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Isolation and Characterization of Surface and Subsurface Bacteria in Seawater of Mantanani Island, Kota Belud, Sabah by Direct and Enrichment Techniques

The distribution of hydrocarbon-utilizing bacterial may vary between surface and subsurface of the seawater. One of the identified contributors is the Total Petroleum Hydrocarbon. The isolation and characterization of bacteria using Direct and Enrichment techniques helps in identifying dominant bact...

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Main Authors: Benard, L D, Tuah, P.M, Suadin, E G, Jamian, N
Format: Article
Language:English
English
Published: 78(1) · April 2015 2015
Online Access:https://eprints.ums.edu.my/id/eprint/21978/1/Isolation%20and%20Characterization%20of%20Surface%20and%20Subsurface%20Bacteria%20in%20Seawater%20of%20Mantanani%20Island.pdf
https://eprints.ums.edu.my/id/eprint/21978/7/Isolation%20and%20Characterization%20of%20Surface%20and%20Subsurface%20Bacteria%20in%20Seawater%20of%20Mantanani%20Island%2C%20Kota%20Belud%2C%20Sabah%20by%20Direct%20and%20Enrichment%20Techniques..pdf
https://eprints.ums.edu.my/id/eprint/21978/
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spelling my.ums.eprints.219782021-04-30T07:53:42Z https://eprints.ums.edu.my/id/eprint/21978/ Isolation and Characterization of Surface and Subsurface Bacteria in Seawater of Mantanani Island, Kota Belud, Sabah by Direct and Enrichment Techniques Benard, L D Tuah, P.M Suadin, E G Jamian, N The distribution of hydrocarbon-utilizing bacterial may vary between surface and subsurface of the seawater. One of the identified contributors is the Total Petroleum Hydrocarbon. The isolation and characterization of bacteria using Direct and Enrichment techniques helps in identifying dominant bacterial populations in seawater of Mantanani Island, Kota Belud, Sabah, potential of further investigation as hydrocarbon degrader. Crude oil (5% v/v) was added as the carbon source for bacteria in Enrichment technique. For surface seawater, the highest population of bacteria identified for both Direct and Enrichment technique were 2.60 × 107 CFU/mL and 3.84 × 106 CFU/mL respectively. Meanwhile, for subsurface seawater, the highest population of bacteria identified for both Direct and Enrichment technique were 5.21 × 106 CFU/mL and 8.99 × 107 CFU/mL respectively. Dominant species in surface seawater were characterized as Marinobacter hydrocarbonoclasticus-RMSF-C1 and RMSF-C2 and Alcanivorax borkumensis-RMSF-C3, RMSF-C4 and RMSF-C5. As for subsurface seawater, dominant species were characterized as Pseudomonas luteola-SSBR-W1, Burkholderia cepacia-SSBR-C1, Rhizobium radiobacter- SSBR-C3 and Leuconostoc-cremois -SSBR-C4. 78(1) · April 2015 2015 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/21978/1/Isolation%20and%20Characterization%20of%20Surface%20and%20Subsurface%20Bacteria%20in%20Seawater%20of%20Mantanani%20Island.pdf text en https://eprints.ums.edu.my/id/eprint/21978/7/Isolation%20and%20Characterization%20of%20Surface%20and%20Subsurface%20Bacteria%20in%20Seawater%20of%20Mantanani%20Island%2C%20Kota%20Belud%2C%20Sabah%20by%20Direct%20and%20Enrichment%20Techniques..pdf Benard, L D and Tuah, P.M and Suadin, E G and Jamian, N (2015) Isolation and Characterization of Surface and Subsurface Bacteria in Seawater of Mantanani Island, Kota Belud, Sabah by Direct and Enrichment Techniques. Materials Science & Engineering, 78 (1). DOI: 10.1088/1757-899X/78/1/012033
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
English
description The distribution of hydrocarbon-utilizing bacterial may vary between surface and subsurface of the seawater. One of the identified contributors is the Total Petroleum Hydrocarbon. The isolation and characterization of bacteria using Direct and Enrichment techniques helps in identifying dominant bacterial populations in seawater of Mantanani Island, Kota Belud, Sabah, potential of further investigation as hydrocarbon degrader. Crude oil (5% v/v) was added as the carbon source for bacteria in Enrichment technique. For surface seawater, the highest population of bacteria identified for both Direct and Enrichment technique were 2.60 × 107 CFU/mL and 3.84 × 106 CFU/mL respectively. Meanwhile, for subsurface seawater, the highest population of bacteria identified for both Direct and Enrichment technique were 5.21 × 106 CFU/mL and 8.99 × 107 CFU/mL respectively. Dominant species in surface seawater were characterized as Marinobacter hydrocarbonoclasticus-RMSF-C1 and RMSF-C2 and Alcanivorax borkumensis-RMSF-C3, RMSF-C4 and RMSF-C5. As for subsurface seawater, dominant species were characterized as Pseudomonas luteola-SSBR-W1, Burkholderia cepacia-SSBR-C1, Rhizobium radiobacter- SSBR-C3 and Leuconostoc-cremois -SSBR-C4.
format Article
author Benard, L D
Tuah, P.M
Suadin, E G
Jamian, N
spellingShingle Benard, L D
Tuah, P.M
Suadin, E G
Jamian, N
Isolation and Characterization of Surface and Subsurface Bacteria in Seawater of Mantanani Island, Kota Belud, Sabah by Direct and Enrichment Techniques
author_facet Benard, L D
Tuah, P.M
Suadin, E G
Jamian, N
author_sort Benard, L D
title Isolation and Characterization of Surface and Subsurface Bacteria in Seawater of Mantanani Island, Kota Belud, Sabah by Direct and Enrichment Techniques
title_short Isolation and Characterization of Surface and Subsurface Bacteria in Seawater of Mantanani Island, Kota Belud, Sabah by Direct and Enrichment Techniques
title_full Isolation and Characterization of Surface and Subsurface Bacteria in Seawater of Mantanani Island, Kota Belud, Sabah by Direct and Enrichment Techniques
title_fullStr Isolation and Characterization of Surface and Subsurface Bacteria in Seawater of Mantanani Island, Kota Belud, Sabah by Direct and Enrichment Techniques
title_full_unstemmed Isolation and Characterization of Surface and Subsurface Bacteria in Seawater of Mantanani Island, Kota Belud, Sabah by Direct and Enrichment Techniques
title_sort isolation and characterization of surface and subsurface bacteria in seawater of mantanani island, kota belud, sabah by direct and enrichment techniques
publisher 78(1) · April 2015
publishDate 2015
url https://eprints.ums.edu.my/id/eprint/21978/1/Isolation%20and%20Characterization%20of%20Surface%20and%20Subsurface%20Bacteria%20in%20Seawater%20of%20Mantanani%20Island.pdf
https://eprints.ums.edu.my/id/eprint/21978/7/Isolation%20and%20Characterization%20of%20Surface%20and%20Subsurface%20Bacteria%20in%20Seawater%20of%20Mantanani%20Island%2C%20Kota%20Belud%2C%20Sabah%20by%20Direct%20and%20Enrichment%20Techniques..pdf
https://eprints.ums.edu.my/id/eprint/21978/
_version_ 1760229910882811904
score 13.211869

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