Cover Image

Nursery evaluation of potential endophytic trichoderma spp. from North Sumatra, Indonesia as a biocontrol agent against ganoderma boninense

Basal stem rot (BSR) caused by the fungus Ganoderma boninense is regarded as the most destructive disease of oil palm (Elaeis guineensis Jaqc.) causes significant economic losses in the oil palm industry. An early study on the endophytic Trichoderma spp. showed they were promising potential in suppe...

Full description

Saved in:
Bibliographic Details
Main Authors: Dedek Haryadi, Hadi Hendra, Manjit Sidhu, Tumpal Panjaitan, Chong, Khim Phin
Format: Article
Language:English
Published: Indian Phytopathological Society 2021
Subjects:
QK710-899 Plant physiology
SB1-1110 Plant culture
Online Access:https://eprints.ums.edu.my/id/eprint/30866/1/Nursery%20evaluation%20of%20potential%20endophytic%20Trichoderma%20spp.%20from%20North%20Sumatra%2C%20Indonesia%20as%20a%20biocontrol%20agent%20against%20ganoderma%20boninense-ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/30866/
https://link.springer.com/article/10.1007/s42360-021-00348-w
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.ums.eprints.30866
record_format eprints
spelling my.ums.eprints.308662021-10-27T23:42:22Z https://eprints.ums.edu.my/id/eprint/30866/ Nursery evaluation of potential endophytic trichoderma spp. from North Sumatra, Indonesia as a biocontrol agent against ganoderma boninense Dedek Haryadi Hadi Hendra Manjit Sidhu Tumpal Panjaitan Chong, Khim Phin QK710-899 Plant physiology SB1-1110 Plant culture Basal stem rot (BSR) caused by the fungus Ganoderma boninense is regarded as the most destructive disease of oil palm (Elaeis guineensis Jaqc.) causes significant economic losses in the oil palm industry. An early study on the endophytic Trichoderma spp. showed they were promising potential in suppessing and preventing BSR. However, investigation on the effectiveness of endophytic Trichoderma from North Sumatra, Indonesia in preventing BSR disease in oil palms still scarce. The aim of this study was to evaluate the effectiveness of endophytic Trichoderma from North Sumatra, Indonesia in preventing BSR disease in the nursery. The active Trichoderma mixture (ATM) was prepared by first grown in PDA for six days before mixed and homogenized in 3 ml of sterile water. The mixture was later inoculated with a ratio of 1 ml to 150 g of mixture of cooked and sterilized broken corn and rice (3:4) medium. ATM was further dried and mixed homogeneously with zeolite (1:10) for application in the nursery. The potential endophytic Trichoderma spp. in suppressing BSR was monitored in the nursery for ten months. The external and internal symptoms of disease development were observed and disease incidence (DI) was also recorded. Total phenolic content (TPC) was analyzed using Folin-Ciocalteu method to determine the effect of endophytic Trichoderma in inducing oil palm seedling resistance to BSR disease. The result showed that application of endophytic Trichoderma spp. was found to be effective in suppressing G. boninense infection, where endophytic T. reesei ET501 to be the most effective in preventing disease incidence (DI) with only 13.4 % infection after ten months of observation followed by endophytic T. asperellum ET537 (48.4 %) and T. asperellum ET523 (71.8 %), respectively. Application of endophytic Trichoderma spp. in the nursery also exhibited the potential in inducing TPC amount and it helps in developing resistance in oil palm seedlings, thus protecting the seedlings from G. boninense infection. Indian Phytopathological Society 2021-03-28 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/30866/1/Nursery%20evaluation%20of%20potential%20endophytic%20Trichoderma%20spp.%20from%20North%20Sumatra%2C%20Indonesia%20as%20a%20biocontrol%20agent%20against%20ganoderma%20boninense-ABSTRACT.pdf Dedek Haryadi and Hadi Hendra and Manjit Sidhu and Tumpal Panjaitan and Chong, Khim Phin (2021) Nursery evaluation of potential endophytic trichoderma spp. from North Sumatra, Indonesia as a biocontrol agent against ganoderma boninense. Indian Phytopathology, 74. pp. 803-808. ISSN 0367-973X (P-ISSN) , 2248-9800 (E-ISSN) https://link.springer.com/article/10.1007/s42360-021-00348-w
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
topic QK710-899 Plant physiology
SB1-1110 Plant culture
spellingShingle QK710-899 Plant physiology
SB1-1110 Plant culture
Dedek Haryadi
Hadi Hendra
Manjit Sidhu
Tumpal Panjaitan
Chong, Khim Phin
Nursery evaluation of potential endophytic trichoderma spp. from North Sumatra, Indonesia as a biocontrol agent against ganoderma boninense
description Basal stem rot (BSR) caused by the fungus Ganoderma boninense is regarded as the most destructive disease of oil palm (Elaeis guineensis Jaqc.) causes significant economic losses in the oil palm industry. An early study on the endophytic Trichoderma spp. showed they were promising potential in suppessing and preventing BSR. However, investigation on the effectiveness of endophytic Trichoderma from North Sumatra, Indonesia in preventing BSR disease in oil palms still scarce. The aim of this study was to evaluate the effectiveness of endophytic Trichoderma from North Sumatra, Indonesia in preventing BSR disease in the nursery. The active Trichoderma mixture (ATM) was prepared by first grown in PDA for six days before mixed and homogenized in 3 ml of sterile water. The mixture was later inoculated with a ratio of 1 ml to 150 g of mixture of cooked and sterilized broken corn and rice (3:4) medium. ATM was further dried and mixed homogeneously with zeolite (1:10) for application in the nursery. The potential endophytic Trichoderma spp. in suppressing BSR was monitored in the nursery for ten months. The external and internal symptoms of disease development were observed and disease incidence (DI) was also recorded. Total phenolic content (TPC) was analyzed using Folin-Ciocalteu method to determine the effect of endophytic Trichoderma in inducing oil palm seedling resistance to BSR disease. The result showed that application of endophytic Trichoderma spp. was found to be effective in suppressing G. boninense infection, where endophytic T. reesei ET501 to be the most effective in preventing disease incidence (DI) with only 13.4 % infection after ten months of observation followed by endophytic T. asperellum ET537 (48.4 %) and T. asperellum ET523 (71.8 %), respectively. Application of endophytic Trichoderma spp. in the nursery also exhibited the potential in inducing TPC amount and it helps in developing resistance in oil palm seedlings, thus protecting the seedlings from G. boninense infection.
format Article
author Dedek Haryadi
Hadi Hendra
Manjit Sidhu
Tumpal Panjaitan
Chong, Khim Phin
author_facet Dedek Haryadi
Hadi Hendra
Manjit Sidhu
Tumpal Panjaitan
Chong, Khim Phin
author_sort Dedek Haryadi
title Nursery evaluation of potential endophytic trichoderma spp. from North Sumatra, Indonesia as a biocontrol agent against ganoderma boninense
title_short Nursery evaluation of potential endophytic trichoderma spp. from North Sumatra, Indonesia as a biocontrol agent against ganoderma boninense
title_full Nursery evaluation of potential endophytic trichoderma spp. from North Sumatra, Indonesia as a biocontrol agent against ganoderma boninense
title_fullStr Nursery evaluation of potential endophytic trichoderma spp. from North Sumatra, Indonesia as a biocontrol agent against ganoderma boninense
title_full_unstemmed Nursery evaluation of potential endophytic trichoderma spp. from North Sumatra, Indonesia as a biocontrol agent against ganoderma boninense
title_sort nursery evaluation of potential endophytic trichoderma spp. from north sumatra, indonesia as a biocontrol agent against ganoderma boninense
publisher Indian Phytopathological Society
publishDate 2021
url https://eprints.ums.edu.my/id/eprint/30866/1/Nursery%20evaluation%20of%20potential%20endophytic%20Trichoderma%20spp.%20from%20North%20Sumatra%2C%20Indonesia%20as%20a%20biocontrol%20agent%20against%20ganoderma%20boninense-ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/30866/
https://link.springer.com/article/10.1007/s42360-021-00348-w
_version_ 1760230818277490688
score 13.211869

Similar Items