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Optimisation of ultrasound assisted extraction of antiacetylcholinesterase and antioxidant compounds from manuka (Leptospermum scoparium) for use as a phytomedicine against alzheimer’s disease

Background: Alzheimer’s disease is a progressive mental deterioration related to ageing and senility. Approved drugs that inhibit acetylcholinesterase (AChE) enzyme activity in the human brain are one of the ways to control the natural progression of this disease. The present study reports on the op...

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Main Authors: Hafiz Majid, Filipa V. M. Silva
Format: Article
Language:English
English
Published: Scion Publishing 2020
Subjects:
QD Chemistry
RA Public aspects of medicine
Online Access:https://eprints.ums.edu.my/id/eprint/29963/1/Optimisation%20of%20ultrasound%20assisted%20extraction%20of%20antiacetylcholinesterase%20and%20antioxidant%20compounds%20from%20manuka%20%28Leptospermum%20scoparium%29%20for%20use%20as%20a%20phytomedicine%20against%20alzheimer%E2%80%99s%20disease%20abstract.pdf
https://eprints.ums.edu.my/id/eprint/29963/2/Optimisation%20of%20ultrasound%20assisted%20extraction%20of%20antiacetylcholinesterase%20and%20antioxidant%20compounds%20from%20manuka.pdf
https://eprints.ums.edu.my/id/eprint/29963/
https://nzjforestryscience.nz/index.php/nzjfs/article/view/103
https://doi.org/10.33494/nzjfs502020x103x
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spelling my.ums.eprints.299632021-07-27T14:11:52Z https://eprints.ums.edu.my/id/eprint/29963/ Optimisation of ultrasound assisted extraction of antiacetylcholinesterase and antioxidant compounds from manuka (Leptospermum scoparium) for use as a phytomedicine against alzheimer’s disease Hafiz Majid Filipa V. M. Silva QD Chemistry RA Public aspects of medicine Background: Alzheimer’s disease is a progressive mental deterioration related to ageing and senility. Approved drugs that inhibit acetylcholinesterase (AChE) enzyme activity in the human brain are one of the ways to control the natural progression of this disease. The present study reports on the optimisation of ultrasound-assisted extraction of antiacetylcholinesterase and antioxidant compounds from manuka leaves using response surface methodology. Methods: A Box-Behnken design was used to investigate the effect of extraction temperature (40–60°C), time (1–20 min), and ethanol concentration (30–70%) on AChE inhibition, antioxidant activity, and extraction yield. Results: The values of AChE, radical scavenging activity (RSA) and yield predicted by the models generated were similar to the experimental values. Extraction time, ethanol concentration and temperature were significant in all the responses. Optimum extraction conditions for maximum AChE inhibition (74%), RSA (79%) and yield (50%) were successfully validated experimentally and the IC50 of the optimised extracts were reduced to 28.5 (from 66.0) and 2.37 (from 32.4) μg/ mL for AChE and antioxidant activity, respectively. The optimisation enabled an increase in the extraction yield from 21% to 49%. Conclusions: In view of the significant bioactive properties determined, with possible beneficial effects on memory deficit, we would encourage the use of the manuka leaf extract for the development of new phytopharmaceuticals to improve brain function and control dementias such as Alzheimer’s disease. One other application could be as a beverage for the preparation of tea infusions. Scion Publishing 2020 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/29963/1/Optimisation%20of%20ultrasound%20assisted%20extraction%20of%20antiacetylcholinesterase%20and%20antioxidant%20compounds%20from%20manuka%20%28Leptospermum%20scoparium%29%20for%20use%20as%20a%20phytomedicine%20against%20alzheimer%E2%80%99s%20disease%20abstract.pdf text en https://eprints.ums.edu.my/id/eprint/29963/2/Optimisation%20of%20ultrasound%20assisted%20extraction%20of%20antiacetylcholinesterase%20and%20antioxidant%20compounds%20from%20manuka.pdf Hafiz Majid and Filipa V. M. Silva (2020) Optimisation of ultrasound assisted extraction of antiacetylcholinesterase and antioxidant compounds from manuka (Leptospermum scoparium) for use as a phytomedicine against alzheimer’s disease. New Zealand Journal of Forestry Science, 50. pp. 1-12. ISSN 0048-0134 (P-ISSN) , 1179-5395 (E-ISSN) (Submitted) https://nzjforestryscience.nz/index.php/nzjfs/article/view/103 https://doi.org/10.33494/nzjfs502020x103x
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
English
topic QD Chemistry
RA Public aspects of medicine
spellingShingle QD Chemistry
RA Public aspects of medicine
Hafiz Majid
Filipa V. M. Silva
Optimisation of ultrasound assisted extraction of antiacetylcholinesterase and antioxidant compounds from manuka (Leptospermum scoparium) for use as a phytomedicine against alzheimer’s disease
description Background: Alzheimer’s disease is a progressive mental deterioration related to ageing and senility. Approved drugs that inhibit acetylcholinesterase (AChE) enzyme activity in the human brain are one of the ways to control the natural progression of this disease. The present study reports on the optimisation of ultrasound-assisted extraction of antiacetylcholinesterase and antioxidant compounds from manuka leaves using response surface methodology. Methods: A Box-Behnken design was used to investigate the effect of extraction temperature (40–60°C), time (1–20 min), and ethanol concentration (30–70%) on AChE inhibition, antioxidant activity, and extraction yield. Results: The values of AChE, radical scavenging activity (RSA) and yield predicted by the models generated were similar to the experimental values. Extraction time, ethanol concentration and temperature were significant in all the responses. Optimum extraction conditions for maximum AChE inhibition (74%), RSA (79%) and yield (50%) were successfully validated experimentally and the IC50 of the optimised extracts were reduced to 28.5 (from 66.0) and 2.37 (from 32.4) μg/ mL for AChE and antioxidant activity, respectively. The optimisation enabled an increase in the extraction yield from 21% to 49%. Conclusions: In view of the significant bioactive properties determined, with possible beneficial effects on memory deficit, we would encourage the use of the manuka leaf extract for the development of new phytopharmaceuticals to improve brain function and control dementias such as Alzheimer’s disease. One other application could be as a beverage for the preparation of tea infusions.
format Article
author Hafiz Majid
Filipa V. M. Silva
author_facet Hafiz Majid
Filipa V. M. Silva
author_sort Hafiz Majid
title Optimisation of ultrasound assisted extraction of antiacetylcholinesterase and antioxidant compounds from manuka (Leptospermum scoparium) for use as a phytomedicine against alzheimer’s disease
title_short Optimisation of ultrasound assisted extraction of antiacetylcholinesterase and antioxidant compounds from manuka (Leptospermum scoparium) for use as a phytomedicine against alzheimer’s disease
title_full Optimisation of ultrasound assisted extraction of antiacetylcholinesterase and antioxidant compounds from manuka (Leptospermum scoparium) for use as a phytomedicine against alzheimer’s disease
title_fullStr Optimisation of ultrasound assisted extraction of antiacetylcholinesterase and antioxidant compounds from manuka (Leptospermum scoparium) for use as a phytomedicine against alzheimer’s disease
title_full_unstemmed Optimisation of ultrasound assisted extraction of antiacetylcholinesterase and antioxidant compounds from manuka (Leptospermum scoparium) for use as a phytomedicine against alzheimer’s disease
title_sort optimisation of ultrasound assisted extraction of antiacetylcholinesterase and antioxidant compounds from manuka (leptospermum scoparium) for use as a phytomedicine against alzheimer’s disease
publisher Scion Publishing
publishDate 2020
url https://eprints.ums.edu.my/id/eprint/29963/1/Optimisation%20of%20ultrasound%20assisted%20extraction%20of%20antiacetylcholinesterase%20and%20antioxidant%20compounds%20from%20manuka%20%28Leptospermum%20scoparium%29%20for%20use%20as%20a%20phytomedicine%20against%20alzheimer%E2%80%99s%20disease%20abstract.pdf
https://eprints.ums.edu.my/id/eprint/29963/2/Optimisation%20of%20ultrasound%20assisted%20extraction%20of%20antiacetylcholinesterase%20and%20antioxidant%20compounds%20from%20manuka.pdf
https://eprints.ums.edu.my/id/eprint/29963/
https://nzjforestryscience.nz/index.php/nzjfs/article/view/103
https://doi.org/10.33494/nzjfs502020x103x
_version_ 1760230701359169536
score 13.211869

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