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Arabidopsis REI-LIKE proteins activate ribosome biogenesis during cold acclimation

Arabidopsis REIL proteins are cytosolic ribosomal 60S-biogenesis factors. After shift to 10 °C, reil mutants deplete and slowly replenish non-translating eukaryotic ribosome complexes of root tissue, while controlling the balance of non-translating 40S- and 60S-subunits. Reil mutations respond by hy...

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Main Authors: Cheong Bo Eng, Beine-Golovchuk, O., Gorka, M., Ho, W.W.H., Martinez-Seidel, F., Firmino, A.A.P., Skirycz, A., Roessner, U., Kopka, J.
Format: Article
Language:English
Published: Nature Research 2021
Subjects:
Q Science (General)
QK Botany
Online Access:https://eprints.ums.edu.my/id/eprint/26872/1/Arabidopsis%20REI-LIKE%20proteins%20activate%20ribosome%20biogenesis%20during%20cold%20acclimation.pdf
https://eprints.ums.edu.my/id/eprint/26872/
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spelling my.ums.eprints.268722021-04-30T02:21:14Z https://eprints.ums.edu.my/id/eprint/26872/ Arabidopsis REI-LIKE proteins activate ribosome biogenesis during cold acclimation Cheong Bo Eng Beine-Golovchuk, O. Gorka, M. Ho, W.W.H. Martinez-Seidel, F. Firmino, A.A.P. Skirycz, A. Roessner, U. Kopka, J. Q Science (General) QK Botany Arabidopsis REIL proteins are cytosolic ribosomal 60S-biogenesis factors. After shift to 10 °C, reil mutants deplete and slowly replenish non-translating eukaryotic ribosome complexes of root tissue, while controlling the balance of non-translating 40S- and 60S-subunits. Reil mutations respond by hyper-accumulation of non-translating subunits at steady-state temperature; after cold-shift, a KCl-sensitive 80S sub-fraction remains depleted. We infer that Arabidopsis may buffer fluctuating translation by pre-existing non-translating ribosomes before de novo synthesis meets temperature-induced demands. Reil1 reil2 double mutants accumulate 43S-preinitiation and pre-60S-maturation complexes and alter paralog composition of ribosomal proteins in non-translating complexes. With few exceptions, e.g. RPL3B and RPL24C, these changes are not under transcriptional control. Our study suggests requirement of de novo synthesis of eukaryotic ribosomes for long-term cold acclimation, feedback control of NUC2 and eIF3C2 transcription and links new proteins, AT1G03250, AT5G60530, to plant ribosome biogenesis. We propose that Arabidopsis requires biosynthesis of specialized ribosomes for cold acclimation. Nature Research 2021 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/26872/1/Arabidopsis%20REI-LIKE%20proteins%20activate%20ribosome%20biogenesis%20during%20cold%20acclimation.pdf Cheong Bo Eng and Beine-Golovchuk, O. and Gorka, M. and Ho, W.W.H. and Martinez-Seidel, F. and Firmino, A.A.P. and Skirycz, A. and Roessner, U. and Kopka, J. (2021) Arabidopsis REI-LIKE proteins activate ribosome biogenesis during cold acclimation. Scientific Reports, 11 (1). ISSN 2045-2322
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
topic Q Science (General)
QK Botany
spellingShingle Q Science (General)
QK Botany
Cheong Bo Eng
Beine-Golovchuk, O.
Gorka, M.
Ho, W.W.H.
Martinez-Seidel, F.
Firmino, A.A.P.
Skirycz, A.
Roessner, U.
Kopka, J.
Arabidopsis REI-LIKE proteins activate ribosome biogenesis during cold acclimation
description Arabidopsis REIL proteins are cytosolic ribosomal 60S-biogenesis factors. After shift to 10 °C, reil mutants deplete and slowly replenish non-translating eukaryotic ribosome complexes of root tissue, while controlling the balance of non-translating 40S- and 60S-subunits. Reil mutations respond by hyper-accumulation of non-translating subunits at steady-state temperature; after cold-shift, a KCl-sensitive 80S sub-fraction remains depleted. We infer that Arabidopsis may buffer fluctuating translation by pre-existing non-translating ribosomes before de novo synthesis meets temperature-induced demands. Reil1 reil2 double mutants accumulate 43S-preinitiation and pre-60S-maturation complexes and alter paralog composition of ribosomal proteins in non-translating complexes. With few exceptions, e.g. RPL3B and RPL24C, these changes are not under transcriptional control. Our study suggests requirement of de novo synthesis of eukaryotic ribosomes for long-term cold acclimation, feedback control of NUC2 and eIF3C2 transcription and links new proteins, AT1G03250, AT5G60530, to plant ribosome biogenesis. We propose that Arabidopsis requires biosynthesis of specialized ribosomes for cold acclimation.
format Article
author Cheong Bo Eng
Beine-Golovchuk, O.
Gorka, M.
Ho, W.W.H.
Martinez-Seidel, F.
Firmino, A.A.P.
Skirycz, A.
Roessner, U.
Kopka, J.
author_facet Cheong Bo Eng
Beine-Golovchuk, O.
Gorka, M.
Ho, W.W.H.
Martinez-Seidel, F.
Firmino, A.A.P.
Skirycz, A.
Roessner, U.
Kopka, J.
author_sort Cheong Bo Eng
title Arabidopsis REI-LIKE proteins activate ribosome biogenesis during cold acclimation
title_short Arabidopsis REI-LIKE proteins activate ribosome biogenesis during cold acclimation
title_full Arabidopsis REI-LIKE proteins activate ribosome biogenesis during cold acclimation
title_fullStr Arabidopsis REI-LIKE proteins activate ribosome biogenesis during cold acclimation
title_full_unstemmed Arabidopsis REI-LIKE proteins activate ribosome biogenesis during cold acclimation
title_sort arabidopsis rei-like proteins activate ribosome biogenesis during cold acclimation
publisher Nature Research
publishDate 2021
url https://eprints.ums.edu.my/id/eprint/26872/1/Arabidopsis%20REI-LIKE%20proteins%20activate%20ribosome%20biogenesis%20during%20cold%20acclimation.pdf
https://eprints.ums.edu.my/id/eprint/26872/
_version_ 1760230554447380480
score 13.211869

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