Cover Image

Molecular diagnostics of plasmodium parasites

Malaria is a major public health problem in tropical and subtropical areas, especially in Southeast Asia region, caused by any of five species of Plasmodium (P. falciparum, P. vivax, P. malariae, P. ovale and P. knowlesi). It still remains a leading cause of morbidity and mortality worldwide. The...

Full description

Saved in:
Bibliographic Details
Main Author: Cheronie Shely Stanis
Format: Thesis
Language:English
Published: 2015
Subjects:
QR Microbiology
Online Access:https://eprints.ums.edu.my/id/eprint/19566/1/Molecular%20diagnostics%20of%20plasmodium%20parasites.pdf
https://eprints.ums.edu.my/id/eprint/19566/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.ums.eprints.19566
record_format eprints
spelling my.ums.eprints.195662018-03-27T06:32:25Z https://eprints.ums.edu.my/id/eprint/19566/ Molecular diagnostics of plasmodium parasites Cheronie Shely Stanis QR Microbiology Malaria is a major public health problem in tropical and subtropical areas, especially in Southeast Asia region, caused by any of five species of Plasmodium (P. falciparum, P. vivax, P. malariae, P. ovale and P. knowlesi). It still remains a leading cause of morbidity and mortality worldwide. The aim of this study was to design new molecular markers for identification of Plasmodium spp. and compare the parasite species identification using the molecular methods (nested PCR and new multiplex PCR) and microscopy. Blood samples on filter papers were subject to conventional PCR methods using primers designed by us in multiplex PCR and compared with primers of nested PCR of Singh et at. (1999; 2004), as well as with microscopic identification. Of the 109 samples identified as malaria positive by microscopic examination, 15 samples were positive for P. falciparum, 14 for P. vivax, six for P. knowlesi, 72 for P. malariae, two for mixed infection of P. falciparum/ P. malariae and 20 which serve as negative controls. Both multiplex PCR and nested PCR detected 12 of P. falciparum single infections. For P. vivax, nine were detected by multiplex and 12 detected by nested PCR. For 72 P. malariae cases, nested PCR detected two as P. falciparum, 57 as P. knowlesi seven as P. malariae and six negative. However, multiplex PCR identified two as P. falciparum, 57 as P. knowlesi, ten as P. malariae and three negative. For 28 samples which had been identified as P. falciparum, P. malariae and P. vivax by microscopy, they were identified as negative by both multiplex PCR and nested PCR. This shows multiplex PCR is highly sensitive and specific. The multiplex PCR assay is faster than nested PCR and could be used as an alternative molecular diagnosis for the Identification and detection of all Plasmodium spp. as it has shorter the time required for screening a large number of samples. 2015 Thesis NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/19566/1/Molecular%20diagnostics%20of%20plasmodium%20parasites.pdf Cheronie Shely Stanis (2015) Molecular diagnostics of plasmodium parasites. Masters thesis, Universiti Malaysia Sabah.
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
topic QR Microbiology
spellingShingle QR Microbiology
Cheronie Shely Stanis
Molecular diagnostics of plasmodium parasites
description Malaria is a major public health problem in tropical and subtropical areas, especially in Southeast Asia region, caused by any of five species of Plasmodium (P. falciparum, P. vivax, P. malariae, P. ovale and P. knowlesi). It still remains a leading cause of morbidity and mortality worldwide. The aim of this study was to design new molecular markers for identification of Plasmodium spp. and compare the parasite species identification using the molecular methods (nested PCR and new multiplex PCR) and microscopy. Blood samples on filter papers were subject to conventional PCR methods using primers designed by us in multiplex PCR and compared with primers of nested PCR of Singh et at. (1999; 2004), as well as with microscopic identification. Of the 109 samples identified as malaria positive by microscopic examination, 15 samples were positive for P. falciparum, 14 for P. vivax, six for P. knowlesi, 72 for P. malariae, two for mixed infection of P. falciparum/ P. malariae and 20 which serve as negative controls. Both multiplex PCR and nested PCR detected 12 of P. falciparum single infections. For P. vivax, nine were detected by multiplex and 12 detected by nested PCR. For 72 P. malariae cases, nested PCR detected two as P. falciparum, 57 as P. knowlesi seven as P. malariae and six negative. However, multiplex PCR identified two as P. falciparum, 57 as P. knowlesi, ten as P. malariae and three negative. For 28 samples which had been identified as P. falciparum, P. malariae and P. vivax by microscopy, they were identified as negative by both multiplex PCR and nested PCR. This shows multiplex PCR is highly sensitive and specific. The multiplex PCR assay is faster than nested PCR and could be used as an alternative molecular diagnosis for the Identification and detection of all Plasmodium spp. as it has shorter the time required for screening a large number of samples.
format Thesis
author Cheronie Shely Stanis
author_facet Cheronie Shely Stanis
author_sort Cheronie Shely Stanis
title Molecular diagnostics of plasmodium parasites
title_short Molecular diagnostics of plasmodium parasites
title_full Molecular diagnostics of plasmodium parasites
title_fullStr Molecular diagnostics of plasmodium parasites
title_full_unstemmed Molecular diagnostics of plasmodium parasites
title_sort molecular diagnostics of plasmodium parasites
publishDate 2015
url https://eprints.ums.edu.my/id/eprint/19566/1/Molecular%20diagnostics%20of%20plasmodium%20parasites.pdf
https://eprints.ums.edu.my/id/eprint/19566/
_version_ 1760229598185914368
score 13.211869

Similar Items