Staff View: An alternative inhibitive assay using Monopterus albus (Asian swamp eel) acetylcholinesterase for metal ions detection

An alternative inhibitive assay using Monopterus albus (Asian swamp eel) acetylcholinesterase for metal ions detection

Introduction: Cholinesterase-based biosensor well known as a sensitive method to detect the existence of harmful dissolve compound in any types of water sources especially river. Methods: This method can be applied as a preliminary screening to determine the contamination level of the river in a sho...

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Main Authors:	Siti Aishah Muhammad Khalidi, Mohd Khalizan Sabullah, Suraya Abd. Sani, Mohd Yunus Shukor, Siti Aqlima Ahmad, Nur Adeela Yasid, ‘Izazy Nur Mohd Jaafar, Gunasekaran Baskaran
Format:	Conference or Workshop Item
Language:	English English
Published:	Universiti Putra Malaysia 2019
Subjects:	SH171-179 Diseases and adverse factors
Online Access:	https://eprints.ums.edu.my/id/eprint/29034/1/An%20alternative%20inhibitive%20assay%20using%20Monopterus%20albus%20%28Asian%20swamp%20eel%29%20acetylcholinesterase%20for%20metal%20ions%20detection%20ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/29034/3/An%20alternative%20inhibitive%20assay%20using%20Monopterus%20albus%20%28Asian%20swamp%20eel%29%20acetylcholinesterase%20for%20metal%20ions%20detection.pdf https://eprints.ums.edu.my/id/eprint/29034/ http://www.msmbb.my/images/publication/volume_27/issue_2/Complete-APJMBB272-supplementary_20190618.pdf
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spelling	my.ums.eprints.290342021-09-20T00:59:17Z https://eprints.ums.edu.my/id/eprint/29034/ An alternative inhibitive assay using Monopterus albus (Asian swamp eel) acetylcholinesterase for metal ions detection Siti Aishah Muhammad Khalidi Mohd Khalizan Sabullah Suraya Abd. Sani Mohd Yunus Shukor Siti Aqlima Ahmad Nur Adeela Yasid ‘Izazy Nur Mohd Jaafar Gunasekaran Baskaran SH171-179 Diseases and adverse factors Introduction: Cholinesterase-based biosensor well known as a sensitive method to detect the existence of harmful dissolve compound in any types of water sources especially river. Methods: This method can be applied as a preliminary screening to determine the contamination level of the river in a short of time as well as low cost and easy to operate. The aim of this study to assess the use of acetylcholinesterase source from the brain tissue of Asian swamp eel; Monopterus albus as a potential environmental biosensor. Results: Purified acetylcholinesterase was exposed with a different type of metal ion, and mercury show the highest inhibition which capable of lowering the enzyme activity to 0.018U followed by copper (1.280U), chromium (4.675U) and zinc (4.803U) at 10 ppm. Metal ions show exponential decay type inhibition curves with calculated half maximal inhibitory concentration; IC50 in the ascending sensitivity order of copper, chromium, zinc and mercury at 1.329, 0.687, 0.595 and 0.004 ppm, respectively. Field trial works exhibited that the enzyme was applicable in sensing heavy metals pollution from the river which closes to the industrial and agricultural sites at near real time and verified using ICP-OES. Conclusion: This study proves the potential use of acetylcholinesterase source from M. albus as a biomonitoring tool to evaluate the contamination level of the river. Universiti Putra Malaysia 2019-04-25 Conference or Workshop Item PeerReviewed text en https://eprints.ums.edu.my/id/eprint/29034/1/An%20alternative%20inhibitive%20assay%20using%20Monopterus%20albus%20%28Asian%20swamp%20eel%29%20acetylcholinesterase%20for%20metal%20ions%20detection%20ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/29034/3/An%20alternative%20inhibitive%20assay%20using%20Monopterus%20albus%20%28Asian%20swamp%20eel%29%20acetylcholinesterase%20for%20metal%20ions%20detection.pdf Siti Aishah Muhammad Khalidi and Mohd Khalizan Sabullah and Suraya Abd. Sani and Mohd Yunus Shukor and Siti Aqlima Ahmad and Nur Adeela Yasid and ‘Izazy Nur Mohd Jaafar and Gunasekaran Baskaran (2019) An alternative inhibitive assay using Monopterus albus (Asian swamp eel) acetylcholinesterase for metal ions detection. In: 3 rd International Conference on Molecular Biology and Biotechnology 2019, 24-25 April 2019, UCSI University Kuala Lumpur Campus. http://www.msmbb.my/images/publication/volume_27/issue_2/Complete-APJMBB272-supplementary_20190618.pdf
institution	Universiti Malaysia Sabah
building	UMS Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaysia Sabah
content_source	UMS Institutional Repository
url_provider	http://eprints.ums.edu.my/
language	English English
topic	SH171-179 Diseases and adverse factors
spellingShingle	SH171-179 Diseases and adverse factors Siti Aishah Muhammad Khalidi Mohd Khalizan Sabullah Suraya Abd. Sani Mohd Yunus Shukor Siti Aqlima Ahmad Nur Adeela Yasid ‘Izazy Nur Mohd Jaafar Gunasekaran Baskaran An alternative inhibitive assay using Monopterus albus (Asian swamp eel) acetylcholinesterase for metal ions detection
description	Introduction: Cholinesterase-based biosensor well known as a sensitive method to detect the existence of harmful dissolve compound in any types of water sources especially river. Methods: This method can be applied as a preliminary screening to determine the contamination level of the river in a short of time as well as low cost and easy to operate. The aim of this study to assess the use of acetylcholinesterase source from the brain tissue of Asian swamp eel; Monopterus albus as a potential environmental biosensor. Results: Purified acetylcholinesterase was exposed with a different type of metal ion, and mercury show the highest inhibition which capable of lowering the enzyme activity to 0.018U followed by copper (1.280U), chromium (4.675U) and zinc (4.803U) at 10 ppm. Metal ions show exponential decay type inhibition curves with calculated half maximal inhibitory concentration; IC50 in the ascending sensitivity order of copper, chromium, zinc and mercury at 1.329, 0.687, 0.595 and 0.004 ppm, respectively. Field trial works exhibited that the enzyme was applicable in sensing heavy metals pollution from the river which closes to the industrial and agricultural sites at near real time and verified using ICP-OES. Conclusion: This study proves the potential use of acetylcholinesterase source from M. albus as a biomonitoring tool to evaluate the contamination level of the river.
format	Conference or Workshop Item
author	Siti Aishah Muhammad Khalidi Mohd Khalizan Sabullah Suraya Abd. Sani Mohd Yunus Shukor Siti Aqlima Ahmad Nur Adeela Yasid ‘Izazy Nur Mohd Jaafar Gunasekaran Baskaran
author_facet	Siti Aishah Muhammad Khalidi Mohd Khalizan Sabullah Suraya Abd. Sani Mohd Yunus Shukor Siti Aqlima Ahmad Nur Adeela Yasid ‘Izazy Nur Mohd Jaafar Gunasekaran Baskaran
author_sort	Siti Aishah Muhammad Khalidi
title	An alternative inhibitive assay using Monopterus albus (Asian swamp eel) acetylcholinesterase for metal ions detection
title_short	An alternative inhibitive assay using Monopterus albus (Asian swamp eel) acetylcholinesterase for metal ions detection
title_full	An alternative inhibitive assay using Monopterus albus (Asian swamp eel) acetylcholinesterase for metal ions detection
title_fullStr	An alternative inhibitive assay using Monopterus albus (Asian swamp eel) acetylcholinesterase for metal ions detection
title_full_unstemmed	An alternative inhibitive assay using Monopterus albus (Asian swamp eel) acetylcholinesterase for metal ions detection
title_sort	alternative inhibitive assay using monopterus albus (asian swamp eel) acetylcholinesterase for metal ions detection
publisher	Universiti Putra Malaysia
publishDate	2019
url	https://eprints.ums.edu.my/id/eprint/29034/1/An%20alternative%20inhibitive%20assay%20using%20Monopterus%20albus%20%28Asian%20swamp%20eel%29%20acetylcholinesterase%20for%20metal%20ions%20detection%20ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/29034/3/An%20alternative%20inhibitive%20assay%20using%20Monopterus%20albus%20%28Asian%20swamp%20eel%29%20acetylcholinesterase%20for%20metal%20ions%20detection.pdf https://eprints.ums.edu.my/id/eprint/29034/ http://www.msmbb.my/images/publication/volume_27/issue_2/Complete-APJMBB272-supplementary_20190618.pdf
_version_	1760230664100118528
score	13.2442

An alternative inhibitive assay using Monopterus albus (Asian swamp eel) acetylcholinesterase for metal ions detection

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