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Antioxidant properties of Carica Papaya leaves extracts prepared under different conditions and infused with “Kelulut” honey

Carica papaya is a tropical plant belonging to the Caricaceae family. Rich in phytochemical content, its leaves especially is renowned for its many health benefits especially for its anticancer properties as well as a supplement in treating dengue disease. This study thus aimed to optimise aqueous...

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Main Author: Monjia Belleza Cosmas Mojulat
Format: Thesis
Language:English
Published: Universiti Malaysia Sabah 2018
Subjects:
SF Animal culture
Online Access:https://eprints.ums.edu.my/id/eprint/26572/1/Antioxidant%20properties%20of%20Carica%20Papaya%20leaves%20extracts%20prepared%20under%20different%20conditions%20and%20infused%20with%20%E2%80%9CKelulut%E2%80%9D%20honey.pdf
https://eprints.ums.edu.my/id/eprint/26572/
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spelling my.ums.eprints.265722021-05-27T03:22:14Z https://eprints.ums.edu.my/id/eprint/26572/ Antioxidant properties of Carica Papaya leaves extracts prepared under different conditions and infused with “Kelulut” honey Monjia Belleza Cosmas Mojulat SF Animal culture Carica papaya is a tropical plant belonging to the Caricaceae family. Rich in phytochemical content, its leaves especially is renowned for its many health benefits especially for its anticancer properties as well as a supplement in treating dengue disease. This study thus aimed to optimise aqueous extraction and to determine the effect of honey infusion on the leaves extract which was analysed using TPC, TFC, FRAP and DPPH assays respectively. Based on analysis of 1 g of sample extracted with 100ml deionised water, the optimal extraction conditions for aqueous extraction were determined to be at 700C for 20 minutes where its TPC was 9.97+0.47 mg GAE/mL (p<0.05), TFC was totaled at 2.64+0.01 mg QUE/mL (p<0.05) while its FRAP assay was amounted to 16.84+1.10 mg TE/mL. Radical scavenging values using DPPH assay was recorded to be 87.53% with its IC50 at 492.54+2.45 mg TE/mL (p<0.05). Study on the infusion of “kelulut” honey with the leaves extract provides evidence that not only does it improve the taste of the bitter papaya extract; the positive synergy also increases the overall antioxidant activity. The aforementioned tests and assays recorded higher antioxidant activity with increasing honey dosage (max 4tbsp.), where its TPC was 21.66+0.54 mg GAE/mL (p<0.05), while antioxidant activity based on FRAP assay yielded 24.02+0.87 mg TE/mL (p<0.05). Accordingly, radical scavenging activity based on DPPH assay was 98.20% and lower IC50 value compared to infusion at lower dosage, amounting at 408.02+4.98 mg TE/mL. Subsequently, gallic acid and quercetin isolated from freeze dried Carica papaya leaves extract was higher compared to freshly prepared sample. However, conversely, gallic acid and quercetin yielded from fresh “kelulut” honey and Carica papaya leaves extract infused with “kelulut” honey is higher in fresh sample, suggesting that preservation effect using freeze dry method differ depending on sample type and method of preparation. Future study can therefore be done to improve and develop standardised preparation of infused papaya leaves with honey, and subsequently releasing it for commercial usage. Universiti Malaysia Sabah 2018 Thesis NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/26572/1/Antioxidant%20properties%20of%20Carica%20Papaya%20leaves%20extracts%20prepared%20under%20different%20conditions%20and%20infused%20with%20%E2%80%9CKelulut%E2%80%9D%20honey.pdf Monjia Belleza Cosmas Mojulat (2018) Antioxidant properties of Carica Papaya leaves extracts prepared under different conditions and infused with “Kelulut” honey. UNSPECIFIED thesis, Universiti Malaysia Sabah.
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
topic SF Animal culture
spellingShingle SF Animal culture
Monjia Belleza Cosmas Mojulat
Antioxidant properties of Carica Papaya leaves extracts prepared under different conditions and infused with “Kelulut” honey
description Carica papaya is a tropical plant belonging to the Caricaceae family. Rich in phytochemical content, its leaves especially is renowned for its many health benefits especially for its anticancer properties as well as a supplement in treating dengue disease. This study thus aimed to optimise aqueous extraction and to determine the effect of honey infusion on the leaves extract which was analysed using TPC, TFC, FRAP and DPPH assays respectively. Based on analysis of 1 g of sample extracted with 100ml deionised water, the optimal extraction conditions for aqueous extraction were determined to be at 700C for 20 minutes where its TPC was 9.97+0.47 mg GAE/mL (p<0.05), TFC was totaled at 2.64+0.01 mg QUE/mL (p<0.05) while its FRAP assay was amounted to 16.84+1.10 mg TE/mL. Radical scavenging values using DPPH assay was recorded to be 87.53% with its IC50 at 492.54+2.45 mg TE/mL (p<0.05). Study on the infusion of “kelulut” honey with the leaves extract provides evidence that not only does it improve the taste of the bitter papaya extract; the positive synergy also increases the overall antioxidant activity. The aforementioned tests and assays recorded higher antioxidant activity with increasing honey dosage (max 4tbsp.), where its TPC was 21.66+0.54 mg GAE/mL (p<0.05), while antioxidant activity based on FRAP assay yielded 24.02+0.87 mg TE/mL (p<0.05). Accordingly, radical scavenging activity based on DPPH assay was 98.20% and lower IC50 value compared to infusion at lower dosage, amounting at 408.02+4.98 mg TE/mL. Subsequently, gallic acid and quercetin isolated from freeze dried Carica papaya leaves extract was higher compared to freshly prepared sample. However, conversely, gallic acid and quercetin yielded from fresh “kelulut” honey and Carica papaya leaves extract infused with “kelulut” honey is higher in fresh sample, suggesting that preservation effect using freeze dry method differ depending on sample type and method of preparation. Future study can therefore be done to improve and develop standardised preparation of infused papaya leaves with honey, and subsequently releasing it for commercial usage.
format Thesis
author Monjia Belleza Cosmas Mojulat
author_facet Monjia Belleza Cosmas Mojulat
author_sort Monjia Belleza Cosmas Mojulat
title Antioxidant properties of Carica Papaya leaves extracts prepared under different conditions and infused with “Kelulut” honey
title_short Antioxidant properties of Carica Papaya leaves extracts prepared under different conditions and infused with “Kelulut” honey
title_full Antioxidant properties of Carica Papaya leaves extracts prepared under different conditions and infused with “Kelulut” honey
title_fullStr Antioxidant properties of Carica Papaya leaves extracts prepared under different conditions and infused with “Kelulut” honey
title_full_unstemmed Antioxidant properties of Carica Papaya leaves extracts prepared under different conditions and infused with “Kelulut” honey
title_sort antioxidant properties of carica papaya leaves extracts prepared under different conditions and infused with “kelulut” honey
publisher Universiti Malaysia Sabah
publishDate 2018
url https://eprints.ums.edu.my/id/eprint/26572/1/Antioxidant%20properties%20of%20Carica%20Papaya%20leaves%20extracts%20prepared%20under%20different%20conditions%20and%20infused%20with%20%E2%80%9CKelulut%E2%80%9D%20honey.pdf
https://eprints.ums.edu.my/id/eprint/26572/
_version_ 1760230516282359808
score 13.211869

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