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Selecting antagonistic yeast for postharvest biocontrol of Colletotrichum gloeosporioides in papaya fruit and possible mechanisms involved

Colletotrichum gloeosporioides causes anthracnose disease in papaya fruit resulting in tremendous economic loss due to its latent infection. This study aimed to evaluate the biocontrol activity of antagonistic yeasts against C. gloeosporioides in papaya and determine the possible mechanism involved....

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Main Authors: Hamizah Hassan, Mahmud Tengku Muda Mohamed, Siti Fairuz Yusoff, Erneeza Mohd Hata, Nor Elliza Tajidin
Format: Article
Language:English
English
Published: MDPI AG 2021
Subjects:
S Agriculture (General)
Online Access:https://eprints.ums.edu.my/id/eprint/29927/1/Selecting%20antagonistic%20yeast%20for%20postharvest%20biocontrol%20of%20Colletotrichum%20gloeosporioides%20in%20papaya%20fruit%20and%20possible%20mechanisms%20involved%20FULL%20TEXT.pdf
https://eprints.ums.edu.my/id/eprint/29927/2/Selecting%20antagonistic%20yeast%20for%20postharvest%20biocontrol%20of%20Colletotrichum%20gloeosporioides%20in%20papaya%20fruit%20and%20possible%20mechanisms%20involved%20ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/29927/
https://www.mdpi.com/2073-4395/11/4/760/htm
https://doi.org/10.3390/agronomy11040760
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spelling my.ums.eprints.299272021-07-14T00:42:24Z https://eprints.ums.edu.my/id/eprint/29927/ Selecting antagonistic yeast for postharvest biocontrol of Colletotrichum gloeosporioides in papaya fruit and possible mechanisms involved Hamizah Hassan Mahmud Tengku Muda Mohamed Siti Fairuz Yusoff Erneeza Mohd Hata Nor Elliza Tajidin S Agriculture (General) Colletotrichum gloeosporioides causes anthracnose disease in papaya fruit resulting in tremendous economic loss due to its latent infection. This study aimed to evaluate the biocontrol activity of antagonistic yeasts against C. gloeosporioides in papaya and determine the possible mechanism involved. One hundred and ten yeast strains were isolated from different parts of the papaya plant. Among them, only five strains, namely F001, F006, L003, FL013 and LP010, showed more than 55% radial growth inhibition of C. gloeosporioides. These five potent yeast strains were further evaluated in vitro and in vivo. The results indicated that strain F001 had the strongest biocontrol activity based on spore germination and fungal growth inhibition. In Vivo, the strain F001 caused 66.7% and 25% reductions in disease incidence and severity, respectively. Based on molecular identification, the strain F001 was confirmed as Trichosporon asahii. Despite there was no significant induction of defense enzyme activities found on the treated fruits, SEM observation showed direct attachment of T. asahii with the fungal hyphae and interfere in their establishment to the fruit surface. Based on these findings, the antagonistic yeast T. asahii strain F001 may be used as a potential natural biological control agent against anthracnose disease in papaya fruit MDPI AG 2021 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/29927/1/Selecting%20antagonistic%20yeast%20for%20postharvest%20biocontrol%20of%20Colletotrichum%20gloeosporioides%20in%20papaya%20fruit%20and%20possible%20mechanisms%20involved%20FULL%20TEXT.pdf text en https://eprints.ums.edu.my/id/eprint/29927/2/Selecting%20antagonistic%20yeast%20for%20postharvest%20biocontrol%20of%20Colletotrichum%20gloeosporioides%20in%20papaya%20fruit%20and%20possible%20mechanisms%20involved%20ABSTRACT.pdf Hamizah Hassan and Mahmud Tengku Muda Mohamed and Siti Fairuz Yusoff and Erneeza Mohd Hata and Nor Elliza Tajidin (2021) Selecting antagonistic yeast for postharvest biocontrol of Colletotrichum gloeosporioides in papaya fruit and possible mechanisms involved. Agronomy, 11 (4). pp. 1-17. ISSN 2073-4395 https://www.mdpi.com/2073-4395/11/4/760/htm https://doi.org/10.3390/agronomy11040760
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
English
topic S Agriculture (General)
spellingShingle S Agriculture (General)
Hamizah Hassan
Mahmud Tengku Muda Mohamed
Siti Fairuz Yusoff
Erneeza Mohd Hata
Nor Elliza Tajidin
Selecting antagonistic yeast for postharvest biocontrol of Colletotrichum gloeosporioides in papaya fruit and possible mechanisms involved
description Colletotrichum gloeosporioides causes anthracnose disease in papaya fruit resulting in tremendous economic loss due to its latent infection. This study aimed to evaluate the biocontrol activity of antagonistic yeasts against C. gloeosporioides in papaya and determine the possible mechanism involved. One hundred and ten yeast strains were isolated from different parts of the papaya plant. Among them, only five strains, namely F001, F006, L003, FL013 and LP010, showed more than 55% radial growth inhibition of C. gloeosporioides. These five potent yeast strains were further evaluated in vitro and in vivo. The results indicated that strain F001 had the strongest biocontrol activity based on spore germination and fungal growth inhibition. In Vivo, the strain F001 caused 66.7% and 25% reductions in disease incidence and severity, respectively. Based on molecular identification, the strain F001 was confirmed as Trichosporon asahii. Despite there was no significant induction of defense enzyme activities found on the treated fruits, SEM observation showed direct attachment of T. asahii with the fungal hyphae and interfere in their establishment to the fruit surface. Based on these findings, the antagonistic yeast T. asahii strain F001 may be used as a potential natural biological control agent against anthracnose disease in papaya fruit
format Article
author Hamizah Hassan
Mahmud Tengku Muda Mohamed
Siti Fairuz Yusoff
Erneeza Mohd Hata
Nor Elliza Tajidin
author_facet Hamizah Hassan
Mahmud Tengku Muda Mohamed
Siti Fairuz Yusoff
Erneeza Mohd Hata
Nor Elliza Tajidin
author_sort Hamizah Hassan
title Selecting antagonistic yeast for postharvest biocontrol of Colletotrichum gloeosporioides in papaya fruit and possible mechanisms involved
title_short Selecting antagonistic yeast for postharvest biocontrol of Colletotrichum gloeosporioides in papaya fruit and possible mechanisms involved
title_full Selecting antagonistic yeast for postharvest biocontrol of Colletotrichum gloeosporioides in papaya fruit and possible mechanisms involved
title_fullStr Selecting antagonistic yeast for postharvest biocontrol of Colletotrichum gloeosporioides in papaya fruit and possible mechanisms involved
title_full_unstemmed Selecting antagonistic yeast for postharvest biocontrol of Colletotrichum gloeosporioides in papaya fruit and possible mechanisms involved
title_sort selecting antagonistic yeast for postharvest biocontrol of colletotrichum gloeosporioides in papaya fruit and possible mechanisms involved
publisher MDPI AG
publishDate 2021
url https://eprints.ums.edu.my/id/eprint/29927/1/Selecting%20antagonistic%20yeast%20for%20postharvest%20biocontrol%20of%20Colletotrichum%20gloeosporioides%20in%20papaya%20fruit%20and%20possible%20mechanisms%20involved%20FULL%20TEXT.pdf
https://eprints.ums.edu.my/id/eprint/29927/2/Selecting%20antagonistic%20yeast%20for%20postharvest%20biocontrol%20of%20Colletotrichum%20gloeosporioides%20in%20papaya%20fruit%20and%20possible%20mechanisms%20involved%20ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/29927/
https://www.mdpi.com/2073-4395/11/4/760/htm
https://doi.org/10.3390/agronomy11040760
_version_ 1760230696558788608
score 13.211869

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