Staff View: Mitochondrial variation in subpopulations of Anopheles balabacensis Baisas in Sabah, Malaysia (Diptera: Culicidae)

Mitochondrial variation in subpopulations of Anopheles balabacensis Baisas in Sabah, Malaysia (Diptera: Culicidae)

Anopheles balabacensis, the primary vector of Plasmodium knowlesi in Sabah, Malaysia, is both zoophilic and anthropophilic, feeding on macaques as well as humans. It is the dominant Anopheles species found in Kudat Division where it is responsible for all the cases of P. knowlesi. However there is a...

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Main Authors:	Benny Obrain Manin, Chris J. Drakeley, Chua, Tock Hing
Format:	Article
Language:	English
Published:	2018
Subjects:	RC Internal medicine
Online Access:	https://eprints.ums.edu.my/id/eprint/22568/1/Mitochondrial%20variation%20in%20subpopulations%20of%20Anopheles%20balabacensis%20Baisas%20in%20Sabah.pdf https://eprints.ums.edu.my/id/eprint/22568/ https://doi.org/10.1371/journal.pone.0202905
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spelling	my.ums.eprints.225682019-07-11T07:40:47Z https://eprints.ums.edu.my/id/eprint/22568/ Mitochondrial variation in subpopulations of Anopheles balabacensis Baisas in Sabah, Malaysia (Diptera: Culicidae) Benny Obrain Manin Chris J. Drakeley Chua, Tock Hing RC Internal medicine Anopheles balabacensis, the primary vector of Plasmodium knowlesi in Sabah, Malaysia, is both zoophilic and anthropophilic, feeding on macaques as well as humans. It is the dominant Anopheles species found in Kudat Division where it is responsible for all the cases of P. knowlesi. However there is a paucity of basic biological and ecological information on this vector. We investigated the genetic variation of this species using the sequences of cox1 (1,383 bp) and cox2 (685 bp) to gain an insight into the population genetics and inter-population gene flow in Sabah. A total of 71 An. balabacensis were collected from seven districts constituting 14 subpopulations. A total of 17, 10 and 25 haplotypes were detected in the subpopulations respectively using the cox1, cox2 and the combined sequence. Some of the haplotypes were common among the subpopulations due to gene flow occurring between them. AMOVA showed that the genetic variation was high within subpopulations as compared to between subpopulations. Mantel test results showed that the variation between subpopulations was not due to the geographical distance between them. Furthermore, Tajima’s D and Fu’s Fs tests showed that An. balabacensis in Sabah is experiencing population expansion and growth. High gene flow between the subpopulations was indicated by the low genetic distance and high gene diversity in the cox1, cox2 and the combined sequence. However the population at Lipasu Lama appeared to be isolated possibly due to its higher altitude at 873 m above sea level. 2018-08-23 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/22568/1/Mitochondrial%20variation%20in%20subpopulations%20of%20Anopheles%20balabacensis%20Baisas%20in%20Sabah.pdf Benny Obrain Manin and Chris J. Drakeley and Chua, Tock Hing (2018) Mitochondrial variation in subpopulations of Anopheles balabacensis Baisas in Sabah, Malaysia (Diptera: Culicidae). Plos One. pp. 1-17. ISSN 1932-6203 https://doi.org/10.1371/journal.pone.0202905
institution	Universiti Malaysia Sabah
building	UMS Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaysia Sabah
content_source	UMS Institutional Repository
url_provider	http://eprints.ums.edu.my/
language	English
topic	RC Internal medicine
spellingShingle	RC Internal medicine Benny Obrain Manin Chris J. Drakeley Chua, Tock Hing Mitochondrial variation in subpopulations of Anopheles balabacensis Baisas in Sabah, Malaysia (Diptera: Culicidae)
description	Anopheles balabacensis, the primary vector of Plasmodium knowlesi in Sabah, Malaysia, is both zoophilic and anthropophilic, feeding on macaques as well as humans. It is the dominant Anopheles species found in Kudat Division where it is responsible for all the cases of P. knowlesi. However there is a paucity of basic biological and ecological information on this vector. We investigated the genetic variation of this species using the sequences of cox1 (1,383 bp) and cox2 (685 bp) to gain an insight into the population genetics and inter-population gene flow in Sabah. A total of 71 An. balabacensis were collected from seven districts constituting 14 subpopulations. A total of 17, 10 and 25 haplotypes were detected in the subpopulations respectively using the cox1, cox2 and the combined sequence. Some of the haplotypes were common among the subpopulations due to gene flow occurring between them. AMOVA showed that the genetic variation was high within subpopulations as compared to between subpopulations. Mantel test results showed that the variation between subpopulations was not due to the geographical distance between them. Furthermore, Tajima’s D and Fu’s Fs tests showed that An. balabacensis in Sabah is experiencing population expansion and growth. High gene flow between the subpopulations was indicated by the low genetic distance and high gene diversity in the cox1, cox2 and the combined sequence. However the population at Lipasu Lama appeared to be isolated possibly due to its higher altitude at 873 m above sea level.
format	Article
author	Benny Obrain Manin Chris J. Drakeley Chua, Tock Hing
author_facet	Benny Obrain Manin Chris J. Drakeley Chua, Tock Hing
author_sort	Benny Obrain Manin
title	Mitochondrial variation in subpopulations of Anopheles balabacensis Baisas in Sabah, Malaysia (Diptera: Culicidae)
title_short	Mitochondrial variation in subpopulations of Anopheles balabacensis Baisas in Sabah, Malaysia (Diptera: Culicidae)
title_full	Mitochondrial variation in subpopulations of Anopheles balabacensis Baisas in Sabah, Malaysia (Diptera: Culicidae)
title_fullStr	Mitochondrial variation in subpopulations of Anopheles balabacensis Baisas in Sabah, Malaysia (Diptera: Culicidae)
title_full_unstemmed	Mitochondrial variation in subpopulations of Anopheles balabacensis Baisas in Sabah, Malaysia (Diptera: Culicidae)
title_sort	mitochondrial variation in subpopulations of anopheles balabacensis baisas in sabah, malaysia (diptera: culicidae)
publishDate	2018
url	https://eprints.ums.edu.my/id/eprint/22568/1/Mitochondrial%20variation%20in%20subpopulations%20of%20Anopheles%20balabacensis%20Baisas%20in%20Sabah.pdf https://eprints.ums.edu.my/id/eprint/22568/ https://doi.org/10.1371/journal.pone.0202905
_version_	1760229986237677568
score	13.211869

Mitochondrial variation in subpopulations of Anopheles balabacensis Baisas in Sabah, Malaysia (Diptera: Culicidae)

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