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Isolation, molecular characterization and antimicrobial susceptibility of Aeromonas spp. obtained from Tiger Grouper (Epinephelus fuscoguttatus) and Marble Goby (Oxyeleotris marmoratus) fish in Sabah, Malaysia

Aeromonads are ubiquitous in aquatic environments and have been implicated in fish and human infections. In this study, we isolated, studied antimicrobial susceptibility patterns and screened the existence of 15 virulence genes in aeromonads from two famously consumed fish species—seven marine Tiger...

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Main Authors: Tien‐Tien Vicky Lau, Suat‐Moi Puah, Ching, Kee Karen Hon, Ching, Fui Fui, Jin Ai Mary Anne Tan, Savithri Devi Ampalam Puthucheary, Lee, Ping Chin, Kek Heng Chua
Format: Article
Language:English
Published: 2020
Subjects:
Q Science (General)
SH Aquaculture. Fisheries. Angling
Online Access:https://eprints.ums.edu.my/id/eprint/26018/1/Isolation%2C%20molecular%20characterization%20and%20antimicrobial%20susceptibility%20of%20Aeromonas%20spp.%20obtained%20from%20Tiger%20Grouper%20%28Epinephelus%20fuscoguttatus%29%20and%20Marble%20Goby%20%28Oxyeleotris%20marmoratus%29%20fish%20in%20Sabah%2C%20Malaysia.pdf
https://eprints.ums.edu.my/id/eprint/26018/
https://doi.org/10.1111/are.14739
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spelling my.ums.eprints.260182020-09-24T05:40:24Z https://eprints.ums.edu.my/id/eprint/26018/ Isolation, molecular characterization and antimicrobial susceptibility of Aeromonas spp. obtained from Tiger Grouper (Epinephelus fuscoguttatus) and Marble Goby (Oxyeleotris marmoratus) fish in Sabah, Malaysia Tien‐Tien Vicky Lau Suat‐Moi Puah Ching, Kee Karen Hon Ching, Fui Fui Jin Ai Mary Anne Tan Savithri Devi Ampalam Puthucheary Lee, Ping Chin Kek Heng Chua Q Science (General) SH Aquaculture. Fisheries. Angling Aeromonads are ubiquitous in aquatic environments and have been implicated in fish and human infections. In this study, we isolated, studied antimicrobial susceptibility patterns and screened the existence of 15 virulence genes in aeromonads from two famously consumed fish species—seven marine Tiger Grouper (Epinephelus fuscoguttatus) and eight freshwater Marble Goby (Oxyeleotris marmoratus) from the aquaculture hatchery in Sabah, Malaysia. A total of 30 aeromonads (17 A. caviae, 9 A. rivuli, 4 A. dhakensis) were identified using PCR targeting GCAT gene, rpoD‐restriction fragment length polymorphism and multi‐locus phylogenetic analysis. All 30 strains were resistant to amoxicillin and cephalothin and five strains were multidrug‐resistant. Nine virulence genes (lip, ela, eno, fla, aerA, hylA, dam, alt and ser) present in A. dhakensis, suggesting the virulence potential of this species as a fish pathogen. This study offers as a baseline for future studies in monitoring and managing these two fish in aquaculture industry. 2020 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/26018/1/Isolation%2C%20molecular%20characterization%20and%20antimicrobial%20susceptibility%20of%20Aeromonas%20spp.%20obtained%20from%20Tiger%20Grouper%20%28Epinephelus%20fuscoguttatus%29%20and%20Marble%20Goby%20%28Oxyeleotris%20marmoratus%29%20fish%20in%20Sabah%2C%20Malaysia.pdf Tien‐Tien Vicky Lau and Suat‐Moi Puah and Ching, Kee Karen Hon and Ching, Fui Fui and Jin Ai Mary Anne Tan and Savithri Devi Ampalam Puthucheary and Lee, Ping Chin and Kek Heng Chua (2020) Isolation, molecular characterization and antimicrobial susceptibility of Aeromonas spp. obtained from Tiger Grouper (Epinephelus fuscoguttatus) and Marble Goby (Oxyeleotris marmoratus) fish in Sabah, Malaysia. Aquaculture Research, 51 (10). https://doi.org/10.1111/are.14739
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
topic Q Science (General)
SH Aquaculture. Fisheries. Angling
spellingShingle Q Science (General)
SH Aquaculture. Fisheries. Angling
Tien‐Tien Vicky Lau
Suat‐Moi Puah
Ching, Kee Karen Hon
Ching, Fui Fui
Jin Ai Mary Anne Tan
Savithri Devi Ampalam Puthucheary
Lee, Ping Chin
Kek Heng Chua
Isolation, molecular characterization and antimicrobial susceptibility of Aeromonas spp. obtained from Tiger Grouper (Epinephelus fuscoguttatus) and Marble Goby (Oxyeleotris marmoratus) fish in Sabah, Malaysia
description Aeromonads are ubiquitous in aquatic environments and have been implicated in fish and human infections. In this study, we isolated, studied antimicrobial susceptibility patterns and screened the existence of 15 virulence genes in aeromonads from two famously consumed fish species—seven marine Tiger Grouper (Epinephelus fuscoguttatus) and eight freshwater Marble Goby (Oxyeleotris marmoratus) from the aquaculture hatchery in Sabah, Malaysia. A total of 30 aeromonads (17 A. caviae, 9 A. rivuli, 4 A. dhakensis) were identified using PCR targeting GCAT gene, rpoD‐restriction fragment length polymorphism and multi‐locus phylogenetic analysis. All 30 strains were resistant to amoxicillin and cephalothin and five strains were multidrug‐resistant. Nine virulence genes (lip, ela, eno, fla, aerA, hylA, dam, alt and ser) present in A. dhakensis, suggesting the virulence potential of this species as a fish pathogen. This study offers as a baseline for future studies in monitoring and managing these two fish in aquaculture industry.
format Article
author Tien‐Tien Vicky Lau
Suat‐Moi Puah
Ching, Kee Karen Hon
Ching, Fui Fui
Jin Ai Mary Anne Tan
Savithri Devi Ampalam Puthucheary
Lee, Ping Chin
Kek Heng Chua
author_facet Tien‐Tien Vicky Lau
Suat‐Moi Puah
Ching, Kee Karen Hon
Ching, Fui Fui
Jin Ai Mary Anne Tan
Savithri Devi Ampalam Puthucheary
Lee, Ping Chin
Kek Heng Chua
author_sort Tien‐Tien Vicky Lau
title Isolation, molecular characterization and antimicrobial susceptibility of Aeromonas spp. obtained from Tiger Grouper (Epinephelus fuscoguttatus) and Marble Goby (Oxyeleotris marmoratus) fish in Sabah, Malaysia
title_short Isolation, molecular characterization and antimicrobial susceptibility of Aeromonas spp. obtained from Tiger Grouper (Epinephelus fuscoguttatus) and Marble Goby (Oxyeleotris marmoratus) fish in Sabah, Malaysia
title_full Isolation, molecular characterization and antimicrobial susceptibility of Aeromonas spp. obtained from Tiger Grouper (Epinephelus fuscoguttatus) and Marble Goby (Oxyeleotris marmoratus) fish in Sabah, Malaysia
title_fullStr Isolation, molecular characterization and antimicrobial susceptibility of Aeromonas spp. obtained from Tiger Grouper (Epinephelus fuscoguttatus) and Marble Goby (Oxyeleotris marmoratus) fish in Sabah, Malaysia
title_full_unstemmed Isolation, molecular characterization and antimicrobial susceptibility of Aeromonas spp. obtained from Tiger Grouper (Epinephelus fuscoguttatus) and Marble Goby (Oxyeleotris marmoratus) fish in Sabah, Malaysia
title_sort isolation, molecular characterization and antimicrobial susceptibility of aeromonas spp. obtained from tiger grouper (epinephelus fuscoguttatus) and marble goby (oxyeleotris marmoratus) fish in sabah, malaysia
publishDate 2020
url https://eprints.ums.edu.my/id/eprint/26018/1/Isolation%2C%20molecular%20characterization%20and%20antimicrobial%20susceptibility%20of%20Aeromonas%20spp.%20obtained%20from%20Tiger%20Grouper%20%28Epinephelus%20fuscoguttatus%29%20and%20Marble%20Goby%20%28Oxyeleotris%20marmoratus%29%20fish%20in%20Sabah%2C%20Malaysia.pdf
https://eprints.ums.edu.my/id/eprint/26018/
https://doi.org/10.1111/are.14739
_version_ 1760230444167593984
score 13.211869

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