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Leech blood-meal invertebrate-derived DNA reveals differences in Bornean mammal diversity across habitats

The application of metabarcoding to environmental and invertebrate-derived DNA (eDNA and iDNA) is a new and increasingly applied method for monitoring biodiversity across a diverse range of habitats. This approach is particularly promising for sampling in the biodiverse humid tropics, where rapid la...

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Main Authors: Drinkwater, R., Jucker, T., Potter, J.H.T., Swinfield, T., Coomes, D.A., Slade, E.M., Gilbert, M.T.P., Lewis, O.T., Henry Bernard, Struebig, M.J., Clare, E.L., Rossiter, S.J.
Format: Article
Language:English
Published: Blackwell Publishing Ltd 2020
Subjects:
Q Science (General)
QL Zoology
Online Access:https://eprints.ums.edu.my/id/eprint/26908/1/Leech%20blood-meal%20invertebrate-derived%20DNA%20reveals%20differences%20in%20Bornean%20mammal%20diversity%20across%20habitats.pdf
https://eprints.ums.edu.my/id/eprint/26908/
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spelling my.ums.eprints.269082021-05-04T02:12:03Z https://eprints.ums.edu.my/id/eprint/26908/ Leech blood-meal invertebrate-derived DNA reveals differences in Bornean mammal diversity across habitats Drinkwater, R. Jucker, T. Potter, J.H.T. Swinfield, T. Coomes, D.A. Slade, E.M. Gilbert, M.T.P. Lewis, O.T. Henry Bernard Struebig, M.J. Clare, E.L. Rossiter, S.J. Q Science (General) QL Zoology The application of metabarcoding to environmental and invertebrate-derived DNA (eDNA and iDNA) is a new and increasingly applied method for monitoring biodiversity across a diverse range of habitats. This approach is particularly promising for sampling in the biodiverse humid tropics, where rapid land-use change for agriculture means there is a growing need to understand the conservation value of the remaining mosaic and degraded landscapes. Here we use iDNA from blood-feeding leeches (Haemadipsa picta) to assess differences in mammalian diversity across a gradient of forest degradation in Sabah, Malaysian Borneo. We screened 557 individual leeches for mammal DNA by targeting fragments of the 16S rRNA gene and detected 14 mammalian genera. We recorded lower mammal diversity in the most heavily degraded forest compared to higher quality twice logged forest. Although the accumulation curves of diversity estimates were comparable across these habitat types, diversity was higher in twice logged forest, with more taxa of conservation concern. In addition, our analysis revealed differences between the community recorded in the heavily logged forest and that of the twice logged forest. By revealing differences in mammal diversity across a human-modified tropical landscape, our study demonstrates the value of iDNA as a noninvasive biomonitoring approach in conservation assessments. © 2020 The Authors. Molecular Ecology published by John Wiley & Sons Ltd Blackwell Publishing Ltd 2020 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/26908/1/Leech%20blood-meal%20invertebrate-derived%20DNA%20reveals%20differences%20in%20Bornean%20mammal%20diversity%20across%20habitats.pdf Drinkwater, R. and Jucker, T. and Potter, J.H.T. and Swinfield, T. and Coomes, D.A. and Slade, E.M. and Gilbert, M.T.P. and Lewis, O.T. and Henry Bernard and Struebig, M.J. and Clare, E.L. and Rossiter, S.J. (2020) Leech blood-meal invertebrate-derived DNA reveals differences in Bornean mammal diversity across habitats. Molecular Ecology. ISSN 0962-1083 https://www.scopus.com/record/display.uri?eid=2-s2.0-85096862764&origin=inward&txGid=622613acaaa856bf2d0439a6cdb39392
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
topic Q Science (General)
QL Zoology
spellingShingle Q Science (General)
QL Zoology
Drinkwater, R.
Jucker, T.
Potter, J.H.T.
Swinfield, T.
Coomes, D.A.
Slade, E.M.
Gilbert, M.T.P.
Lewis, O.T.
Henry Bernard
Struebig, M.J.
Clare, E.L.
Rossiter, S.J.
Leech blood-meal invertebrate-derived DNA reveals differences in Bornean mammal diversity across habitats
description The application of metabarcoding to environmental and invertebrate-derived DNA (eDNA and iDNA) is a new and increasingly applied method for monitoring biodiversity across a diverse range of habitats. This approach is particularly promising for sampling in the biodiverse humid tropics, where rapid land-use change for agriculture means there is a growing need to understand the conservation value of the remaining mosaic and degraded landscapes. Here we use iDNA from blood-feeding leeches (Haemadipsa picta) to assess differences in mammalian diversity across a gradient of forest degradation in Sabah, Malaysian Borneo. We screened 557 individual leeches for mammal DNA by targeting fragments of the 16S rRNA gene and detected 14 mammalian genera. We recorded lower mammal diversity in the most heavily degraded forest compared to higher quality twice logged forest. Although the accumulation curves of diversity estimates were comparable across these habitat types, diversity was higher in twice logged forest, with more taxa of conservation concern. In addition, our analysis revealed differences between the community recorded in the heavily logged forest and that of the twice logged forest. By revealing differences in mammal diversity across a human-modified tropical landscape, our study demonstrates the value of iDNA as a noninvasive biomonitoring approach in conservation assessments. © 2020 The Authors. Molecular Ecology published by John Wiley & Sons Ltd
format Article
author Drinkwater, R.
Jucker, T.
Potter, J.H.T.
Swinfield, T.
Coomes, D.A.
Slade, E.M.
Gilbert, M.T.P.
Lewis, O.T.
Henry Bernard
Struebig, M.J.
Clare, E.L.
Rossiter, S.J.
author_facet Drinkwater, R.
Jucker, T.
Potter, J.H.T.
Swinfield, T.
Coomes, D.A.
Slade, E.M.
Gilbert, M.T.P.
Lewis, O.T.
Henry Bernard
Struebig, M.J.
Clare, E.L.
Rossiter, S.J.
author_sort Drinkwater, R.
title Leech blood-meal invertebrate-derived DNA reveals differences in Bornean mammal diversity across habitats
title_short Leech blood-meal invertebrate-derived DNA reveals differences in Bornean mammal diversity across habitats
title_full Leech blood-meal invertebrate-derived DNA reveals differences in Bornean mammal diversity across habitats
title_fullStr Leech blood-meal invertebrate-derived DNA reveals differences in Bornean mammal diversity across habitats
title_full_unstemmed Leech blood-meal invertebrate-derived DNA reveals differences in Bornean mammal diversity across habitats
title_sort leech blood-meal invertebrate-derived dna reveals differences in bornean mammal diversity across habitats
publisher Blackwell Publishing Ltd
publishDate 2020
url https://eprints.ums.edu.my/id/eprint/26908/1/Leech%20blood-meal%20invertebrate-derived%20DNA%20reveals%20differences%20in%20Bornean%20mammal%20diversity%20across%20habitats.pdf
https://eprints.ums.edu.my/id/eprint/26908/
https://www.scopus.com/record/display.uri?eid=2-s2.0-85096862764&origin=inward&txGid=622613acaaa856bf2d0439a6cdb39392
_version_ 1760230559333744640
score 13.222552

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