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Assessment of inhibitive assay for insecticides using acetylcholinesterase from Puntius schwanenfeldii

In this study, the substrate specificity and the inhibition kinetics of various types of insecticides to the acetylcholinesterase (AChE) from a local fish; Puntius schwanenfeldii were investigated. The substrate specificity determination was done using three thiocholine substrates,...

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Main Authors: Mohd Khalizan Sabullah, Mohd Ezuan Khayat
Format: Article
Language:English
Published: Hibiscus Publisher 2015
Subjects:
QD Chemistry
QH301-705.5 Biology (General)
SH Aquaculture. Fisheries. Angling
Online Access:https://eprints.ums.edu.my/id/eprint/27477/1/Assessment%20of%20inhibitive%20assay%20for%20insecticides%20using%20acetylcholinesterase%20from%20Puntius%20schwanenfeldii%20FULL%20TEXT.pdf
https://eprints.ums.edu.my/id/eprint/27477/
https://journal.hibiscuspublisher.com/index.php/JOBIMB/article/view/278/316
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spelling my.ums.eprints.274772021-06-28T23:37:09Z https://eprints.ums.edu.my/id/eprint/27477/ Assessment of inhibitive assay for insecticides using acetylcholinesterase from Puntius schwanenfeldii Mohd Khalizan Sabullah Mohd Ezuan Khayat QD Chemistry QH301-705.5 Biology (General) SH Aquaculture. Fisheries. Angling In this study, the substrate specificity and the inhibition kinetics of various types of insecticides to the acetylcholinesterase (AChE) from a local fish; Puntius schwanenfeldii were investigated. The substrate specificity determination was done using three thiocholine substrates, which were ATC, PTC and BTC. The results showed that he partially purified cholinesterase from Puntius schwanenfeldii that preferred ATC is a true AChE. The Km and Vmax values of AChE for these substrates were 16.61 mmol and 286.5 U/mg for ATC, 19.92 mmol and 245.3 U/mg for PTC, and 48.64 mmol and 219.6 U/mg for BTC, respectively. The IC50 values for the carbamates bendiocarb, carbaryl, propoxur, carbofuran and methomyl were 0.838, 7.045, 29.441, 1.411 and 8.335 mg/L, respectively, which were comparable to the IC50 values for carbamates from several AChE from fish. Hibiscus Publisher 2015 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/27477/1/Assessment%20of%20inhibitive%20assay%20for%20insecticides%20using%20acetylcholinesterase%20from%20Puntius%20schwanenfeldii%20FULL%20TEXT.pdf Mohd Khalizan Sabullah and Mohd Ezuan Khayat (2015) Assessment of inhibitive assay for insecticides using acetylcholinesterase from Puntius schwanenfeldii. Journal of Biochemistry, Microbiology and Biotechnology, 3 (2). pp. 26-29. ISSN 2289-5779 https://journal.hibiscuspublisher.com/index.php/JOBIMB/article/view/278/316
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
topic QD Chemistry
QH301-705.5 Biology (General)
SH Aquaculture. Fisheries. Angling
spellingShingle QD Chemistry
QH301-705.5 Biology (General)
SH Aquaculture. Fisheries. Angling
Mohd Khalizan Sabullah
Mohd Ezuan Khayat
Assessment of inhibitive assay for insecticides using acetylcholinesterase from Puntius schwanenfeldii
description In this study, the substrate specificity and the inhibition kinetics of various types of insecticides to the acetylcholinesterase (AChE) from a local fish; Puntius schwanenfeldii were investigated. The substrate specificity determination was done using three thiocholine substrates, which were ATC, PTC and BTC. The results showed that he partially purified cholinesterase from Puntius schwanenfeldii that preferred ATC is a true AChE. The Km and Vmax values of AChE for these substrates were 16.61 mmol and 286.5 U/mg for ATC, 19.92 mmol and 245.3 U/mg for PTC, and 48.64 mmol and 219.6 U/mg for BTC, respectively. The IC50 values for the carbamates bendiocarb, carbaryl, propoxur, carbofuran and methomyl were 0.838, 7.045, 29.441, 1.411 and 8.335 mg/L, respectively, which were comparable to the IC50 values for carbamates from several AChE from fish.
format Article
author Mohd Khalizan Sabullah
Mohd Ezuan Khayat
author_facet Mohd Khalizan Sabullah
Mohd Ezuan Khayat
author_sort Mohd Khalizan Sabullah
title Assessment of inhibitive assay for insecticides using acetylcholinesterase from Puntius schwanenfeldii
title_short Assessment of inhibitive assay for insecticides using acetylcholinesterase from Puntius schwanenfeldii
title_full Assessment of inhibitive assay for insecticides using acetylcholinesterase from Puntius schwanenfeldii
title_fullStr Assessment of inhibitive assay for insecticides using acetylcholinesterase from Puntius schwanenfeldii
title_full_unstemmed Assessment of inhibitive assay for insecticides using acetylcholinesterase from Puntius schwanenfeldii
title_sort assessment of inhibitive assay for insecticides using acetylcholinesterase from puntius schwanenfeldii
publisher Hibiscus Publisher
publishDate 2015
url https://eprints.ums.edu.my/id/eprint/27477/1/Assessment%20of%20inhibitive%20assay%20for%20insecticides%20using%20acetylcholinesterase%20from%20Puntius%20schwanenfeldii%20FULL%20TEXT.pdf
https://eprints.ums.edu.my/id/eprint/27477/
https://journal.hibiscuspublisher.com/index.php/JOBIMB/article/view/278/316
_version_ 1760230626804367360
score 13.251813

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