Cover Image

Variant Alleles in XRCC1 Arg194Trp and Arg399Gln Polymorphisms Increase Risk of Gastrointestinal Cancer in Sabah, North Borneo

Background: The XRCC1 protein facilitates various DNA repair pathways; single-nucleotide polymorphisms (SNPs) in this gene are associated with a risk of gastrointestinal cancer (GIC) with inconsistent results, but no data have been previously reported for the Sabah, North Borneo, population. We acco...

Full description

Saved in:
Bibliographic Details
Main Authors: Noor Hanis Abu Halim, Tzyy, Eric Jiann Chong, Poh, Lucky Wah Goh, Jitt, Aun Chuah, Un, Edwin Hean See, Chua, Kek Heng, Lee, Ping Chin
Format: Article
Language:English
English
Published: Korea science 2016
Subjects:
RC Internal medicine
Online Access:https://eprints.ums.edu.my/id/eprint/21613/1/Variant%20Alleles%20in%20XRCC1%20Arg194Trp%20and%20Arg399Gln%20Polymorphisms%20Increase%20Risk%20of%20Gastrointestinal%20Cancer%20in%20Sabah%2C%20North%20Borneo.pdf
https://eprints.ums.edu.my/id/eprint/21613/7/Variant%20Alleles%20in%20XRCC1%20Arg194Trp%20and%20Arg399Gln.pdf
https://eprints.ums.edu.my/id/eprint/21613/
http://dx.doi.org/10.7314/APJCP.2016.17.4.1925
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.ums.eprints.21613
record_format eprints
spelling my.ums.eprints.216132020-12-28T04:26:28Z https://eprints.ums.edu.my/id/eprint/21613/ Variant Alleles in XRCC1 Arg194Trp and Arg399Gln Polymorphisms Increase Risk of Gastrointestinal Cancer in Sabah, North Borneo Noor Hanis Abu Halim Tzyy, Eric Jiann Chong Poh, Lucky Wah Goh Jitt, Aun Chuah Un, Edwin Hean See Chua, Kek Heng Lee, Ping Chin RC Internal medicine Background: The XRCC1 protein facilitates various DNA repair pathways; single-nucleotide polymorphisms (SNPs) in this gene are associated with a risk of gastrointestinal cancer (GIC) with inconsistent results, but no data have been previously reported for the Sabah, North Borneo, population. We accordingly investigated the XRCC1 Arg194Trp and Arg399Gln SNPs in terms of GIC risk in Sabah. Materials and Methods: We performed genotyping for both SNPs for 250 GIC patients and 572 healthy volunteers using a polymerase chain reaction-restriction fragment length polymorphism approach. We validated heterozygosity and homozygosity for both SNPs using direct sequencing. Results: The presence of a variant 194Trp allele in the Arg194Trp SNP was significantly associated with a higher risk of GIC, especially with gastric and colorectal cancers. We additionally found that the variant 399Gln allele in Arg399Gln SNP was associated with a greater risk of developing gastric cancer. Our combined analysis revealed that inheritance of variant alleles in both SNPs increased the GIC risk in Sabah population. Based on our etiological analysis, we found that subjects ≥50years≥50yearsand males who carrying the variant 194Trp allele, and Bajau subjects carrying the 399Gln allele had a significantly increased risk of GIC. Conclusions: Our findings suggest that inheritance of variant alleles in XRCC1 Arg194Trp and Arg399Gln SNPs may act as biomarkers for the early detection of GIC, especially for gastric and colorectal cancers in the Sabah population. Korea science 2016 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/21613/1/Variant%20Alleles%20in%20XRCC1%20Arg194Trp%20and%20Arg399Gln%20Polymorphisms%20Increase%20Risk%20of%20Gastrointestinal%20Cancer%20in%20Sabah%2C%20North%20Borneo.pdf text en https://eprints.ums.edu.my/id/eprint/21613/7/Variant%20Alleles%20in%20XRCC1%20Arg194Trp%20and%20Arg399Gln.pdf Noor Hanis Abu Halim and Tzyy, Eric Jiann Chong and Poh, Lucky Wah Goh and Jitt, Aun Chuah and Un, Edwin Hean See and Chua, Kek Heng and Lee, Ping Chin (2016) Variant Alleles in XRCC1 Arg194Trp and Arg399Gln Polymorphisms Increase Risk of Gastrointestinal Cancer in Sabah, North Borneo. Asia Pacific Journal of Cancer Prevention, 17 (4). pp. 1925-1931. ISSN 1513-7368 http://dx.doi.org/10.7314/APJCP.2016.17.4.1925
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
English
topic RC Internal medicine
spellingShingle RC Internal medicine
Noor Hanis Abu Halim
Tzyy, Eric Jiann Chong
Poh, Lucky Wah Goh
Jitt, Aun Chuah
Un, Edwin Hean See
Chua, Kek Heng
Lee, Ping Chin
Variant Alleles in XRCC1 Arg194Trp and Arg399Gln Polymorphisms Increase Risk of Gastrointestinal Cancer in Sabah, North Borneo
description Background: The XRCC1 protein facilitates various DNA repair pathways; single-nucleotide polymorphisms (SNPs) in this gene are associated with a risk of gastrointestinal cancer (GIC) with inconsistent results, but no data have been previously reported for the Sabah, North Borneo, population. We accordingly investigated the XRCC1 Arg194Trp and Arg399Gln SNPs in terms of GIC risk in Sabah. Materials and Methods: We performed genotyping for both SNPs for 250 GIC patients and 572 healthy volunteers using a polymerase chain reaction-restriction fragment length polymorphism approach. We validated heterozygosity and homozygosity for both SNPs using direct sequencing. Results: The presence of a variant 194Trp allele in the Arg194Trp SNP was significantly associated with a higher risk of GIC, especially with gastric and colorectal cancers. We additionally found that the variant 399Gln allele in Arg399Gln SNP was associated with a greater risk of developing gastric cancer. Our combined analysis revealed that inheritance of variant alleles in both SNPs increased the GIC risk in Sabah population. Based on our etiological analysis, we found that subjects ≥50years≥50yearsand males who carrying the variant 194Trp allele, and Bajau subjects carrying the 399Gln allele had a significantly increased risk of GIC. Conclusions: Our findings suggest that inheritance of variant alleles in XRCC1 Arg194Trp and Arg399Gln SNPs may act as biomarkers for the early detection of GIC, especially for gastric and colorectal cancers in the Sabah population.
format Article
author Noor Hanis Abu Halim
Tzyy, Eric Jiann Chong
Poh, Lucky Wah Goh
Jitt, Aun Chuah
Un, Edwin Hean See
Chua, Kek Heng
Lee, Ping Chin
author_facet Noor Hanis Abu Halim
Tzyy, Eric Jiann Chong
Poh, Lucky Wah Goh
Jitt, Aun Chuah
Un, Edwin Hean See
Chua, Kek Heng
Lee, Ping Chin
author_sort Noor Hanis Abu Halim
title Variant Alleles in XRCC1 Arg194Trp and Arg399Gln Polymorphisms Increase Risk of Gastrointestinal Cancer in Sabah, North Borneo
title_short Variant Alleles in XRCC1 Arg194Trp and Arg399Gln Polymorphisms Increase Risk of Gastrointestinal Cancer in Sabah, North Borneo
title_full Variant Alleles in XRCC1 Arg194Trp and Arg399Gln Polymorphisms Increase Risk of Gastrointestinal Cancer in Sabah, North Borneo
title_fullStr Variant Alleles in XRCC1 Arg194Trp and Arg399Gln Polymorphisms Increase Risk of Gastrointestinal Cancer in Sabah, North Borneo
title_full_unstemmed Variant Alleles in XRCC1 Arg194Trp and Arg399Gln Polymorphisms Increase Risk of Gastrointestinal Cancer in Sabah, North Borneo
title_sort variant alleles in xrcc1 arg194trp and arg399gln polymorphisms increase risk of gastrointestinal cancer in sabah, north borneo
publisher Korea science
publishDate 2016
url https://eprints.ums.edu.my/id/eprint/21613/1/Variant%20Alleles%20in%20XRCC1%20Arg194Trp%20and%20Arg399Gln%20Polymorphisms%20Increase%20Risk%20of%20Gastrointestinal%20Cancer%20in%20Sabah%2C%20North%20Borneo.pdf
https://eprints.ums.edu.my/id/eprint/21613/7/Variant%20Alleles%20in%20XRCC1%20Arg194Trp%20and%20Arg399Gln.pdf
https://eprints.ums.edu.my/id/eprint/21613/
http://dx.doi.org/10.7314/APJCP.2016.17.4.1925
_version_ 1760229865431236608
score 13.211869

Similar Items