Staff View: Concentrated specimen smear microscopy utilising a polymer membrane sandwich filtration vessel for the detection of acid-fast bacilli in health facilities in Sabah, East Malaysia

Concentrated specimen smear microscopy utilising a polymer membrane sandwich filtration vessel for the detection of acid-fast bacilli in health facilities in Sabah, East Malaysia

A simple, ready-to-use concentrated specimen smear microscopy method employing a nanometer silicon polyvinylidene fluoride (PVDF) polymer membrane sandwich filtration vessel to concentrate acid-fast bacilli (AFB) in samples (SFV-CSSM, Hunan-Tech New Medical System Co. Ltd. China) was compared with d...

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Main Authors:	Dawn Carmel Paul, Yun Fong Ngeow, Sook Fan Yap, Jiloris F. Dony, Richard Avoi, Rashidah Mohammad, Hien Fuh Ng
Format:	Article
Language:	English English
Published:	Elsevier Ltd. 2022
Subjects:	RA1-1270 Public aspects of medicine
Online Access:	https://eprints.ums.edu.my/id/eprint/31876/1/Concentrated%20specimen%20smear%20microscopy%20utilising%20a%20polymer%20membrane%20.ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/31876/2/Concentrated%20specimen%20smear%20microscopy%20utilising%20a%20polymer%20membrane.pdf https://eprints.ums.edu.my/id/eprint/31876/ https://www.sciencedirect.com/science/article/abs/pii/S1472979222000208 https://doi.org/10.1016/j.tube.2022.102183
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id	my.ums.eprints.31876
record_format	eprints
spelling	my.ums.eprints.318762022-03-24T07:21:37Z https://eprints.ums.edu.my/id/eprint/31876/ Concentrated specimen smear microscopy utilising a polymer membrane sandwich filtration vessel for the detection of acid-fast bacilli in health facilities in Sabah, East Malaysia Dawn Carmel Paul Yun Fong Ngeow Sook Fan Yap Jiloris F. Dony Richard Avoi Rashidah Mohammad Hien Fuh Ng RA1-1270 Public aspects of medicine A simple, ready-to-use concentrated specimen smear microscopy method employing a nanometer silicon polyvinylidene fluoride (PVDF) polymer membrane sandwich filtration vessel to concentrate acid-fast bacilli (AFB) in samples (SFV-CSSM, Hunan-Tech New Medical System Co. Ltd. China) was compared with direct sputum smear microscopy (DSSM) to determine its performance using culture on modified Ogawa agar as reference. The results for 4114 clinical samples collected from health facilities in Sabah were interpreted with reference to culture results, sample collection-transportation conditions and clinical data including responses to anti-TB drug treatment. The SFV-CSSM showed higher sensitivity than DSSM (79.4% versus 60.5%) and less background interference. Its ability to detect low levels of AFB at an affordable cost makes it an excellent tool for the screening of pauci-bacillary samples as well as for active case finding in TB control programs. Elsevier Ltd. 2022-02-13 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/31876/1/Concentrated%20specimen%20smear%20microscopy%20utilising%20a%20polymer%20membrane%20.ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/31876/2/Concentrated%20specimen%20smear%20microscopy%20utilising%20a%20polymer%20membrane.pdf Dawn Carmel Paul and Yun Fong Ngeow and Sook Fan Yap and Jiloris F. Dony and Richard Avoi and Rashidah Mohammad and Hien Fuh Ng (2022) Concentrated specimen smear microscopy utilising a polymer membrane sandwich filtration vessel for the detection of acid-fast bacilli in health facilities in Sabah, East Malaysia. Tuberculosis, 133. pp. 1-6. ISSN 1472-9792 https://www.sciencedirect.com/science/article/abs/pii/S1472979222000208 https://doi.org/10.1016/j.tube.2022.102183
institution	Universiti Malaysia Sabah
building	UMS Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaysia Sabah
content_source	UMS Institutional Repository
url_provider	http://eprints.ums.edu.my/
language	English English
topic	RA1-1270 Public aspects of medicine
spellingShingle	RA1-1270 Public aspects of medicine Dawn Carmel Paul Yun Fong Ngeow Sook Fan Yap Jiloris F. Dony Richard Avoi Rashidah Mohammad Hien Fuh Ng Concentrated specimen smear microscopy utilising a polymer membrane sandwich filtration vessel for the detection of acid-fast bacilli in health facilities in Sabah, East Malaysia
description	A simple, ready-to-use concentrated specimen smear microscopy method employing a nanometer silicon polyvinylidene fluoride (PVDF) polymer membrane sandwich filtration vessel to concentrate acid-fast bacilli (AFB) in samples (SFV-CSSM, Hunan-Tech New Medical System Co. Ltd. China) was compared with direct sputum smear microscopy (DSSM) to determine its performance using culture on modified Ogawa agar as reference. The results for 4114 clinical samples collected from health facilities in Sabah were interpreted with reference to culture results, sample collection-transportation conditions and clinical data including responses to anti-TB drug treatment. The SFV-CSSM showed higher sensitivity than DSSM (79.4% versus 60.5%) and less background interference. Its ability to detect low levels of AFB at an affordable cost makes it an excellent tool for the screening of pauci-bacillary samples as well as for active case finding in TB control programs.
format	Article
author	Dawn Carmel Paul Yun Fong Ngeow Sook Fan Yap Jiloris F. Dony Richard Avoi Rashidah Mohammad Hien Fuh Ng
author_facet	Dawn Carmel Paul Yun Fong Ngeow Sook Fan Yap Jiloris F. Dony Richard Avoi Rashidah Mohammad Hien Fuh Ng
author_sort	Dawn Carmel Paul
title	Concentrated specimen smear microscopy utilising a polymer membrane sandwich filtration vessel for the detection of acid-fast bacilli in health facilities in Sabah, East Malaysia
title_short	Concentrated specimen smear microscopy utilising a polymer membrane sandwich filtration vessel for the detection of acid-fast bacilli in health facilities in Sabah, East Malaysia
title_full	Concentrated specimen smear microscopy utilising a polymer membrane sandwich filtration vessel for the detection of acid-fast bacilli in health facilities in Sabah, East Malaysia
title_fullStr	Concentrated specimen smear microscopy utilising a polymer membrane sandwich filtration vessel for the detection of acid-fast bacilli in health facilities in Sabah, East Malaysia
title_full_unstemmed	Concentrated specimen smear microscopy utilising a polymer membrane sandwich filtration vessel for the detection of acid-fast bacilli in health facilities in Sabah, East Malaysia
title_sort	concentrated specimen smear microscopy utilising a polymer membrane sandwich filtration vessel for the detection of acid-fast bacilli in health facilities in sabah, east malaysia
publisher	Elsevier Ltd.
publishDate	2022
url	https://eprints.ums.edu.my/id/eprint/31876/1/Concentrated%20specimen%20smear%20microscopy%20utilising%20a%20polymer%20membrane%20.ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/31876/2/Concentrated%20specimen%20smear%20microscopy%20utilising%20a%20polymer%20membrane.pdf https://eprints.ums.edu.my/id/eprint/31876/ https://www.sciencedirect.com/science/article/abs/pii/S1472979222000208 https://doi.org/10.1016/j.tube.2022.102183
_version_	1760230950525992960
score	13.211869

Concentrated specimen smear microscopy utilising a polymer membrane sandwich filtration vessel for the detection of acid-fast bacilli in health facilities in Sabah, East Malaysia

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