Staff View: Hypoglycemic effects of gynura procumbens fractions on streptozotocin-induced diabetic rats involved phosphorylation of GSK3β (Ser-9) in liver

Hypoglycemic effects of gynura procumbens fractions on streptozotocin-induced diabetic rats involved phosphorylation of GSK3β (Ser-9) in liver

Gynura procumbens, a medicinal plant locally known as Sambung nyawa, is widely used traditionally in South East Asia as a remedy to alleviate symptoms of various ailments including diabetes mellitus. Previous investigators reported that the ethanol plant extract was hypoglycemic in streptozotocin (S...

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Main Authors:	Chong, Ching June, Lee, Hui Wen, Halimah Abdullah Sani, Jalifah Latip, Jualang Azlan Gansau, Lee, Ping Chin, Noor Embi, Hasidah Mohd Sidek
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2012
Subjects:	RC Internal medicine
Online Access:	https://eprints.ums.edu.my/id/eprint/20585/1/Hypoglycemic%20effects%20of%20gynura%20procumbens%20fractions%20on%20streptozotocin.pdf https://eprints.ums.edu.my/id/eprint/20585/ https://www.researchgate.net/publication/236030950_Hypoglycemic_effects_of_Gynura_procumbens_fractions_on_streptozotocin-induced_diabetic_rats_involved_phosphorylation_of_GSK3b_Ser-9_in_liver
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spelling	my.ums.eprints.205852018-08-06T08:28:21Z https://eprints.ums.edu.my/id/eprint/20585/ Hypoglycemic effects of gynura procumbens fractions on streptozotocin-induced diabetic rats involved phosphorylation of GSK3β (Ser-9) in liver Chong, Ching June Lee, Hui Wen Halimah Abdullah Sani Jalifah Latip Jualang Azlan Gansau Lee, Ping Chin Noor Embi Hasidah Mohd Sidek RC Internal medicine Gynura procumbens, a medicinal plant locally known as Sambung nyawa, is widely used traditionally in South East Asia as a remedy to alleviate symptoms of various ailments including diabetes mellitus. Previous investigators reported that the ethanol plant extract was hypoglycemic in streptozotocin (STZ)-induced diabetic rats and suggested the blood glucose-reducing effect could be due to G. procumbens mimicking insulin effects. Our present study aimed to reevaluate the hypoglycemic effect(s) of G. procumbens and to ascertain the involvement of glycogen synthase kinase (GSK3), a key component of insulin biosignaling in the plant activity. A cell-based assay showed that the three G. procumbens fractions tested did not exhibit anti-GSK3 activities. Oral administrations of hexane, ethyl acetate, n-butanol fractions of G. procumbens (250 mg/kg b.w.) and Glibenclamide (5 mg/kg b.w.) for 14 days in STZ-induced diabetic rats reduced blood glucose levels by 29.7%, 60.1%, 33.5% and 61.7% (p<0.05), respectively compared to diabetic control. Liver glycogen contents were elevated (p<0.05), following administration of the three fractions of G. procumbens in diabetic rats when analysed after 14 days of treatment. Western blot analysis also showed GSK3β in the liver of G. procumbens fractions-treated and Glibenclamide-treated animals were phosphorylated at Ser-9. The above ndings indicated that the hypoglycemic action of G. procumbens fractions resulted in the inactivation of GSK3β in liver of diabetic rats. Since G. procumbens fractions did not exhibit GSK3 inhibitory properties, it is possible that the hypoglycemic action of the fractions observed here could be due to direct or indirect effects on upstream components of the insulin biosignaling pathway. Universiti Kebangsaan Malaysia 2012 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/20585/1/Hypoglycemic%20effects%20of%20gynura%20procumbens%20fractions%20on%20streptozotocin.pdf Chong, Ching June and Lee, Hui Wen and Halimah Abdullah Sani and Jalifah Latip and Jualang Azlan Gansau and Lee, Ping Chin and Noor Embi and Hasidah Mohd Sidek (2012) Hypoglycemic effects of gynura procumbens fractions on streptozotocin-induced diabetic rats involved phosphorylation of GSK3β (Ser-9) in liver. Sains Malaysiana, 41 (8). pp. 969-975. ISSN 0126-6039 https://www.researchgate.net/publication/236030950_Hypoglycemic_effects_of_Gynura_procumbens_fractions_on_streptozotocin-induced_diabetic_rats_involved_phosphorylation_of_GSK3b_Ser-9_in_liver
institution	Universiti Malaysia Sabah
building	UMS Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaysia Sabah
content_source	UMS Institutional Repository
url_provider	http://eprints.ums.edu.my/
language	English
topic	RC Internal medicine
spellingShingle	RC Internal medicine Chong, Ching June Lee, Hui Wen Halimah Abdullah Sani Jalifah Latip Jualang Azlan Gansau Lee, Ping Chin Noor Embi Hasidah Mohd Sidek Hypoglycemic effects of gynura procumbens fractions on streptozotocin-induced diabetic rats involved phosphorylation of GSK3β (Ser-9) in liver
description	Gynura procumbens, a medicinal plant locally known as Sambung nyawa, is widely used traditionally in South East Asia as a remedy to alleviate symptoms of various ailments including diabetes mellitus. Previous investigators reported that the ethanol plant extract was hypoglycemic in streptozotocin (STZ)-induced diabetic rats and suggested the blood glucose-reducing effect could be due to G. procumbens mimicking insulin effects. Our present study aimed to reevaluate the hypoglycemic effect(s) of G. procumbens and to ascertain the involvement of glycogen synthase kinase (GSK3), a key component of insulin biosignaling in the plant activity. A cell-based assay showed that the three G. procumbens fractions tested did not exhibit anti-GSK3 activities. Oral administrations of hexane, ethyl acetate, n-butanol fractions of G. procumbens (250 mg/kg b.w.) and Glibenclamide (5 mg/kg b.w.) for 14 days in STZ-induced diabetic rats reduced blood glucose levels by 29.7%, 60.1%, 33.5% and 61.7% (p<0.05), respectively compared to diabetic control. Liver glycogen contents were elevated (p<0.05), following administration of the three fractions of G. procumbens in diabetic rats when analysed after 14 days of treatment. Western blot analysis also showed GSK3β in the liver of G. procumbens fractions-treated and Glibenclamide-treated animals were phosphorylated at Ser-9. The above ndings indicated that the hypoglycemic action of G. procumbens fractions resulted in the inactivation of GSK3β in liver of diabetic rats. Since G. procumbens fractions did not exhibit GSK3 inhibitory properties, it is possible that the hypoglycemic action of the fractions observed here could be due to direct or indirect effects on upstream components of the insulin biosignaling pathway.
format	Article
author	Chong, Ching June Lee, Hui Wen Halimah Abdullah Sani Jalifah Latip Jualang Azlan Gansau Lee, Ping Chin Noor Embi Hasidah Mohd Sidek
author_facet	Chong, Ching June Lee, Hui Wen Halimah Abdullah Sani Jalifah Latip Jualang Azlan Gansau Lee, Ping Chin Noor Embi Hasidah Mohd Sidek
author_sort	Chong, Ching June
title	Hypoglycemic effects of gynura procumbens fractions on streptozotocin-induced diabetic rats involved phosphorylation of GSK3β (Ser-9) in liver
title_short	Hypoglycemic effects of gynura procumbens fractions on streptozotocin-induced diabetic rats involved phosphorylation of GSK3β (Ser-9) in liver
title_full	Hypoglycemic effects of gynura procumbens fractions on streptozotocin-induced diabetic rats involved phosphorylation of GSK3β (Ser-9) in liver
title_fullStr	Hypoglycemic effects of gynura procumbens fractions on streptozotocin-induced diabetic rats involved phosphorylation of GSK3β (Ser-9) in liver
title_full_unstemmed	Hypoglycemic effects of gynura procumbens fractions on streptozotocin-induced diabetic rats involved phosphorylation of GSK3β (Ser-9) in liver
title_sort	hypoglycemic effects of gynura procumbens fractions on streptozotocin-induced diabetic rats involved phosphorylation of gsk3β (ser-9) in liver
publisher	Universiti Kebangsaan Malaysia
publishDate	2012
url	https://eprints.ums.edu.my/id/eprint/20585/1/Hypoglycemic%20effects%20of%20gynura%20procumbens%20fractions%20on%20streptozotocin.pdf https://eprints.ums.edu.my/id/eprint/20585/ https://www.researchgate.net/publication/236030950_Hypoglycemic_effects_of_Gynura_procumbens_fractions_on_streptozotocin-induced_diabetic_rats_involved_phosphorylation_of_GSK3b_Ser-9_in_liver
_version_	1760229734452559872
score	13.211869

Hypoglycemic effects of gynura procumbens fractions on streptozotocin-induced diabetic rats involved phosphorylation of GSK3β (Ser-9) in liver

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