration levels. These microorganisms were isolated from Titan Petrochemical, Pasir Gudang Johor and have been proven to degrade phenol based on the previous study done. The experiment was conducted in a conical flasks with addition of different concentrations of oil sludge at 5%, 10%, 15% v/v as a sole carbon. After 24 hours of incubation, the growth of microorganisms were assessed using spectrophotometer at 600nm and the standard growth profile were plotted according to the concentrations studied. The results showed that all strains exhibited lag, exponential, stationary and death phase in oil sludge utilization. The maximum optical density (OD) was observed in C. violaceum-MAB-Cr1 (0.85; 10% v/v), P. aeruginosa-BAS-Cr1 (0.82; 10% v/v) and S. maltophilia-RAS-Cr1 (0.91; 15% v/v). It can be concludes that as the highest concentration of oil sludge present in the medium, the longest survival rate recorded for all strains studied. This findings proved that all strains have capability and potential to tolerate in high concentration of oil sludge.
format Proceedings
author Nur Zaida Zahari
Fera @ Nony Cleophas
Siti Aishah Mohd Ali
S. Abd Rahim
author_facet Nur Zaida Zahari
Fera @ Nony Cleophas
Siti Aishah Mohd Ali
S. Abd Rahim
author_sort Nur Zaida Zahari
title Growth and utilization of oil sludge as a source of carbon by locally isolated beneficial microorganisms
title_short Growth and utilization of oil sludge as a source of carbon by locally isolated beneficial microorganisms
title_full Growth and utilization of oil sludge as a source of carbon by locally isolated beneficial microorganisms
title_fullStr Growth and utilization of oil sludge as a source of carbon by locally isolated beneficial microorganisms
title_full_unstemmed Growth and utilization of oil sludge as a source of carbon by locally isolated beneficial microorganisms
title_sort growth and utilization of oil sludge as a source of carbon by locally isolated beneficial microorganisms
publisher Faculty of Science and Natural Resources
publishDate 2021
url https://eprints.ums.edu.my/id/eprint/32064/1/Growth%20and%20utilization%20of%20oil%20sludge%20as%20a%20source%20of%20carbon%20by%20locally%20isolated%20beneficial%20microorganisms.ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/32064/2/GROWTH%20AND%20UTILIZATION%20OF%20OIL%20SLUDGE%20AS%20A%20SOURCE%20OF%20CARBON%20BY%20LOCALLY%20ISOLATED%20BENEFICIAL%20MICROORGANISMS.pdf
https://eprints.ums.edu.my/id/eprint/32064/
https://drive.google977-2-73-522200-2.com/file/d/1zKEpSiYvg9vZHHzuKra49512D_AMLubu/view
_version_ 1760230975626805248
score 13.211869

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