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Characterization of bacteriocin and chitinase producing bacterial isolates with broad-spectrum antimicrobial activities

There is a need for more efficient and eco-friendly approaches to overcome increasing microbial infections. Bacteriocins and chitinases from Bacillus spp. can be powerful alternatives to conventional antibiotics and antifungal drugs, respectively. The purpose of this study was to assess the inhibito...

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Main Authors: Muhammad Yasir, Basit Zeshan, Nur Hardy A. Daud, Izzah Shahid, Hafza Khalid
Format: Article
Language:English
English
Published: Springer Nature 2021
Subjects:
QK710-899 Plant physiology
QR75-99.5 Bacteria
Online Access:https://eprints.ums.edu.my/id/eprint/30977/1/Characterization%20of%20bacteriocin%20and%20chitinase%20producing%20bacterial%20isolates%20with%20broad-spectrum%20antimicrobial%20activities-ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/30977/2/Characterization%20of%20bacteriocin%20and%20chitinase%20producing%20bacterial%20isolates%20with%20broad-spectrum%20antimicrobial%20activities.pdf
https://eprints.ums.edu.my/id/eprint/30977/
https://link.springer.com/article/10.1007/s42452-021-04740-z
https://doi.org/10.1007/s42452-021-04740-z
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spelling my.ums.eprints.309772021-11-19T08:45:58Z https://eprints.ums.edu.my/id/eprint/30977/ Characterization of bacteriocin and chitinase producing bacterial isolates with broad-spectrum antimicrobial activities Muhammad Yasir Basit Zeshan Nur Hardy A. Daud Izzah Shahid Hafza Khalid QK710-899 Plant physiology QR75-99.5 Bacteria There is a need for more efficient and eco-friendly approaches to overcome increasing microbial infections. Bacteriocins and chitinases from Bacillus spp. can be powerful alternatives to conventional antibiotics and antifungal drugs, respectively. The purpose of this study was to assess the inhibitory potential of bacteriocins and chitinase enzymes against multiple resistant bacterial and fungal pathogens. Bacterial isolates were selected by growth on minimal salts medium and after that were morphologically and biochemically characterized. The physiochemical characterization of bacteriocins was carried out. The inhibitory potential of bacteriocins towards six pathogenic bacteria was determined by the well diffusion assay while chitinase activity towards three fungal strains was determined by the dual plate culture assay. Two bacterial strains ( WW2P1 and WRE4P2), out of nine showed inhibition of K. pneumonia, P. aeruginosa, E. coli and MRSA while WW4P2 was positive against S. typhimurium and E. coli and WRE10P2 against P. aeruginosa, S. pneumoniae. Two bacterial isolates ( WW3P1 and WRE10P2) were chosen for further study on the basis of their antifungal activities. Of these, WW3P1 isolate was more effective against A. fumigatus as well as A. niger. The proteinaceous nature of the bacteriocins was confirmed by treatment of the crude extract with proteinase K. It was found that the inhibitory activity of strain WW3P1 against E. coli was highest at 20 °C, and against S. pneumoniae it was at 20 °C and pH 10 after treatment with EDTA. Inhibition by strain the WRE10P2 against P. aeruginosa was highest at 20 °C and pH 14. It was found that EDTA increased the inhibitory activity of strain WW2P1 against P. aeruginosa, K. pneumoniae and E. coli by 2 ± 0.235, 3.5 ± 0.288, 2.5 ± 1.040 times, respectively, of strain WRE4P2 against P. aeruginosa and E. coli by 2.5 ± 0.763, 2.7 ± 0.5 times, respectively, and of strain WRE10P2 against S. pneumoniae by 3 ± 0.6236 times. The isolates have promising inhibitory activity, which should be further analyzed for the commercial production of antimicrobials. Springer Nature 2021-07-28 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/30977/1/Characterization%20of%20bacteriocin%20and%20chitinase%20producing%20bacterial%20isolates%20with%20broad-spectrum%20antimicrobial%20activities-ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/30977/2/Characterization%20of%20bacteriocin%20and%20chitinase%20producing%20bacterial%20isolates%20with%20broad-spectrum%20antimicrobial%20activities.pdf Muhammad Yasir and Basit Zeshan and Nur Hardy A. Daud and Izzah Shahid and Hafza Khalid (2021) Characterization of bacteriocin and chitinase producing bacterial isolates with broad-spectrum antimicrobial activities. Springer Nature Applied Sciences, 3 (755). ISSN 2523-3971 https://link.springer.com/article/10.1007/s42452-021-04740-z https://doi.org/10.1007/s42452-021-04740-z
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
English
topic QK710-899 Plant physiology
QR75-99.5 Bacteria
spellingShingle QK710-899 Plant physiology
QR75-99.5 Bacteria
Muhammad Yasir
Basit Zeshan
Nur Hardy A. Daud
Izzah Shahid
Hafza Khalid
Characterization of bacteriocin and chitinase producing bacterial isolates with broad-spectrum antimicrobial activities
description There is a need for more efficient and eco-friendly approaches to overcome increasing microbial infections. Bacteriocins and chitinases from Bacillus spp. can be powerful alternatives to conventional antibiotics and antifungal drugs, respectively. The purpose of this study was to assess the inhibitory potential of bacteriocins and chitinase enzymes against multiple resistant bacterial and fungal pathogens. Bacterial isolates were selected by growth on minimal salts medium and after that were morphologically and biochemically characterized. The physiochemical characterization of bacteriocins was carried out. The inhibitory potential of bacteriocins towards six pathogenic bacteria was determined by the well diffusion assay while chitinase activity towards three fungal strains was determined by the dual plate culture assay. Two bacterial strains ( WW2P1 and WRE4P2), out of nine showed inhibition of K. pneumonia, P. aeruginosa, E. coli and MRSA while WW4P2 was positive against S. typhimurium and E. coli and WRE10P2 against P. aeruginosa, S. pneumoniae. Two bacterial isolates ( WW3P1 and WRE10P2) were chosen for further study on the basis of their antifungal activities. Of these, WW3P1 isolate was more effective against A. fumigatus as well as A. niger. The proteinaceous nature of the bacteriocins was confirmed by treatment of the crude extract with proteinase K. It was found that the inhibitory activity of strain WW3P1 against E. coli was highest at 20 °C, and against S. pneumoniae it was at 20 °C and pH 10 after treatment with EDTA. Inhibition by strain the WRE10P2 against P. aeruginosa was highest at 20 °C and pH 14. It was found that EDTA increased the inhibitory activity of strain WW2P1 against P. aeruginosa, K. pneumoniae and E. coli by 2 ± 0.235, 3.5 ± 0.288, 2.5 ± 1.040 times, respectively, of strain WRE4P2 against P. aeruginosa and E. coli by 2.5 ± 0.763, 2.7 ± 0.5 times, respectively, and of strain WRE10P2 against S. pneumoniae by 3 ± 0.6236 times. The isolates have promising inhibitory activity, which should be further analyzed for the commercial production of antimicrobials.
format Article
author Muhammad Yasir
Basit Zeshan
Nur Hardy A. Daud
Izzah Shahid
Hafza Khalid
author_facet Muhammad Yasir
Basit Zeshan
Nur Hardy A. Daud
Izzah Shahid
Hafza Khalid
author_sort Muhammad Yasir
title Characterization of bacteriocin and chitinase producing bacterial isolates with broad-spectrum antimicrobial activities
title_short Characterization of bacteriocin and chitinase producing bacterial isolates with broad-spectrum antimicrobial activities
title_full Characterization of bacteriocin and chitinase producing bacterial isolates with broad-spectrum antimicrobial activities
title_fullStr Characterization of bacteriocin and chitinase producing bacterial isolates with broad-spectrum antimicrobial activities
title_full_unstemmed Characterization of bacteriocin and chitinase producing bacterial isolates with broad-spectrum antimicrobial activities
title_sort characterization of bacteriocin and chitinase producing bacterial isolates with broad-spectrum antimicrobial activities
publisher Springer Nature
publishDate 2021
url https://eprints.ums.edu.my/id/eprint/30977/1/Characterization%20of%20bacteriocin%20and%20chitinase%20producing%20bacterial%20isolates%20with%20broad-spectrum%20antimicrobial%20activities-ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/30977/2/Characterization%20of%20bacteriocin%20and%20chitinase%20producing%20bacterial%20isolates%20with%20broad-spectrum%20antimicrobial%20activities.pdf
https://eprints.ums.edu.my/id/eprint/30977/
https://link.springer.com/article/10.1007/s42452-021-04740-z
https://doi.org/10.1007/s42452-021-04740-z
_version_ 1760230833513299968
score 13.211869

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