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DNA markers for tuberculosis diagnosis

Tuberculosis (TB), caused by Mycobacterium tuberculosis complex (MTBC), is an infectious disease with more than 10.4 million cases and 1.7 million deaths reported worldwide in 2016. The classical methods for detection and differentiation of mycobacteria are: acid-fast microscopy (Ziehl-Neelsen stain...

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Main Authors:	Kai, Ling Chin, Maria E.Sarmiento, Mohd Nor Norazmi, Armando Acosta
Format:	Article
Language:	English
Published:	2018
Subjects:	RC Internal medicine
Online Access:	https://eprints.ums.edu.my/id/eprint/23760/1/DNA%20markers%20for%20tuberculosis%20diagnosis.pdf https://eprints.ums.edu.my/id/eprint/23760/ https://doi.org/10.1016/j.tube.2018.09.008
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spelling	my.ums.eprints.237602019-10-08T04:51:06Z https://eprints.ums.edu.my/id/eprint/23760/ DNA markers for tuberculosis diagnosis Kai, Ling Chin Maria E.Sarmiento Mohd Nor Norazmi Armando Acosta RC Internal medicine Tuberculosis (TB), caused by Mycobacterium tuberculosis complex (MTBC), is an infectious disease with more than 10.4 million cases and 1.7 million deaths reported worldwide in 2016. The classical methods for detection and differentiation of mycobacteria are: acid-fast microscopy (Ziehl-Neelsen staining), culture, and biochemical methods. However, the microbial phenotypic characterization is time-consuming and laborious. Thus, fast, easy, and sensitive nucleic acid amplification tests (NAATs) have been developed based on specific DNA markers, which are commercially available for TB diagnosis. Despite these developments, the disease remains uncontrollable. The identification and differentiation among MTBC members with the use of NAATs remains challenging due, among other factors, to the high degree of homology within the members and mutations, which hinders the identification of specific target sequences in the genome with potential impact in the diagnosis and treatment outcomes. In silico methods provide predictive identification of many new target genes/fragments/regions that can specifically be used to identify species/strains, which have not been fully explored. This review focused on DNA markers useful for MTBC detection, species identification and antibiotic resistance determination. The use of DNA targets with new technological approaches will help to develop NAATs applicable to all levels of the health system, mainly in low resource areas, which urgently need customized methods to their specific conditions. 2018-12 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/23760/1/DNA%20markers%20for%20tuberculosis%20diagnosis.pdf Kai, Ling Chin and Maria E.Sarmiento and Mohd Nor Norazmi and Armando Acosta (2018) DNA markers for tuberculosis diagnosis. Tuberculosis, 113. pp. 139-152. https://doi.org/10.1016/j.tube.2018.09.008
institution	Universiti Malaysia Sabah
building	UMS Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaysia Sabah
content_source	UMS Institutional Repository
url_provider	http://eprints.ums.edu.my/
language	English
topic	RC Internal medicine
spellingShingle	RC Internal medicine Kai, Ling Chin Maria E.Sarmiento Mohd Nor Norazmi Armando Acosta DNA markers for tuberculosis diagnosis
description	Tuberculosis (TB), caused by Mycobacterium tuberculosis complex (MTBC), is an infectious disease with more than 10.4 million cases and 1.7 million deaths reported worldwide in 2016. The classical methods for detection and differentiation of mycobacteria are: acid-fast microscopy (Ziehl-Neelsen staining), culture, and biochemical methods. However, the microbial phenotypic characterization is time-consuming and laborious. Thus, fast, easy, and sensitive nucleic acid amplification tests (NAATs) have been developed based on specific DNA markers, which are commercially available for TB diagnosis. Despite these developments, the disease remains uncontrollable. The identification and differentiation among MTBC members with the use of NAATs remains challenging due, among other factors, to the high degree of homology within the members and mutations, which hinders the identification of specific target sequences in the genome with potential impact in the diagnosis and treatment outcomes. In silico methods provide predictive identification of many new target genes/fragments/regions that can specifically be used to identify species/strains, which have not been fully explored. This review focused on DNA markers useful for MTBC detection, species identification and antibiotic resistance determination. The use of DNA targets with new technological approaches will help to develop NAATs applicable to all levels of the health system, mainly in low resource areas, which urgently need customized methods to their specific conditions.
format	Article
author	Kai, Ling Chin Maria E.Sarmiento Mohd Nor Norazmi Armando Acosta
author_facet	Kai, Ling Chin Maria E.Sarmiento Mohd Nor Norazmi Armando Acosta
author_sort	Kai, Ling Chin
title	DNA markers for tuberculosis diagnosis
title_short	DNA markers for tuberculosis diagnosis
title_full	DNA markers for tuberculosis diagnosis
title_fullStr	DNA markers for tuberculosis diagnosis
title_full_unstemmed	DNA markers for tuberculosis diagnosis
title_sort	dna markers for tuberculosis diagnosis
publishDate	2018
url	https://eprints.ums.edu.my/id/eprint/23760/1/DNA%20markers%20for%20tuberculosis%20diagnosis.pdf https://eprints.ums.edu.my/id/eprint/23760/ https://doi.org/10.1016/j.tube.2018.09.008
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score	13.211869

DNA markers for tuberculosis diagnosis

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