Staff View: The fungal diversity of Madai Cave, Sabah, Malaysia

The fungal diversity of Madai Cave, Sabah, Malaysia

Borneo is one of the biodiversity hotspots of the world, but its limestone caves are one of its least studied ecosystems. Studies of the cave mycobiota of Sabah is essentially nonexistent. In this study, speleothem (n = 4), cavern water (n =4), and bat guano (n = 4) samples were collected and studie...

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Main Authors:	Ibrahem G. Wasti, Jaya Seelan Sathiya Seelan
Format:	Proceedings
Language:	English English
Published:	Asian Scholars Network 2019
Subjects:	DS597.33-597.34 Sabah. British North Borneo QH540-549.5 Ecology QK1-989 Botany
Online Access:	https://eprints.ums.edu.my/id/eprint/31146/1/The%20fungal%20diversity%20of%20Madai%20Cave%2C%20Sabah%2C%20Malaysia-ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/31146/2/The%20Fungal%20Diversity%20Of%20Madai%20Cave%2C%20Sabah%2C%20Malaysia.pdf https://eprints.ums.edu.my/id/eprint/31146/ https://www.researchgate.net/publication/338606985_LIMAC_2019_Conference_Proceeding_1
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spelling	my.ums.eprints.311462021-11-23T01:35:42Z https://eprints.ums.edu.my/id/eprint/31146/ The fungal diversity of Madai Cave, Sabah, Malaysia Ibrahem G. Wasti Jaya Seelan Sathiya Seelan DS597.33-597.34 Sabah. British North Borneo QH540-549.5 Ecology QK1-989 Botany Borneo is one of the biodiversity hotspots of the world, but its limestone caves are one of its least studied ecosystems. Studies of the cave mycobiota of Sabah is essentially nonexistent. In this study, speleothem (n = 4), cavern water (n =4), and bat guano (n = 4) samples were collected and studied for their fungal diversity and abundance. Opportunistic sampling was utilized and the dilution method was performed during isolation. Fifty-five culturable fungi were isolated of which 32 species from 15 genera, nine orders, and two phyla were observed. Morphological characterization and molecular analysis of the ITS gene region were utilized for identification of the isolates. The average fungal abundance count for speleothem samples were 229.3 CFU/cm2, cavern water was 335.0 CFU/ml, and bat guano was 6266.7 CFU/g. Simpson and Shannon diversity indices indicated that speleothem samples had the highest fungal diversity, followed by bat guano, and cavern water. Speleothem also resulted in the most pure isolates (n=23) and distinct fungal taxa (n=19). Ascomycetes dominated the fungal composition of all sample types, accounting for 53 out of 55 total isolates. The remaining two isolates were both Basidiomycetes. The most abundant genera recovered from cave samples was Penicillium, but Aspergillus spp. had the highest occurrence as they were isolated from all samples except for one. Purpureocillium lilacinum was the species with the highest occurrence, appearing in five separate samples from all three substrate types. This study serves to produce baseline data useful for further research on the mycoflora of Sabah’s various ecosystems. We urge that visitors should be more aware of the potential risks and disturbance they cause to microbial communities when entering cave environments. Asian Scholars Network 2019 Proceedings PeerReviewed text en https://eprints.ums.edu.my/id/eprint/31146/1/The%20fungal%20diversity%20of%20Madai%20Cave%2C%20Sabah%2C%20Malaysia-ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/31146/2/The%20Fungal%20Diversity%20Of%20Madai%20Cave%2C%20Sabah%2C%20Malaysia.pdf Ibrahem G. Wasti and Jaya Seelan Sathiya Seelan (2019) The fungal diversity of Madai Cave, Sabah, Malaysia. https://www.researchgate.net/publication/338606985_LIMAC_2019_Conference_Proceeding_1
institution	Universiti Malaysia Sabah
building	UMS Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaysia Sabah
content_source	UMS Institutional Repository
url_provider	http://eprints.ums.edu.my/
language	English English
topic	DS597.33-597.34 Sabah. British North Borneo QH540-549.5 Ecology QK1-989 Botany
spellingShingle	DS597.33-597.34 Sabah. British North Borneo QH540-549.5 Ecology QK1-989 Botany Ibrahem G. Wasti Jaya Seelan Sathiya Seelan The fungal diversity of Madai Cave, Sabah, Malaysia
description	Borneo is one of the biodiversity hotspots of the world, but its limestone caves are one of its least studied ecosystems. Studies of the cave mycobiota of Sabah is essentially nonexistent. In this study, speleothem (n = 4), cavern water (n =4), and bat guano (n = 4) samples were collected and studied for their fungal diversity and abundance. Opportunistic sampling was utilized and the dilution method was performed during isolation. Fifty-five culturable fungi were isolated of which 32 species from 15 genera, nine orders, and two phyla were observed. Morphological characterization and molecular analysis of the ITS gene region were utilized for identification of the isolates. The average fungal abundance count for speleothem samples were 229.3 CFU/cm2, cavern water was 335.0 CFU/ml, and bat guano was 6266.7 CFU/g. Simpson and Shannon diversity indices indicated that speleothem samples had the highest fungal diversity, followed by bat guano, and cavern water. Speleothem also resulted in the most pure isolates (n=23) and distinct fungal taxa (n=19). Ascomycetes dominated the fungal composition of all sample types, accounting for 53 out of 55 total isolates. The remaining two isolates were both Basidiomycetes. The most abundant genera recovered from cave samples was Penicillium, but Aspergillus spp. had the highest occurrence as they were isolated from all samples except for one. Purpureocillium lilacinum was the species with the highest occurrence, appearing in five separate samples from all three substrate types. This study serves to produce baseline data useful for further research on the mycoflora of Sabah’s various ecosystems. We urge that visitors should be more aware of the potential risks and disturbance they cause to microbial communities when entering cave environments.
format	Proceedings
author	Ibrahem G. Wasti Jaya Seelan Sathiya Seelan
author_facet	Ibrahem G. Wasti Jaya Seelan Sathiya Seelan
author_sort	Ibrahem G. Wasti
title	The fungal diversity of Madai Cave, Sabah, Malaysia
title_short	The fungal diversity of Madai Cave, Sabah, Malaysia
title_full	The fungal diversity of Madai Cave, Sabah, Malaysia
title_fullStr	The fungal diversity of Madai Cave, Sabah, Malaysia
title_full_unstemmed	The fungal diversity of Madai Cave, Sabah, Malaysia
title_sort	fungal diversity of madai cave, sabah, malaysia
publisher	Asian Scholars Network
publishDate	2019
url	https://eprints.ums.edu.my/id/eprint/31146/1/The%20fungal%20diversity%20of%20Madai%20Cave%2C%20Sabah%2C%20Malaysia-ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/31146/2/The%20Fungal%20Diversity%20Of%20Madai%20Cave%2C%20Sabah%2C%20Malaysia.pdf https://eprints.ums.edu.my/id/eprint/31146/ https://www.researchgate.net/publication/338606985_LIMAC_2019_Conference_Proceeding_1
_version_	1760230856281030656
score	13.211869

The fungal diversity of Madai Cave, Sabah, Malaysia

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