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Screening for antimicrobial activities of bacteria from Antarctica

Naturally produced antimicrobial agent or antibiotics is a low molecular weight natural substance produced by microorganism to inhibit the growth of or kill other microorganisms. Antibiotics from microbes have been used to develop drugs to combat various pathogens. Nevertheless many pathogens hav...

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Main Author: Chung, Hung Hui
Format: Academic Exercise
Language:English
Published: 2008
Online Access:https://eprints.ums.edu.my/id/eprint/20519/1/Screening%20for%20antimicrobial.pdf
https://eprints.ums.edu.my/id/eprint/20519/
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spelling my.ums.eprints.205192018-07-17T05:56:24Z https://eprints.ums.edu.my/id/eprint/20519/ Screening for antimicrobial activities of bacteria from Antarctica Chung, Hung Hui Naturally produced antimicrobial agent or antibiotics is a low molecular weight natural substance produced by microorganism to inhibit the growth of or kill other microorganisms. Antibiotics from microbes have been used to develop drugs to combat various pathogens. Nevertheless many pathogens have developed resistance to the existing antibiotics. Additionally, the pipeline of new drugs is running dry and the incentives to develop new antimicrobials to address the global problems of drug resistance are required. Hence the aims of this study were to isolate and characterize bacteria which produce antimicrobial agent. Bacteria from soil sample in Antarctica were screened for antimicrobial activities by using Escherichia coli 0157: H7, Klebsiella pneumoniae 14x and Salmonella typhimurium (S. Tm 13) as tester strains. Two out of the 356 bacterial isolates exhibited antagonistic effect on the tester strains which were then labeled as SS157 and SR13. Characterization of both strains indicated that their optimal growth temperature was 20°C, salt tolerance up to 3% (w/v) and an optimal growth at pH 7. SR13 was found to be Gram positive rods while Gram stain of SS157 shows that it is a Gram negative coccus. Biochemical tests performed revealed common significant taxa for both as Pseudomonas fluorescens with a percentage identity up to 99.9%. These were then further identified based on their 16S rDNA gene sequences. The 16S rDNA gene was partially sequenced and sequences with length of 1531 and 1536 base pair were obtained for SS157 and SR13 respectively. BLASTn search results revealed that SS157 bears a close phylogenetic relationship to Pseudomonas congelans DSM 14939T( -99%) while SRI 3 was found to bear a close phylogenetic relationship to Pseudomonas tremae CFBP 6111T (-98%). 2008 Academic Exercise NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/20519/1/Screening%20for%20antimicrobial.pdf Chung, Hung Hui (2008) Screening for antimicrobial activities of bacteria from Antarctica. Universiti Malaysia Sabah. (Unpublished)
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
description Naturally produced antimicrobial agent or antibiotics is a low molecular weight natural substance produced by microorganism to inhibit the growth of or kill other microorganisms. Antibiotics from microbes have been used to develop drugs to combat various pathogens. Nevertheless many pathogens have developed resistance to the existing antibiotics. Additionally, the pipeline of new drugs is running dry and the incentives to develop new antimicrobials to address the global problems of drug resistance are required. Hence the aims of this study were to isolate and characterize bacteria which produce antimicrobial agent. Bacteria from soil sample in Antarctica were screened for antimicrobial activities by using Escherichia coli 0157: H7, Klebsiella pneumoniae 14x and Salmonella typhimurium (S. Tm 13) as tester strains. Two out of the 356 bacterial isolates exhibited antagonistic effect on the tester strains which were then labeled as SS157 and SR13. Characterization of both strains indicated that their optimal growth temperature was 20°C, salt tolerance up to 3% (w/v) and an optimal growth at pH 7. SR13 was found to be Gram positive rods while Gram stain of SS157 shows that it is a Gram negative coccus. Biochemical tests performed revealed common significant taxa for both as Pseudomonas fluorescens with a percentage identity up to 99.9%. These were then further identified based on their 16S rDNA gene sequences. The 16S rDNA gene was partially sequenced and sequences with length of 1531 and 1536 base pair were obtained for SS157 and SR13 respectively. BLASTn search results revealed that SS157 bears a close phylogenetic relationship to Pseudomonas congelans DSM 14939T( -99%) while SRI 3 was found to bear a close phylogenetic relationship to Pseudomonas tremae CFBP 6111T (-98%).
format Academic Exercise
author Chung, Hung Hui
spellingShingle Chung, Hung Hui
Screening for antimicrobial activities of bacteria from Antarctica
author_facet Chung, Hung Hui
author_sort Chung, Hung Hui
title Screening for antimicrobial activities of bacteria from Antarctica
title_short Screening for antimicrobial activities of bacteria from Antarctica
title_full Screening for antimicrobial activities of bacteria from Antarctica
title_fullStr Screening for antimicrobial activities of bacteria from Antarctica
title_full_unstemmed Screening for antimicrobial activities of bacteria from Antarctica
title_sort screening for antimicrobial activities of bacteria from antarctica
publishDate 2008
url https://eprints.ums.edu.my/id/eprint/20519/1/Screening%20for%20antimicrobial.pdf
https://eprints.ums.edu.my/id/eprint/20519/
_version_ 1760229725902471168
score 13.211869

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