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Effects of Elevated Temperature on the Tropical Soil Bacterial Diversity

Bacteria are important biological components of soil that play pivotal roles in improving soil quality and maintaining a balanced ecosystem. However, global climate change may have severe impacts on biodiversity and ecosystems including species loss and extinction of plants and animals, including mi...

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Main Authors: Chin Lai Mun, Clemente Michael Vui Ling Wong
Format: Article
Language:English
English
Subjects:
Q Science (General)
Online Access:https://eprints.ums.edu.my/id/eprint/26388/1/Effects%20of%20Elevated%20Temperature%20on%20the%20Tropical%20Soil%20Bacterial%20Diversity.pdf
https://eprints.ums.edu.my/id/eprint/26388/2/Effects%20of%20Elevated%20Temperature%20on%20the%20Tropical%20Soil%20Bacterial%20Diversity1.pdf
https://eprints.ums.edu.my/id/eprint/26388/
http://dx.doi.org/10.17576/jsm-2020-4910-01
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spelling my.ums.eprints.263882020-11-27T06:25:08Z https://eprints.ums.edu.my/id/eprint/26388/ Effects of Elevated Temperature on the Tropical Soil Bacterial Diversity Chin Lai Mun Clemente Michael Vui Ling Wong Q Science (General) Bacteria are important biological components of soil that play pivotal roles in improving soil quality and maintaining a balanced ecosystem. However, global climate change may have severe impacts on biodiversity and ecosystems including species loss and extinction of plants and animals, including microbes. Thus, it is crucial to determine how elevated temperature may alter soil bacterial diversity and composition. In this study, an in vitro simulated temperature rise experiment was carried out on soils from three sampling sites, referring to S1, S2, and S3 around Sabah, Malaysia. Soils were incubated at 25 °C (control) and 27 °C (simulated warming) with constant parameters in a growth chamber up to 16 months. Total DNA was extracted from microbes in the soil and used for PCR amplification targeting the V3-V4 region of the 16S rRNA gene. These amplicons were sequenced using the MiSeq platform (Illumina, USA). Raw DNA sequences were trimmed, merged, and aligned against the 16S rRNA sequences in the NCBI 16S database. The results showed that the analyzed soils were mainly dominated by Proteobacteria, Actinobacteria, Acidobacteria, and Verrucomicrobia. After 16 months of simulated warming, a net decrease of Proteobacteria, Acidobacteria, and Planctomycetes, and an increase of Actinobacteria and Chloroflexi were observed for all three soil samples, indicating that these phyla were highly affected by a temperature rise. At the genus level, Gaiella and Nocardioides exhibited a net increase while Bradyrhizobium, Mycobacterium, Tepidisphaera, and Paludibaculum demonstrated net decrease after 16 months of simulated warming. Knowledge on the changes of soil bacterial diversity patterns as a result of temperature elevation will contribute to select the best intervention strategy to overcome global warming issue in the future. Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/26388/1/Effects%20of%20Elevated%20Temperature%20on%20the%20Tropical%20Soil%20Bacterial%20Diversity.pdf text en https://eprints.ums.edu.my/id/eprint/26388/2/Effects%20of%20Elevated%20Temperature%20on%20the%20Tropical%20Soil%20Bacterial%20Diversity1.pdf Chin Lai Mun and Clemente Michael Vui Ling Wong Effects of Elevated Temperature on the Tropical Soil Bacterial Diversity. Sains Malaysiana, 49 (10). pp. 2335-2344. http://dx.doi.org/10.17576/jsm-2020-4910-01
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
English
topic Q Science (General)
spellingShingle Q Science (General)
Chin Lai Mun
Clemente Michael Vui Ling Wong
Effects of Elevated Temperature on the Tropical Soil Bacterial Diversity
description Bacteria are important biological components of soil that play pivotal roles in improving soil quality and maintaining a balanced ecosystem. However, global climate change may have severe impacts on biodiversity and ecosystems including species loss and extinction of plants and animals, including microbes. Thus, it is crucial to determine how elevated temperature may alter soil bacterial diversity and composition. In this study, an in vitro simulated temperature rise experiment was carried out on soils from three sampling sites, referring to S1, S2, and S3 around Sabah, Malaysia. Soils were incubated at 25 °C (control) and 27 °C (simulated warming) with constant parameters in a growth chamber up to 16 months. Total DNA was extracted from microbes in the soil and used for PCR amplification targeting the V3-V4 region of the 16S rRNA gene. These amplicons were sequenced using the MiSeq platform (Illumina, USA). Raw DNA sequences were trimmed, merged, and aligned against the 16S rRNA sequences in the NCBI 16S database. The results showed that the analyzed soils were mainly dominated by Proteobacteria, Actinobacteria, Acidobacteria, and Verrucomicrobia. After 16 months of simulated warming, a net decrease of Proteobacteria, Acidobacteria, and Planctomycetes, and an increase of Actinobacteria and Chloroflexi were observed for all three soil samples, indicating that these phyla were highly affected by a temperature rise. At the genus level, Gaiella and Nocardioides exhibited a net increase while Bradyrhizobium, Mycobacterium, Tepidisphaera, and Paludibaculum demonstrated net decrease after 16 months of simulated warming. Knowledge on the changes of soil bacterial diversity patterns as a result of temperature elevation will contribute to select the best intervention strategy to overcome global warming issue in the future.
format Article
author Chin Lai Mun
Clemente Michael Vui Ling Wong
author_facet Chin Lai Mun
Clemente Michael Vui Ling Wong
author_sort Chin Lai Mun
title Effects of Elevated Temperature on the Tropical Soil Bacterial Diversity
title_short Effects of Elevated Temperature on the Tropical Soil Bacterial Diversity
title_full Effects of Elevated Temperature on the Tropical Soil Bacterial Diversity
title_fullStr Effects of Elevated Temperature on the Tropical Soil Bacterial Diversity
title_full_unstemmed Effects of Elevated Temperature on the Tropical Soil Bacterial Diversity
title_sort effects of elevated temperature on the tropical soil bacterial diversity
url https://eprints.ums.edu.my/id/eprint/26388/1/Effects%20of%20Elevated%20Temperature%20on%20the%20Tropical%20Soil%20Bacterial%20Diversity.pdf
https://eprints.ums.edu.my/id/eprint/26388/2/Effects%20of%20Elevated%20Temperature%20on%20the%20Tropical%20Soil%20Bacterial%20Diversity1.pdf
https://eprints.ums.edu.my/id/eprint/26388/
http://dx.doi.org/10.17576/jsm-2020-4910-01
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score 13.211869

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